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Showing content with the highest reputation since 03/03/23 in all areas

  1. 6 points
    Got a range of nice to ok Pedro tips after a major pruning session. They'd make excellent grafting stock. $10 a tip or the lot for $50. Postage extra.
  2. 5 points
    Hi All, been a while since my last lurk around the forums I've got some Courtii seed available for purchase. $25 per 30+ seed pack. Or $1 each. Harvested January 2022 from seed grown trees in southern Tasmania. Message for inquiry ✌
  3. 5 points
    I feel the succulent dimension holds an interesting element in potentially having more therapeutically useful "in the real world" dimensions than more commonly touted plant medicines for their often more gentle but occasionally empathogenic edge. Also, the combination of often pro-cognitive (PDE4 inhibition seen with mesembrine-type alkaloids) with potent antidepressant effects meets an unmet demand. Part 1: Background and 'citizen analysis' using readily available materials Some Aizoaceae have been analysed [1]: I wanted to see if it was feasible to get some rough idea of the phytochemistry of a plant using only readily available materials eg. making it suitable for citizen science. I've been curious about simple TLC and paper chromatography as tools, particularly how feasible 'citizen TLC analysis' of plants is using a suitable paper, readily available solvent systems and simple visualisation techniques, compared to more conventional TLC. Can simple maceration of a medicinal plant in a readily available benignish solvent, concentration, spotting on common craft materials, developing the plate and OTC visualisation give some rough idea of the phytochemistry of a medicinal plant? Sceletium has in the past been analysed via conventional TLC [2, 3] and more advanced techniques [4] It took about 30 trials to find a readily available solvent system and semi-suitable paper - after many failed attempts, I found an 'etch art paper' that seemed the best I could find. I'm still to follow up with confirmation on silica TLC plates but it seems to give *a very rough idea* Sceletium tortuosum (unfermented) was used as a reference. This gives a main compound Rf = 0.5-0.54 on the craft paper. I particularly wanted to research D. bosseranum and Trichodiadema stellatum Method: Sample preparation: Samples were dehydrated at 70 deg. C and powdered. The samples were macerated in isopropanol. If no phenolic constituents were expected, NaOH q.s. was used to basify, otherwise basified with ammonia solution. The isopropanol solutions were concentrated to a small sample. Samples were spotted onto a plate, either a suitable craft paper, or silica gel plates. Acetone was used to elute the plate and I2 vapour (from OTC tincture) used as a visualisation technique. Crude technique - Craft Paper Note: this technique is limited in effectiveness and I suggest if you're that keen to play around with something like this, I'd suggest you just stick with silica gel plates to save A LOT of hassle. Delosperma bosseranum Sceletium tortuosum (unfermented) was compared to D. bosseranum. A compound with the same Rf as Sceletium was noted along with other constituents. Trichodiadema stellatum (syn. barbatum) Sceletium tortuosum (unfermented) was compared to D. bosseranum. A compound with the same Rf as Sceletium was noted along with other constituents. Mesembryanthemum cordifolium (syn. Aptenia cordifolia) This has been studied and seems to contain 4,5-dihydro-4’-O-methylsceletenone and 4’-O-methylsceletenone along with flavanoids, tannins, phenols, saponins, and cinnamic acid derivatives (and esters) The characteristic Sceletium constituent was absent in these but other different compounds were present. Will follow up with more conventional silica TLC which will hopefully have more easily reproducible results
  4. 5 points
    This is what you get with people setting themselves up as healers when they do not have the training and experience to do so - a two week residential program will never teach you anything and you are delusional to think that you have the skills to cope with crisis and the real skill is managing the crises that is certain arrive. In a culture were expertise and experience is given short thrift in the quest for money, status and influence. Such things are bound to happen. Such people are playing into the hands of those in power giving them the justification to criminalize all things until a commercially patented product can be sold to the plebeians.
