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I have some really old Al backed TLC plates I'd like to use up before forking out the dosh for more. Can't find the original order, but they had been snipped up and used for alkaloids a coupla decades ago so I'm assuming they had fluorescence inbuilt There was an old thread here that mentioned that old plates were still viable if they were re-treated and dried in the oven. Google and site search here aren't being useful. SAB site search shows nothing, and Google is throwing me too much irrelevant data Anyone know the protocol or has a link to a relevant page? cheers people
Darklight posted a topic in Chill SpaceI have an old Gelman Sciences Australia Laminar Flow cabinet class I. Model CF43S From the serial # it looks like it was made in 1988. Fuck it's been a corker of a unit. If you're purchasing one new, or even secondhand, I'd recommend them. Of course Gelman Sciences Australia no longer exists the way it did, the company still exists as Gelaire. I have yet to contact them to see if they have any info on the model, but from experience with other equipment the chances of any documentation from that era are fuckall. Last few months the fan has started to make bearing whine noises- especially when it's hot ( I don't have aircon in the lab right now, dammit ). Too broke to bring in a specific technician to replace the fan and test the filter. I'd like to at least find somewhere to buy a similar unit for cheap and install it myself, or to compare the cost of me replacing it with a non-genuine part against the cost of getting a tech down here ( incl. travel time ) to do the whole thing and under warranty I've looked online but can't find a manual, unsurprising since 1988 wasn't a great year for online PDFs ;) Anyone here working with these, or similar units who could advise? Would it be just a case of looking at the diameter of the fan and picking something off Alibaba? Brands to avoid? Anything else I need to know? Took the pre-filter off, it looks to be bolt-on. It could well be a standard blower fan and I might be able to buy something cheap and slot it in the old unit's place. As quickly as possible and on a cool day so as to prevent anything chunky from getting into the airstream/ the seals from warping. The only way to make sure it's still a closed system would be to do the old waft-with-a-stick-of-incense and to check it with some open petri dishes for 30s, 1 min, 3 min etc. And panic if I warped the seals during replacement and then fork out for a technician to visit There's a compliance and info plate which can't be seen unless I fully pull the top steel plate off the unit. If I can avoid that, I will. That shit never ends well when you're working alone and it's mission critical How realistic is this whole scenario? I know a few here have had problems with the airstream in smaller portable units when seals warp or shift and allow pathogens to find a place to live Have pics, put em up later Happy birthday to my beautiful Gelman Sciences Australia Horizontal Laminar Flow Cabinet. 30 years, 20 of them with me :D
(1) For the love of all things good in this world, do not store more grain than you have to; or at least store it properly. For most of my commercial cultures I use organic wheat grain. Some time ago I bought 30kg sack, thinking I would cut down on costs by buying in bulk. The first sign of trouble was a few pantry moths flying around the lab. No problems I thought, I'll leave the sack out in the sun for the day to kill off the weevils and store it in another room. Well, it turns out the sack was infested, and with the humid warm conditions of late summer, I soon had 100's of moths in all my rooms, in all my medias, in all my grains, in all my supplements, in all my everything! (2) Put your agar plates in the fridge as soon as they have set. Leaving agar plates out on the bench for extended periods of time gives mites (YES MITES) a chance to crawl in, contaminate your plates with asper. sp and/ or lay eggs that will ruin your culture later. Don't try to save your plates; freeze them to kill the mites and throw away. Again, this time of year is particularly bad with high humidity. (3) If you can avoid it, don't have any timber in your clean space- given enough time and moisture you can have a mold city growing under benches, which you wont know about, absolutely loading the air with spores. Keep it stainless or plastic. Learn from my mistakes fellow mycologists. With love, Mimz