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Fractalhead

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    Botany, Mycology, Neuroscience and applications to consciousness, Pharmacology, Chemistry, Sustainable technology, Music, Dancing, Surfing, Diving, and lots of other stuff too numerous to mention...

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  1. Fractalhead

    Seasonal variation in alkaloid levels of Acacia

    I've heard that the highest alkaloid levels in acacia generally occur during the peak of the growing season when there is plenty of water (ie. levels are low during long periods of drought such as during summer in the temporate areas and winter in the tropics and subtropics.
  2. Fractalhead

    smokable lsa's from common house plant!!!

    Certain members of the convolulaceae have been found to produce nortropane alkaloids (eg. calystegines) in the roots only. Could the smoking of roots be a clue? Personally, I'd be very suprised if the actives in convolvulaceae smoke were ergolines. Surely they would be disintegrated by the heat.
  3. Fractalhead

    HBWR question

    So how low is this baseline T? It sounds like its high time for an LC/MS time course experiment If the drop-off does equilibrate to a stable baseline this could suggest that the decrease in potency could be the result of a shift in metabolic equilibrium between the seed on the vine and off the vine. Perhaps there is actual enzymatic metabolism going on in the seed once its picked and the fold decrease in potency depends on the starting levels of certain enzymatic substrates at the point of harvest. Alternatively, there may be more than one major active, one more stable than the other. The final baseline activity (and degradation curve) may depend on the initial ratios of the actives which in turn may depend on genetics and/or epigenetic factors. Hmmm interesting stuff... looks like I might have to re-investigate
  4. Fractalhead

    HBWR question

    quote: Or is the name just an indication of its alkaloid content? yes. Hehe. If there are still Fractal seeds out there, they are best planted in the ground so that they can grow into plants with seeds showing alkaloid profiles that warrant the name. The freshest original 'Fractal' seed is about two years old now and therefore more or less devoid of goodness (unless someone out there worked out some kind of preservation process I wonder if anyone has any seeding plants from those yet???
  5. Fractalhead

    Nutmeg rocks my world

    Yeah tort re apiol - that's what i was thinking. Theres also the tetramethoxy allylbenzene in there two. While we're on the topic of oils... does anyone have any putative myristicin containing Zieria sub species samples they want checked do they? I'll swap you a free analysis for the GPS coords As you pointed out Tort it would be good to get some w/v breakdowns. As much as it would be nice to have standards to make standard curves on the GC/MS, I think a more readily attainable way would be to set up my GC/FID More soon...
  6. Fractalhead

    Nutmeg rocks my world

    Hehe. Wow!! so many willing participants... Yes I think that absta(i?)nence would be a good scientifically responsible thing to maintain in the week or so prior to the experiment. Maybe the more energetically inclined lab rats could even do some vigorous muscular exercise and we can compare their stats with the couch cruisers' Who knows, we might even come up with some novel mass spectra... So Tort, do you reckon refined parsley would be a satisfactory substitute? I remember you saying something about vertigo after some refined dill oil. Something about the tetra-subs? Also T, what kind of dose do you think would be a good starting point in terms of dose if we're assuming a 90% w/v myristicin oil? I've got that paper from Sasha where they achieve a mild effect from 400mg myristicin but I'm not sure whether they knew about your exercise theory in those days Maybe we can do two experiments with different doses. This is going to be fun! Fractal
  7. Fractalhead