  5. 5 points
    After some peer scrutiny on the initial attempt and revisions, here is a rough draft. mesemb paper V2.pdf
  6. 4 points
    Now that spring is almost upon us I thought I’d share the method I use to germinate Acacia Phlebophylla seeds. It has taken a few years of trial and error but now I have it down pat. I’ve got it to 90%+ germination rate all in the first month of spring. It certainly isn’t the only method but it works for me and I thought sharing might encourage others to give it a try. I like to start the process early August (as it takes about 4 weeks for them to start germinating) to take full advantage of the spring/summer growing season. I use a razor blade and take a tiny nick off the hilum end of the seed, this is where the root will come through. Be careful not to take too much off you only need to get through outer coat. Once they are all scarified I then soak them for 24hrs in previously boiled but COLD water. Boiling water at this point will kill your seeds if using this method. After 24hrs and seeds have swelled up, I now put them in a plastic Chinese container between two sheets of damp paper towel (I like viva brand as it holds a lot more moisture than cheaper towel), all spaced out so they aren’t touching. I put the lid on and place them in the fridge.  They will germinate in the fridge and throw out a tap root but it normally takes about four to six weeks for the first ones to start. Once they grow their little root (2 or 3mm, don’t let it get too long) I pick them out of the container and put the rest back in the fridge, checking them every few days. It normally takes a few weeks for them all to germinate once they’ve started. I then very carefully with a pair of tweezers peel off the outer coating of the seed and plant them in a seedling tray just under the soil with the root facing down. I put the seedling tray in the hothouse watering daily as seedling trays dry out quickly. Your little seedlings will be up in a few days.  soil mix used is pretty simple, 3 parts Bunnings native potting mix to 1 part river sand, with a handful of soil from the base of one of your local acacias as a rhizobia inoculant mixed in to every seedling tray. They take a couple of months to get their first adult leaves but once they do they grow fairly quickly. I also then start fertilising them with swift grow (swiftgrow.com.au) every couple of weeks. Happy gardening 
  7. 4 points
    The problem with a lot of these kinds of people who set themselves up as facilitators is they don't believe in traditional medicine so they thought they knew better than to call an ambulance. Nothing a little guitar playing and chanting can't fix... Criminally negligent not to be prepared for a medical emergency and act fast.
  8. 4 points
    Happy 420 to yerz all. Here's a nice pic from the internet to cheer you up if you're feelin' blue like me.
  9. 4 points
    Citizen Plant Science - Exploring the Aizoaceae Despite a long history of traditional use as medicines, the Aizoaceae remain an under-studied family of plants [1]. They often contain phytocompounds such as alkaloids, flavonoids, steroids, and their related intermediary compounds, one of the commonly found alkaloids is the mesembrine-type alkaloids [2,3]. These plants are well known in traditional medicine systems for antidepressant, anxiolytic, antimicrobial, anti-inflammatory, and antioxidant effects. Sceletium tortuosum (Figure 1.) contains mesembrine and derivatives (Figure 2.), an alkaloid not typically found in the emarcidum-type Sceletium spp. Other plants such as Delosperma pruinosum, and Delosperma pottsii [2] appeared to have some level of mesembrine but while Mesembryanthemum cordifolium syn. Aptenia cordifolia was initially thought to contain some levels of mesembrine, it seems instead to contain derivatives such as mesembrane sceletenone derivatives [4] which have in initial studies shown antidepressant activity superior to imipramine [5]. Figure 1. S. tortuosum Figure 2. Mesembrine and derivatives. Other Sceletium spp. have been traditionally used, S. emarcidum "was valued as highly as S. tortuosum in Southern Africa by different tribes and makes a very good Kougoed product". While apparently devoid of mesembrine, it seems to contain 4'-O-demethylated mesembrine-type alkaloids [6]. Mesembrine has been found in Amaryllidaceae such as