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Showing results for tags 'culture'.
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Mushroom culture confusion
TheMooseZeus posted a topic in MycologyHey all! Im getting into cultivating and am very confused about buying cultures. For example these prices are ridiculous: $150 https://www.newgenerationmushroomsupplies.com.au/index.php?route=product/product&path=101_120&product_id=229 This in the middle: $40 https://forestfungi.com.au/collections/agar/products/white-oyster-petri-dish Compared to this which is very cheap: $15 https://www.miltonmushrooms.com.au/store/p10/White_Oyster_culture_(Pleurotus_florida).html Am i missing something? Where do you recommend i look for mushroom cultures in Aus? - Cheers all
Alcohol, bars and drinking culture...
TheMooseZeus posted a topic in Chill SpaceAustralia is pretty well known for it's drinking culture. Alcohol is a weird one too. The world health organisation recently changed the "Safe" dosage of alcohol from 3 standard drinks to 0... It's pretty well known for it's poison to both the biological body, the mind and society. Bars are interesting - Take the alcohol out of the equation and there's not a lot left. making bars by nature a toxic environment. A friend recently pointed out that if a berry had the same effect as a high dosage of alcohol - The plant would immediately be classified toxic and no one would bother with it again... This to be fair isn't enough to stop her from drinking. I know many people who detest alcohol for it's negative qualities yet in a social situation fall back on it. I've become increasingly confused as to where the appeal in "going out Friday nights" lies, in the social environment or the substance. I occasionally have a few drinks to make things bearable when around others in such a lowered state of consciousness and it sure works a treat. I am able to join in on half assed conversation surrounding and praising the ego. More importantly - what are your thoughts on the topic? (This comes from a point of fear of becoming an outcast in society. This fear further proving how huge of an impact alcohol has on us as a society)
Dissociating from your culture
TheMooseZeus posted a topic in Creativity, Spirituality & PhilosophyI feel the great importance of dissociating from your culture during these early stages of my journey. My culture remains a mould and a restraint from what I want to get out of life. The few experiences I have fulfilling this disassociation have been profoundly freeing yet fleeting and unsustainable. For many (not all) young people this inherited trait shapes the individual for worse not better. Are there any practises anyone has undergone to help free themselves from this?
Your friend in the frudge
rottenjonny posted a topic in MycologyI'm happy today because ay the end of summer last year I threw all the plates I was working on into the fridge and forgot about them. Couple of days ago I took a transfer and they've taken off! Bring on the warmer weather. Now who wants to send me some culture? Keen to grow some massive oysters over the next few months.
Fresh Spirulina starter culture
Kenjo posted a topic in WantedHi Folks Does anyone cultivate Spirulina platensis or know someone that does. I am keen to get hold of about 50 - 100 mls to start my own culture. I would be willing to pay for it.
Anyone got a clean, confirmed Trametes versicolor agar culture
Darklight posted a topic in MycologyLike it says in the title... Seeking clean slant or plate of Trametes versicolor that has been confirmed by fruiting and is ready to send. Will pay. Dammit I knew I shoulda got one isolated from here back when it was raining enough to make mushrooms :D
Darklight posted a topic in MycologyA bloke from Lismore gave me some boletes he described as Slippery Jacks yesterday. No confirm ID yet, and I'm not going to bother really, he's a lifelong forager and has been eating them from this patch for years. I'm satisfied they're edible Have done a lot of searching, and I remember reading the other day someone has managed to cultivate bolete-type mushrooms in beds or bags without associating them with a living tree. It was a recent tek, but for the life of me I can't find it, and it could be just my bad memory Anyone know of or has tried successful cultivation of boletes? I want to put this one on some wood pellet agar and/ or PDA + chloramphenicol for spawn, but if it's not a goer til a tek is perfected I won't take it further
Charity auction: sterile Acacia acuminata in tissue culture