    Nutmeg rocks my world

    Torsten, just glancing over your analysis it looks like you came to the same conclusions I did... except for the bit about their being no elemicin. The mace oil definitely had 'some' elemicin. Unfortunately, at the threshold settings I ran that nist search on, there wasn't quite enough signal from elemicin to be included in the search. I actually injected a very dilute solution of the oil in methanol. If I'd changed the settings or upped the sample concentration elemicin would have turned up for sure. I found a peak after myristicin that was very small and did a manual search that hit elemicin with a high match score. This same peak has turned up in just about every myristica oil analysed so far. There are def differences in the ratios of the three main allylbenzenes (s, m and e) in different oils but every oil i've tested now (about 5 or six), the myristicin peak is about 5-10 fold larger than the S peak. When I have time I'll collate all the data and post it up. So if there were some Myrista sp. essential oil 'high boiling fraction' available, who might be interested? Who knows, maybe you can get some cash back when you send your 'mid-party' urine sample back to me so I can analyse it for metabolites that might provide some clues as to the metabolic pathways going on. No seriously, if i get some refined oils together, would anyone be interested in being involved in a big community science experiment? I'm thinking if people each pay a certain amount of money to take part and then I send out a sample of refined nutmeg oil (with all the nasty lighter boiling crap like pinene etc removed). To encourage everyone to send back a urine sample, I could send back some extra treats when I receive the urine sample (no rude practical jokes please . Some of the money will go towards buying the disposable solid phase liquid extractors that will concentrate all the basic metabolites prior to analysis. If we all work together we could really nail this metabolic nutmeg madness once and for all
  8. Fractalhead

    new MS browser

    ohhh man! ad i thought you had some new mass spectrometry database for us ;(
  9. Fractalhead