Narcissus pallidulus and Narcissus triandrus. Other Mesembryanthemums such as M. crystallinum and M. nodiflorum have been suggested to contain some levels of mesembrine. More recently, Lampranthus spp. (Figure 3.) have been specifically marketed as Sceletium substitutes 'Chinese Kanna', or used as adulterants. These seem to contain mesembrenol and low levels of mesembrenone. Figure 3. Lampranthus and mesembrenol One early explorer called S. tortuosum ‘the greatest Clearer of the Spirits, and the noblest Restorative in the World’. Traditional use of Sceletium in South Africa often involves the collection and fermentation of the plant material, which has been studied and lead to greater levels of mesembrine and changes to levels of mesembrenone [7] and reduce oxalate levels. Mesembrine is a potent serotonin reuptake inhibitor (Ki = 1.4 nM) and to a lesser extent PDE4 inhibitor. Other notable mesembrine-type alkaloids include mesembrenone (also a potent serotonin reuptake inhibitor [Ki = 27 nM] and a more potent PDE4 inhibitor than mesembrine itself), and mesembranol. Another assay found that Sceletium tortuosum comprising of > 70% (w/w) stabilized mesembrine was a monoamine releasing agent [8]. The pharmacological properties of mesembrine and it's derivatives are quite diverse, showing anti-inflammatory, cytoprotective, VMAT-2 upregulation, mild inhibition of AChE, mild MAO inhibition, limited reuptake of NE and DA at high concentrations. Mesembrenol and mesembrenone seem to also share the SERT inhibitory and PDE4 inhibitory activitty. Clinically, the Sceletium spp. have shown antidepressant, anxiolytic and cognitive enhancement effects, case studies demonstrating increased attention, focus, and motivation while a patient with “a baseline mood of depression alternating with anxiety” felt “more focused, more engaged, and not so socially distant” after ten days [9]. Species such as Delosperma bosseranum (Figure 4) have been suggested by members of the ethnobotanical community to offer similar effects to Sceletium spp. but remain uncharacterised phytochemically and pharmacologically. Others such as Trichodiadema spp. (Figure 4), traditionally used as beer making roots known as 'Karee Moer' (T. barbartum syn. stellatum) as suggested to contain mesembrine but a detailed analysis is lacking (limited to positive alkaloid tests). Figure 4. The prototypical mesembrine containing plant, Sceletium and D. bosseranum and T. barbatum The purpose of this citizen science was to explore a diverse range of Aizoaceae for mesembrine-type alkaloids, including uncharacterised ones such as Delosperma bosseranum and Trichodiadema barbatum and screen diverse Mesembryanthemums for possible mesembrine-type alkaloids. [1] https://doi.org/10.1016/j.jep.2022.115988 [2] https://doi.org/10.1016/j.phytochem.2019.112061 [3] https://doi.org/10.1076%2Fphbi. [4] https://def-sa.com/wp-content/uploads/2020/08/Gaffney_Candice_D_2006_0.pdf [5] https://doi.org/10.1080/14786419.2020.1788019 [6] https://doi.org/10.3389%2Ffnut.2022.819753 [7] https://doi.org/10.1016/j.sajb.2018.10.011 [8] https://patents.google.com/patent/US20160038551A1/en [9] https://www.researchgate.net/publication/328942189_Kabbo's_Kwain_The_Past_Present_and_Possible_Future_of_Kanna A range of Aizoaceae (Silica, I2 visualisation) gave diverse phytochemical profiles Trichodiadema stellatum (root) TS Trichodiadema bulbosum (leaf/stem) TB Sceletium tortuosum (root) ST Sceletium emarcidum (leaf/stem) SE Aptenia cordifolia AC ? Unknown Mesemb. Drosanthemum floribundum Dros. Mesembryanthemum (Red) [?Lampranthus] MR Mesembryanthemum (Gold) MG Mesembryanthemum (Yellow, squarish stems) MY Mesembryanthemum (White) MW D. bosseranum DB D. floribundum DF 1. acetone elution 2. acetone:white spirits:1:1 elution To TLC soon Tetragonia tetragonioides Mesembryanthemum crystallinum Lampranthus spectabilis (Red) D. echinatum
  10. 3 points
    A few mid-cut sections of this special scopulicola available, for anyone interested in propagating. I've never seen a scop with such pronounced horizontal strata, which definitely caught my eye (and I've already got quite a few scop varieties, including some real stunners). It's absolutely spineless to the touch, with deeply recessed aureoles. The mother is no longer, unfortunately. These chunks are fat and heavy (CD in last pic. for reference). PM if interested.