Darklight posted a topic in Seed & Plant SwapsOn the podium are five sterile culture containers of Acacia acuminata. Tubes to be auctioned separately. These are aseptically germinated seed, and are suitable for further sterile work, or for deflasking into a suitable environment for garden growth. Once you receive them they are yours to do with as you wish Funds from this auction will be donated to the Seed Saver's Network, to continue their work teaching and mentoring communities in Australia and overseas, encouraging groups to maintain their local, biodiverse and sustainable food resources. Jude and Michel do some of the best outreach work I have ever seen and they have inspired me for several decades now http://www.seedsavers.net/ Auction notes: if you are a successful bidder you accept the terms and recommendations below Plants are approx 2-4cm tall with two explants per jar. They will have another few weeks of growth in them under fresh coolwhite fluro tubes, or indirect light about 200-250 µmol photons/m2/second ( ie about 10-20% of daylight strength- more could cook them ). If plantlets get taller than 7-8cm it can become difficult to remove and handle them aseptically. Auction open to addresses within Australia only, with the exception of WA. I will not ship to WA for quarantine reasons. Cultures are functionally sterile at the time of shipping, but media contains PPM Culture tubes are 150ml, with 50ml culture media containing 10g/L gelcarin. The high gelling agent amount will help, but not entirely prevent root and stem damage during shipping I won't be held accountable for their success in further culture as that relies on factors outside my control. Simple aseptic teks have previously worked for minimal replication of this species. If you post your tek and pics to SAB myself or someone else may be able to help, but that depends on time available to me and clarity of your requests Auction bid includes shipping cost Auction bids open at $20 per jar, and auction closes at midnight on Sunday 28th April 2013 Tube #1 Starting bid $20 Tube #2 Starting bid $20 Tube #3 Starting bid $20 Tube #4 Starting bid $20 Tube #5 Starting bid $20
Proposed workshop-Sterile Tek for Mycology, Koda, Byron, maybe
Darklight posted a topic in MycologyI've had a few requests lately in-person for help and advice with sterile techniques- usually related to mycology, but also with regard to plant tissue culture It's hard to teach this online, and a lot dispiriting watching so many people burn out over the years trying to learn these teks in isolation I'm thinking about running a Sterile Tek for Mycology workshop to be run at Koda Phytorium in Byron Bay around early June. This will be a 2 day workshop of approx 5 hours a day with a lunch break in the middle I'd love some feedback on the course outline below. What would people like to see? What is redundant? Would you attend if you were in the area? Does it sound interesting for n00bs? Sterile Tek for Mycology Location: Koda Phytorium, Byron Bay Date: Mid June 2013, ( TBA ) 2 days Cost: About $350 per person ( but TBA, it's equipment intensive ), this includes your own take-home perspex tunnel and some hardware The proceeds of the workshop ( all the course fees minus equipment expenses ) will be donated to the Australian Ethnobotanical Association http://www.shaman-australis.com/forum/index.php?showtopic=34208 Limit: 4 people Aim: This workshop provides good practical grounding for culturing and growing gourmet edible and medicinal mushrooms at your home or community group. It is aimed at beginners and enthusiasts We'll be doing practical sessions with four woodloving species ( primary decomposers ): Oyster mushroom ( Pleurotus spp ), Shitake ( Lentinula edodes ). Shimeji ( Hypsizygus tessellatus ) and Reishi ( Ganoderma lucida ) This will be a small, practice-based, lab-centred workshop, covering theory and some practice of sterile tek, and some semi-sterile teks like hydrogen peroxide, shiitake newspaper logs and Pleurotus straw bales You don't need any experience with mycology to attend this workshop. You should be over 18, though we can waive that for people who can show maturity and commitment or have a parent or guardian who will be responsible for you and undertaking the course on the same day. This short practical course is extensive but not exhaustive- we'll cover the basic teks and theory but there are always new teks coming online. If you have some myco experience and would like to attend, we really welcome your input as long as you understand that you may be one of the more advanced students in the class Topics we'll cover: Biology and growth of edible and medicinal species. Ecology. Safety. Sterilisation and aseptic theory and practice. Inoculation and growth on solid and liquid media. Contamination and contam patterns. Spawn and dowel culture and handling. Research. Maintaining your culture library. Record keeping. Storage, and drying. We'll make some shiitake or reishi newspaper logs and a couple of Pleurotus straw bales. You'll take away some culture slants of your own. and a few extra copies we'll supply you with, a reishi or shiitake newspaper log, your own perspex tunnel and hopefully a head full of knowledge All workshop profits ( basically your course fee minus what I pay for the perspex tunnels and supplies ) will go to the Australian Ethnobotanical Association You will need to wear covered shoes. For best results and your own safety please keep long hair tied back during prac sessions. Bring a hat and something to drink for the outdoor sessions. Please make your own food, drink and accommodation arrangements The techniques you learn at this workshop can also be applied to plant tissue culture, and we may run workshops on that in future, however practicing these teks using your perspex tunnel after the myco workshop will significantly increase any benefit you get from a plant tissue culture workshop