    Nutmeg rocks my world

    Hi Tort, I've analysed those Myristica sp. ois of yours on a gc/ms. Am I right in guessing that one of them was mace and one of them nutmeg oil. You were very cryptic with your M and N labelling I guess it was a nice example of a blind study... I'll post the library search qualitative reports below: HERE is the 'N' oil data: Pk# RT Area% Library/ID Ref# CAS# Qual _____________________________________________________________________________ 1 5.98 15.63 D:DatabaseNIST02.L 1R-.alpha.-Pinene 15161 007785-70-8 90 1S-.alpha.-Pinene 15160 007785-26-4 87 Cyclohexene, 4-methylene-1-(1-meth 15299 000099-84-3 81 ylethyl)- 2 6.04 26.91 D:DatabaseNIST02.L 1R-.alpha.-Pinene 15163 007785-70-8 95 1R-.alpha.-Pinene 15161 007785-70-8 95 Bicyclo[3.1.1]hept-2-ene, 2,6,6-tr 15351 002437-95-8 93 imethyl-, (.+/-.)- 3 6.36 6.02 D:DatabaseNIST02.L Bicyclo[3.1.0]hex-2-ene, 4-methyl- 15349 028634-89-1 91 1-(1-methylethyl)- Bicyclo[3.1.0]hexane, 4-methylene- 15348 003387-41-5 91 1-(1-methylethyl)- Bicyclo[3.1.0]hexane, 4-methylene- 15354 003387-41-5 91 1-(1-methylethyl)- 4 6.46 19.40 D:DatabaseNIST02.L .beta.-Phellandrene 15173 000555-10-2 90 .beta.-Pinene 15150 000127-91-3 81 Cyclohexane, 1-methylene-4-(1-meth 15307 000499-97-8 80 ylethenyl)- 5 6.97 11.07 D:DatabaseNIST02.L Limonene 15127 000138-86-3 52 Cyclohexanol, 2-methyl-5-(1-methyl 25625 000619-01-2 38 ethenyl)- 1,3,7-Octatriene, 3,7-dimethyl- 15218 000502-99-8 38 6 7.18 2.86 D:DatabaseNIST02.L 3-Carene 15131 013466-78-9 42 1,4-Cyclohexadiene, 1-methyl-4-(1- 15330 000099-85-4 42 methylethyl)- Cyclohexene, 1-methyl-5-(1-methyle 15336 001461-27-4 42 thenyl)-, ®- 7 7.77 1.29 D:DatabaseNIST02.L Pentafluoropropionic acid, pentade 149597 1000280-07-5 58 cyl ester Pentafluoropropionic acid, octadec 159526 1000280-07-7 49 yl ester 1-Dodecanol, 3,7,11-trimethyl- 75210 006750-34-1 49 8 8.30 4.28 D:DatabaseNIST02.L 3-Cyclohexen-1-ol, 4-methyl-1-(1-m 25652 000562-74-3 90 ethylethyl)- 3-Cyclohexen-1-ol, 4-methyl-1-(1-m 25686 020126-76-5 87 ethylethyl)-, ®- 3-Cyclohexen-1-ol, 4-methyl-1-(1-m 25683 020126-76-5 76 ethylethyl)-, ®- 9 9.16 1.69 D:DatabaseNIST02.L 1,3-Benzodioxole, 5-(2-propenyl)- 30384 000094-59-7 92 1,3-Benzodioxole, 5-(1-propenyl)- 30379 000120-58-1 91 1,3-Benzodioxole, 5-(1-propenyl)- 30382 000120-58-1 91 10 10.68 10.85 D:DatabaseNIST02.L 1,3-Benzodioxole, 4-methoxy-6-(2-p 50186 000607-91-0 92 ropenyl)- 1,3-Benzodioxole, 4-methoxy-6-(2-p 50187 000607-91-0 91 ropenyl)- 1,3-Benzodioxole, 4-methoxy-6-(2-p 50188 000607-91-0 89 ropenyl)- and here id the 'M' oil: Pk# RT Area% Library/ID Ref# CAS# Qual _____________________________________________________________________________ 1 5.98 20.45 D:DatabaseNIST02.L 1R-.alpha.-Pinene 15161 007785-70-8 95 1S-.alpha.-Pinene 15160 007785-26-4 94 Bicyclo[3.1.1]hept-2-ene, 3,6,6-tr 15289 004889-83-2 91 imethyl- 2 6.37 4.57 D:DatabaseNIST02.L Bicyclo[3.1.0]hex-2-ene, 4-methyl- 15349 028634-89-1 87 1-(1-methylethyl)- Bicyclo[3.1.0]hexane, 4-methylene- 15354 003387-41-5 87 1-(1-methylethyl)- 1S-.alpha.-Pinene 15160 007785-26-4 83 3 6.46 21.75 D:DatabaseNIST02.L .beta.-Pinene 15146 000127-91-3 91 Cyclohexene, 4-methylene-1-(1-meth 15299 000099-84-3 91 ylethyl)- 1R-.alpha.-Pinene 15161 007785-70-8 86 4 6.79 3.10 D:DatabaseNIST02.L 1,4-Cyclohexadiene, 1-methyl-4-(1- 15330 000099-85-4 87 methylethyl)- Cyclohexene, 4-methyl-3-(1-methyle 15308 099805-90-0 86 thylidene)- Bicyclo[4.1.0]hept-3-ene, 3,7,7-tr 15344 000498-15-7 60 imethyl-, (1S)- 5 6.87 2.21 D:DatabaseNIST02.L Benzene, 4-ethyl-1,2-dimethyl- 14364 000934-80-5 42 Benzene, 1-methyl-2-(1-methylethyl 14406 000527-84-4 38 )- Benzene, 1-methyl-4-(1-methylethyl 14401 000099-87-6 38 )- 6 6.91 3.36 D:DatabaseNIST02.L Cyclohexene, 1-methyl-4-(1-methyle 15340 005989-54-8 49 thenyl)-, (S)- Limonene 15128 000138-86-3 46 D-Limonene 15136 005989-27-5 41 7 6.95 8.88 D:DatabaseNIST02.L Cyclohexene, 1-methyl-5-(1-methyle 15288 013898-73-2 55 thenyl)- 1,3,7-Octatriene, 3,7-dimethyl- 15218 000502-99-8 45 1S-.alpha.-Pinene 15160 007785-26-4 38 8 7.18 4.23 D:DatabaseNIST02.L Bicyclo[4.1.0]hept-3-ene, 3,7,7-tr 15344 000498-15-7 55 imethyl-, (1S)- 1,4-Cyclohexadiene, 1-methyl-4-(1- 15329 000099-85-4 55 methylethyl)- 1,4-Cyclohexadiene, 1-methyl-4-(1- 15330 000099-85-4 55 methylethyl)- 9 7.44 3.49 D:DatabaseNIST02.L Silane, trichlorodocosyl- 163388 007325-84-0 30 3-Hexadecyloxycarbonyl-5-(2-hydrox 158227 1000222-82-8 22 yethyl)-4-methylimidazolium ion Pentadecane, 1-bromo- 111968 000629-72-1 18 10 8.30 6.48 D:DatabaseNIST02.L 3-Cyclohexen-1-ol, 4-methyl-1-(1-m 25686 020126-76-5 87 ethylethyl)-, ®- 3-Cyclohexen-1-ol, 4-methyl-1-(1-m 25683 020126-76-5 87 ethylethyl)-, ®- 3-Cyclohexen-1-ol, 4-methyl-1-(1-m 25654 000562-74-3 87 ethylethyl)- 11 8.40 1.19 D:DatabaseNIST02.L 5-Dodecene, (E)- 34726 007206-16-8 25 Heptafluorobutyric acid,n-tridecyl 155417 1000216-78-9 25 ester Heptafluorobutyric acid, n-tetrade 158317 007365-36-8 25 cyl ester 12 9.16 2.38 D:DatabaseNIST02.L 1,3-Benzodioxole, 5-(1-propenyl)- 30379 000120-58-1 94 1,3-Benzodioxole, 5-(1-propenyl)- 30382 000120-58-1 94 1,3-Benzodioxole, 5-(2-propenyl)- 30384 000094-59-7 93 13 10.22 1.18 D:DatabaseNIST02.L Decane 18419 000124-18-5 30 1,4-Benzenediamine, N,N'-diethyl- 31788 003010-30-8 30 Benzenemethanol, 4-(1,1-dimethylet 31873 000877-65-6 30 hyl)- 14 10.69 15.58 D:DatabaseNIST02.L 1,3-Benzodioxole, 4-methoxy-6-(2-p 50186 000607-91-0 96 ropenyl)- 1,3-Benzodioxole, 4-methoxy-6-(2-p 50187 000607-91-0 96 ropenyl)- 1,3-Benzodioxole, 4-methoxy-6-(2-p 50188 000607-91-0 90 ropenyl)- 15 11.10 1.15 D:DatabaseNIST02.L Cyclopropane, 1-butyl-2-pentyl-, t 34843 074663-87-9 42 rans- 4-Heptafluorobutyryloxyhexadecane 162900 1000282-97-2 38 5-Undecene, (E)- 25825 000764-97-6 38 I hope you can all make sense of that. Please people note that % in this case relates to a percentage of a particular peak means the percentage of the total area under the total ion chromatogram (TIC) not the percentage makeup of the oil in % mass. Anyone who wants to view the raw data is welcome to email me and I'll email you the raw data file so you can scan the data files with your own personal library. There are definitely a few peaks in there that don't match anything on the standard library. Check out the low match scores out of 100. So if you have any ideas what these unknowns might be you could run some standards and add the mass spectra to your library. I've discovered much to my disappointment that NIST02 doesn't contain croweacin. However, it does have croweacic acid. I think croweacin matches myristicin to some degree. We might get a strong hit to croweacic acid if we pre-oxidise our oil with permanganate...
  10. Fractalhead