  11. 3 points
    https://www.sciencedirect.com/science/article/abs/pii/S0378874120331986 in rodents, at least.
  12. 3 points
    Dunno if you've noticed, but it's not just your justice that's being sidelined. Going to a GP these days is a lottery, very few winners, lots of lazy diagnosis and being sent home and told you're imagining it. Or worse, drug seeking- even when you haven't asked for drugs. It'll get your appointment terminated straight up and go on your record for future appointments IMO it's a major contributor to Australia having a high rate of chronic illness. Nobody bothers to investigate until you're almost dead I do believe vax injury is a thing, but so is longCOV. There are no perfect answers yet. Hope your symptoms vanish fast and you can heal
  13. 3 points
    Unfortunately there are many stories like yours. I hope you get some relief from the tinnitus with nice music, sounds of nature, or some form of distractions. All the best PH.
  14. 3 points
    if you experience, some unwanted side effects from the vac, you can't complain to anybody, or any institution. i tried to contact the tga, all you are allowed to talk to is a phamacist, who say's i'm, sorry for what happend to you. in the first test, nobody complained about tinitus, but now one if 5000 get's it. there is a web page for serious side effects, and a goverment pay out scheme, but for non life threatening side effects, you get no help, they just all ignore your pleas. the astra seneca vac, gave me tinitus, and i still have it to this day. when i had covid, the tinitus intensified, but now it's not very loud again. it's a very anoying condition, i feel i get no justice....
  15. 3 points
    I actually feel that the TGA's agenda towards scheduling (a step or two down the ladder just like psilocybin and MDMA) will keep going on its merry way, regardless of what our communities say, as we aren't corporate/venture capitalists/pharma giants with the cash to lean hard where they need to. Smoke and mirrors to distract and obscure themes like ecology, preservation, self sufficiency, self-care and sovereignty over consciousness, as we move inexorably closer to pay to play, synthetics and production line manufacture of anything that can be sold for a few bucks from the psychedelic realm...
  16. 3 points
    I've been busy expanding on this research, hopefully it's of interest/inspiration to others: So far the research extends to: Citizen TLC Phytochemical Screening of Diverse Mesembryanthemums https://pdfhost.io/v/gugGrtnCj_ Phytochemistry of Aptenia https://pdfhost.io/v/dq6SpR9nM_aptenia Citizen TLC analysis of marketed commercial Sceletium tortuosum products: https://pdfhost.io/v/SVvI3PQwb_TLC_commercial_sceletium_produc Using TLC to guide discovery of hypothetical new Kanna substitutes https://pdfhost.io/v/A4u.08qVQ_kanna_substitutes_TLC Utilising yeasts to encourage the bioconversion of mesembrine-type alkaloids yeasts to encourage bioconversi | PDF Host Initial TLC study of an uncharacterised Sceletium ('Little Karoo') https://pdfhost.io/v/.ASjnTMdo_Initial_TLC_study_of_an_unchara Sceletium Chemotypes - Characterisation of a 'Superior Quality' Sceletium product sceletium chemotypes | PDF Host Reagent analysis of phytochemicals - application to Sceletium spp and Mesembryanthemums. Reagent analysis of phytochemicals - application to Sceletium spp and Mesembryanthemums. | PDF Host
  17. 3 points
    It's getting cold in Melbourne now, Sub are poping; maybe we need an indoor meet around a fire this winter; anyone got a cool property
  18. 3 points
    Yes, this is a hard one, Psychedelics are no longer fringe topics, and our community is being showcased for good or for bad. We need to be ready for more of this as the TGA decision has the media working on Psychedelics stories left, right and centre. Liam has done a lot of good work for the community, and a couple of us gave an interview for the book (that this chapter was lifted from); sadly, the use of our words fell beyond our control as the media often does. We thought is was a book for plant nerds not the major media spaces, but that life, sadly. Attention is a good and bad thing; even on a bad day I think Liam has a better voice generally for the community than many out there. Liam has volunteered much of the last 10 years of his life trying to help this space, however, he is very much still learning like we all are. This situation could have been better on many front and i not just talking about Liam here, even myself, i made mistakes around this, and need to live with that. It's a balance between trying to show we are good people and we have good gardens and good wellness stories and the media diving in like vultures on our community and gardens. The chapter in question here was Liam chapter was his own story, associated with his work with us, but not a direct part of his role with EGA. At EGA we generally do not do media. We rather focus on making our own content, and self-publishing. This has again made us think hard about the next time we do or will. I support Liam and his work more generally, I feel he is an asset to the community and I know he released a statement on that matter, so I recommend reading that as part of this discussion.
