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Found 5 results

  1. I have an old Gelman Sciences Australia Laminar Flow cabinet class I. Model CF43S From the serial # it looks like it was made in 1988. Fuck it's been a corker of a unit. If you're purchasing one new, or even secondhand, I'd recommend them. Of course Gelman Sciences Australia no longer exists the way it did, the company still exists as Gelaire. I have yet to contact them to see if they have any info on the model, but from experience with other equipment the chances of any documentation from that era are fuckall. Last few months the fan has started to make bearing whine noises- especially when it's hot ( I don't have aircon in the lab right now, dammit ). Too broke to bring in a specific technician to replace the fan and test the filter. I'd like to at least find somewhere to buy a similar unit for cheap and install it myself, or to compare the cost of me replacing it with a non-genuine part against the cost of getting a tech down here ( incl. travel time ) to do the whole thing and under warranty I've looked online but can't find a manual, unsurprising since 1988 wasn't a great year for online PDFs ;) Anyone here working with these, or similar units who could advise? Would it be just a case of looking at the diameter of the fan and picking something off Alibaba? Brands to avoid? Anything else I need to know? Took the pre-filter off, it looks to be bolt-on. It could well be a standard blower fan and I might be able to buy something cheap and slot it in the old unit's place. As quickly as possible and on a cool day so as to prevent anything chunky from getting into the airstream/ the seals from warping. The only way to make sure it's still a closed system would be to do the old waft-with-a-stick-of-incense and to check it with some open petri dishes for 30s, 1 min, 3 min etc. And panic if I warped the seals during replacement and then fork out for a technician to visit There's a compliance and info plate which can't be seen unless I fully pull the top steel plate off the unit. If I can avoid that, I will. That shit never ends well when you're working alone and it's mission critical How realistic is this whole scenario? I know a few here have had problems with the airstream in smaller portable units when seals warp or shift and allow pathogens to find a place to live Have pics, put em up later Happy birthday to my beautiful Gelman Sciences Australia Horizontal Laminar Flow Cabinet. 30 years, 20 of them with me :D
  2. Just thought I'd let you know: I have had a 100% sterile success rate with the peroxide agar kitchen tek, both with the cooked method ( no autoclaving ) and the autoclaved method. Methods and comparison below Dispensing and media transfer was done under absolutely not sterile conditions, in order to see if the tek could be replicated anywhere ( those of you who know my kitchen can stop laughing ). I was a tad sloppy with my transfer tek as well to see what would happen Reporting it here because sometimes the signal/noise ratio can be a little loud elsewhere Batch 1- autoclaved + 6ml/L 3% H2O2 Chinese sauce containers were autoclaved in a bag for 20 min 100ml liquid MEA autoclaved for 20 min, consisting of the following 20g/L light malt extract 0.1g/L garden lime 0.1g/L potassium phosphate dibasic 15g/L gelcarin ( 20g/L agar works just as well ) Media was not subject to a pH test Post autoclave the media was allowed to cool and just above setting point 6ml/L 3% H2O2 was added. Media was swirled heavily so that the inner surfaces of the bottle were fully coated Dispensed immediately into sauce containers *on the kitchen bench* in the open air Lids were placed lightly over the containers and 1hr later the plates were completely sealed after inoculation with various species Batch 2- cooked 1hr + 6ml/L 3% H2O2 Chinese sauce containers were autoclaved in a bag for 20 min 100ml liquid MEA cooked for 1hr by placing media container in an open saucepan. Media container lids were left loose. Water came up the the media level- bottles weren't more than 3/4 immersed. Cooked at a slow boil for 1hr 20g/L light malt extract 0.1g/L garden lime 0.1g/L potassium phosphate dibasic 15g/L gelcarin ( 20g/L agar works just as well ) Media was not subject to a pH test Post autoclave the media was allowed to cool and just above setting point 6ml/L 3% H2O2 was added. Media was swirled heavily so that the inner surfaces of the bottle were fully coated Dispensed immediately into sauce containers *on the kitchen bench* in the open air Lids were placed lightly over the containers and 1hr later the plates were completely sealed after inoculation with various species Sterile ( non-peroxide MEA ) library cultures were opened and haphazardly used ( left open for much of the inoculation process ) to inoculate the plates above using a scalpel blade which was only cleaned and flamed between species The following species were placed in the centre of the agar of each container Reishi ( Ganoderma lucida ) Blue Oyster ( Pleurotus spp ) Lion's mane ( Hericium erinaceus ) Elm Oyster ( Hypsizgus ulmanarius ) Plates were sealed with Austraseal and incubated in the dark at 22C 2 plates from the cooked group and 2 plates from the autoclaved group were left uninoculated as controls to check for contamination during dispensing By my judgement it was all a bit haphazard and I didn't believe it would work. Even a contam rate of 10% per batch would have been acceptable At +1 week there is no contamination, anywhere, and growth is good for the Reishi and Elm Oyster. Still waiting on the Blue Oyster and Lion's Mane, but plenty of time yet- those parent cultures could have been a little old- I have storage/ library cultures and can reinoculate from them easily at +3 weeks If you are thinking about the peroxide tek for agar- give it a go. I've only made it sound complex cos I wanted to write it all up so you know I took all the steps. I now pronounce this part of the tek piss easy Edited very fast, because I am an idiot and forgot to put the decimal point in
  3. Hello, I've been doing a lot of research and would love to get any feedback on my first attempt at a Mitragyna Speciosa tissue culture (which is legal plant/tree in my location). I know there are a few threads on here that have addressed this (shonman, Darklight), and I'm not sure if I should post on there and resurrect, or just start a new topic/thread; don't want to hijack shonman's thread, so I'm starting this as a new thread. I appreciate the time, energy, and resources that the members here have put into their practice, and would love to politely pick their brains; I understand that experience was hard-won, so I don't want to offend and ask for "info-freebies". My first hurdle is that I have limited mother plant material, and would really like to have at least one successful replication before I run out of plant. I've been working on this for a few months and have not had one yet; many were lost to contamination. A little about my method: -Working under plastic bin hood - WPM at label suggested strength - SIDNC (Milton Tablets) at 5000ppm for 15 minutes, but I wonder if my tissue samples are too large? Anyone tried 300-400ppm for 24 or so hours? -I also mix the SIDNC into the medium at 0.032%, but having a hard time adding it at the right time (ie. low enough temp to keep avail chlorine) without the medium ending up a little too soft. It's almost as if I need to heat/microwave the medium again, but that would destroy the SIDNC protection, right? For medium prep via microwave, pre SIDNC, how long do you let it boil? - 10% v/v coconut water - 4% Honey - 0.5 mg/l TDZ (this strength was at suggestion of fellow practitioner, open to suggestions on strength or using it at all?) Best way to dissolve TDZ powder? I've heard that petioles are best with this species (can anyone confirm that; goal is micropropagation) (I knocked off a leaf, so I decided to try that also, anyone had success with leaves of this plant?) What's the best way to position the sample on/in the medium? Size and cut of sample? Has anyone been able to micropropagate this with only two phases of medium (#1 medium=produce shoots AND roots, then #2 medium=hardening), OR does it need three phases of medium (#1=shoots, #2=roots, #3=hardening), OR four phases of medium (#1=callus, #2=shoots, #3=roots, #4=hardening) OR different combo...? Any thoughts on hormone use for these phases, I know 2,4 D is good for this particular plant to produce callus, but callus is not my end goal, Im not sure how to get callus to reorganize to shoots & roots. Air supply (hole in lid), I've read both nays and yays as to whether or not it needs it, right now, no holes, too worried about dust mites, etc getting in. I saw a thread post on here from a few years ago saying there might be an article/paper/protocol put out or published on this by Darklight and a few others, did that ever happen? I searched and searched, but could not find anything; I wish there were a manual for this;) After jumping in and doing a few trial runs, I can deeply appreciate how challenging and rewarding this can be. I wholeheartedly appreciate any and all feedback and tips! And I hope I've followed proper protocol for rules and etiquette on this post! I normally keep to myself, but after lack of success I realized I needed to reach out for help on this one. I only have pics of my most recent batch. The last one (#5) must be contaminated, can anyone "Name That Contam"?
  4. Distracted