    Monogamy - what is it good for?

    So how do people feel about remarriage after a partner is deceased? As much as I would like my wife to be have a happy life, I have to admit that on one level, if I died I would be really seriously rolling in my grave if I found out that some lucky living bastard was screwing my wife while I was on the other side. Also, those of you who are believers in monogamy, what is the longest period of time you have been physically separated from your darling? After my wife and I fell in love (we'd been together for about 4 months) we agreed to commit to one another and spend the rest of our lives together. Then shortly after this we parted (physical) ways and she stayed in qld while I moved to perth to start a uni course. We had agreed that she would come over to perth for a holiday (at least initially only a holiday) one year later. I would have to say that this was probably the most depressing period of my life so far. She ended up coming over in six months and let me tell you, I thought a LOT about love, fidelity and trust during that six months. With a LOT of love, trust, admiration, respect, confidence in the future of our relationship... and well lets face it, a little help from my hand, I stayed true to my wife. However, I think the most important inspiration for staying on track was a deep sense of spiritual connection with her such that I knew we were meant to be together and that there were very special things in store for us if we were smart enough to put the effort in and stay together long enough to find out what they were. I think that this sense of connection is due at least in part to a series of rather full-on coincidences and symbols that happened/appeared to each other in visions before we had met one another and then immediately recognised (much to our shock) after talking to one another about our experiences prior to finding each other. These shared visions occured both on psychedelics and while straight and awake as well as during dreams. I remember we had a strong sense of "everything else has been leading to this". Now I see our relationship like an thrilling novel where a new exciting spirituo-sexual twist could happen at any moment and I just can't bear to put the book down
  11. Fractalhead