  19. 3 points
    I was wondering if anyone can help ID these. I had help with an ID here a couple of years ago and transffered some spores of what I think was a positive p.sub ID, to my new place. I have cultivated a nice little patch in garden mulch which I had fed with cardboard and eucy mulch over the past few years. the mycelum patch now is a solid 2m long by about 1m wide. With a nice little misting system that gives them a regular spray. I am in the ACT and they just started flushing in the last week. I have watch the entheogenesis ID vids, but I just want to make sure. Any answers are greatly appreciated.
  20. 3 points
    While the Sceletium has been showing continued promise for modulating mood and other dimensions, I've been experiencing significant and quite debilitating catatonic features for a long time and poor sleep quality but was personally hesitant to initiate the conventional lorazepam benzodiazepine therapy for catatonia due to the addictive nature of BZDs and past issues with addiction. Decided to try higher dose baicalin extract and akin to the rapid resolution of catatonia seen with a lorazepam-challenge, there was robust rapid acute resolution of the catatonic features, agitation and somewhat improved sleep quality. Just able to feel slightly chill for once. Very early days but it seems to be quite useful and also stabilising for one's mental state. The improvement of catatonic features was noted on second dose etc but as the features have been ingrained quite heavily, there's occasional return of posturing etc Subjective downsides: - waking up in the morning is harder, significant cognitive clouding etc (anything even slightly sedative does this to me) - reduced CNS arousal negatively impacts mental state The baicalin also shows diverse additional features over lorazepam that intrigue me and are relevant to my situation. Found about 1/2 tsp of 85% baicalin was sufficient S. baicalensis and it's primary active constituent baicalin has diverse pharmacological properties. In particular, baicalin seems to have promising CNS activity [1]. Antidepressant- and anxiolytic-like properties (the latter mediated through the activation of benzodiazepine binding site of GABAA receptors) Inhibits prolyl oligopeptidase dose-dependently, having potential benefits for schizophrenia, bipolar affective disorder, and related neuropsychiatric diseases [2]. Can potentially be used to treat dopaminergic dysfunction-associated CNS diseases (incl. neurodegenerative and ADHD). It's able to protect dopaminergic neurons and modulate brain dopamine levels, thus serving as a potentially effective novel treatment for ADHD [3]. In schizophrenia, addition of baicalin (1.5g/day) to atypical antipsychotics led to greater improvements in both primary and secondary negative symptoms than those treated with antipsychotics alone - seems to have efficacy for predominant negative symptoms and in improving cognitive function [4]. Able to facilitate remyelination in various models of CNS disorders and suppress neuroinflammation [5] Baicalin can: - pass through the blood–brain barrier - stimulate neurogenesis - promote neural differentiation and inhibit neuronal apoptosis - inhibit neuroinflammation and oxidative stress - promote CNS myelin repair Shown to be relatively nontoxic when given orally
  21. 3 points
  22. 3 points
    This doesn't make sense to me: "The reason why people are out “spreading this mycelium around is because we all feel the corporate thing coming and we don't like it,” he adds. Steve claims to have single-handedly created hundreds of magic mushroom patches in the US: “This is a way that I can make the experience more available. But there are a lot of people doing bigger and more elaborate things.” not defending 'the corporate thing' by any means, but I don't think commercial interests in magic mush will make it any harder to go out picking than it already isn't
  23. 3 points
  24. 3 points
  25. 2 points
    From about the 50-minute mark, investigative report into TGA approval process, discussion of who benefits, etc. https://www.abc.net.au/radionational/programs/sundayextra/sunday-extra/102057916