    Sterilitily and grafting

    Hi! So i've done about 20 loph to san pedro grafts in the last month, have used the same blade for everything and haven't sterilized it once. I haven't noticed any bacterial or fungal infections. Any thoughts?
  5. On the podium are five sterile culture containers of Acacia acuminata. Tubes to be auctioned separately. These are aseptically germinated seed, and are suitable for further sterile work, or for deflasking into a suitable environment for garden growth. Once you receive them they are yours to do with as you wish Funds from this auction will be donated to the Seed Saver's Network, to continue their work teaching and mentoring communities in Australia and overseas, encouraging groups to maintain their local, biodiverse and sustainable food resources. Jude and Michel do some of the best outreach work I have ever seen and they have inspired me for several decades now http://www.seedsavers.net/ Auction notes: if you are a successful bidder you accept the terms and recommendations below Plants are approx 2-4cm tall with two explants per jar. They will have another few weeks of growth in them under fresh coolwhite fluro tubes, or indirect light about 200-250 µmol photons/m2/second ( ie about 10-20% of daylight strength- more could cook them ). If plantlets get taller than 7-8cm it can become difficult to remove and handle them aseptically. Auction open to addresses within Australia only, with the exception of WA. I will not ship to WA for quarantine reasons. Cultures are functionally sterile at the time of shipping, but media contains PPM Culture tubes are 150ml, with 50ml culture media containing 10g/L gelcarin. The high gelling agent amount will help, but not entirely prevent root and stem damage during shipping I won't be held accountable for their success in further culture as that relies on factors outside my control. Simple aseptic teks have previously worked for minimal replication of this species. If you post your tek and pics to SAB myself or someone else may be able to help, but that depends on time available to me and clarity of your requests Auction bid includes shipping cost Auction bids open at $20 per jar, and auction closes at midnight on Sunday 28th April 2013 Tube #1 Starting bid $20 Tube #2 Starting bid $20 Tube #3 Starting bid $20 Tube #4 Starting bid $20 Tube #5 Starting bid $20
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