    SAB email deleted

    Hey Horatio, Did you remember to type your complete username (ie. [email protected]) rather than just the nick 'xxxxx'? I almost fell into this trap when trying to log onto the new system on someone elses computer. Now I can't get the login page to load up on my computer which is running IE 6.0.299. I can get past that first little pop-up by clicking OK but then I get the message: "ERROR: requested URL could not be retrieved" in the title bar and "
  12. I just logged onto the new email system to discover a couple of strange emails from [email protected] and [email protected] telling me for two different reasons to open up a 42k .zip attachment file. I'm very suspicious of these emails and as much as I don't want to lose my SAB email account, I don't want to unwittingly invite viruses into my system. Are these emails for real?? While we're on the topic of spam, is there any way to block all this crap that is flooding my SAB account? Here's the first one: Subject: Email Account Suspension From: [email protected] Date: Wed, June 29, 2005 7:05 pm To: [email protected] Priority: Normal Options: View Full Header | View Printable Version Dear user fractal, It has come to our attention that your Shaman-australis User Profile ( x ) records are out of date. For further details see the attached document. Thank you for using Shaman-australis! The Shaman-australis Support Team and the second one: Subject: IMPORTANT NOTIFICATION From: [email protected] Date: Thu, June 30, 2005 2:04 pm To: [email protected] Priority: Normal Options: View Full Header | View Printable Version Dear Shaman-australis Member, Your e-mail account was used to send a huge amount of unsolicited spam messages during the recent week. If you could please take 5-10 minutes out of your online experience and confirm the attached document so you will not run into any future problems with the online service. If you choose to ignore our request, you leave us no choice but to cancel your membership. Virtually yours, The Shaman-australis Support Team +++ Attachment: No Virus found +++ Shaman-australis Antivirus - www.shaman-australis.com Download this as a file Attachments: document.zip 42 k [ application/octet-stream ] +++ Attachment: No Virus (Clean) +++ Shaman-australis Antivirus - www.shaman-australis.com
  13. Fractalhead

    Who wants to get into TLC?

    Firstly, even when different experimenters try to use the same solvent system in different laboratories, absolute Rf values can be quite variable since it is difficult to reproduce all the other factors that affect absolute Rf such as condition of the plates (eg. level of dryness), saturation level of the atmosphere inside the development chamber, temperature etc. Even when measures have been taken to control these parameters, absolute Rfs can be quite variable. What is usually more helpful is looking at the relative distances travelled by different spots and comparing these to the relative values of Rf reported in the literature in which many standards have been run alongside one another in the same lab under exactly the same conditions. This info can help you pinpoint the compound of interest in a complex plant extract so that you can then use that extract as a kind of standard to look for the same compound in a completely different mixture coming from another plant. This approach is easiest when the typical pattern of spots obtained from a given species has been well mapped and validated with the use of standards (eg. HBWR or P. harmala) but more difficult when no such map is available and you can only get a single Rf for the compound without much to compare it to.
  14. Fractalhead

    Who wants to get into TLC?

    BTW... I can definitely put you together a kit if you want one. Just email me to line something up.
  15. Fractalhead

    Who wants to get into TLC?

    Thats an excellent idea foolsbreath and something I have been trying to get around to for some time now. Which standards do you have in mind? Maybe we should start something along the lines of the Australasian Free Standard Ring (AFStR)... I certainly don'e have time to prepare every standard people might like. These standards don't have to be pure compounds or solutions of pure compounds but may be well characterised mixtures. As long as we have some kind of benchmark. Looks like I need to start offering some small volumetric flasks and some volumetric capillaries... There are a few challenges with this standards based approach however. Firstly is the instability of many of the cooler compounds. Secondly is the legality of many of the cooler compounds. These things make to trade or possession of some standards a little tricky. In these cases, we may have little choice but to use relative Rfs and colour reactions for identification.However these problems only cover the minority of potential standards. Bring it on... I will offer a few standards as soon as I can - GC/MS verified where possible
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