Jump to content
The Corroboree

Search the Community

Showing results for tags 'kitchen'.

  • Search By Tags

    Type tags separated by commas.
  • Search By Author

Content Type


Forums

  • Knowledge
    • Ethnobotany
    • Cacti & Succulents
    • Australian Native Plants
    • Mycology
    • Pharmacology, Chemistry & Medicine
    • Sustainable Technologies & Ethical Living
    • Members only! Not visible to google and guests.
    • Legal Matters
  • Community
    • News & Notices
    • Chill Space
    • Creativity, Spirituality & Philosophy
    • Seed & Plant Exchange
    • Site & Forum Matters
    • Bitches, gripes and degenerated threads

Blogs

  • Torsten's Blog
  • hebrew's Blog
  • Ed's Blog
  • Archaea ain't not a fool
  • al
  • bloodbob's Blog
  • kakti's Blog
  • Jack's Blog
  • willbe's Blog
  • De Quincey's Blog
  • Auxin's Blog
  • EG´s Trading Blog
  • n00dle's store (selling and wanted)
  • creach's Blog
  • hellofromthegutter
  • Darklight's Blog
  • Rimbaud's Blog
  • naja naja's Blog
  • ipomike82's Blog
  • Tomer Corymbosa's Blog
  • T-A's Blog
  • jasemateau's Blog
  • proprioception
  • LurkiestLurker's Blog
  • wandjina's Blog
  • IndigoSunrise's Blog
  • onz's Blog
  • Gollum's Blog
  • Tripitaka's Blog
  • AndyAmine.'s Blog
  • Bijanto's Blog
  • Frank's DarkPlace
  • min(E)rval's Blog
  • Apprentice of the Cricket Shaman
  • foolsbreath's Bazaar
  • oxydiser's Blok
  • MORG's greenhouse.
  • shane6891's Blog
  • bluemeanie's Bog - I mean Blog!
  • anachronism's blog
  • spudamore's Blog
  • Plant Psyentist's Blog
  • Teo's Patch
  • gomaos' Blog
  • VINS' Blog
  • DreamingNagual's Blog
  • evolutionNth's Blog
  • muchomundos' Blog
  • wisemonkey's Blog
  • The Poet's Blog
  • The Pleasure Dome
  • Merkaba's Blog
  • Yopo, Ololiuhqui, Cola nuts etc...
  • SaBReT00tH's Blog
  • without's Blog
  • reshroomED's Blog
  • Ace's Blog
  • The Kenny Blister Blog
  • PhantomTurkey's Blog
  • Nasty's Blog
  • Auntyjack's Blog
  • Harry's Blog
  • SirLSD's Blog
  • Hagakure's Blog
  • tardistestpilot's Blog
  • Shroomie the Shaman's Blog
  • Gardener's Blog
  • Trade List
  • GreenDreamBlog
  • tonic's Blog
  • blogs_blog_85
  • touchesclouds' Blog
  • My trade list
  • rhiz-o-mat
  • Irrational Dribble
  • ::fires in the dark::
  • spacemonk's Blog
  • Yeti101's Blog
  • Zakmalados' Blog
  • Wiz
  • Hunab Ku's Blog
  • Kenny
  • GREEN HOUSE AKA spice1's Blog
  • planthelper's Blog
  • Looking For:
  • Teotz's Blog
  • rootbark's Blog
  • Flip's Blog
  • Eccentro's have/want list
  • Fritz Haber's Blog
  • Techno and Stuff
  • santiago's Blog
  • Punkin's Blog
  • centipede's tradelist
  • gecko's Blog
  • ref1ect1ons' Blog
  • Desmodium pulchellum
  • lofty86's Blog
  • illegalbrain's Blog
  • Trade List
  • eatfoo's Blog
  • BioMedHed's Blog
  • BioMedHed's Blog
  • qualia's Blog
  • Le Growlogue
  • BioMedHed is online...
  • LetTheTreesGrow
  • quarterflesh's Blog
  • DarkSparks Blog
  • DarkSpark's Blog
  • gazal's Blog
  • Evil Genius' Blog
  • happypills87's Blog
  • noshiewa's Blog
  • juzzoa's Blog
  • omsource's Blog
  • silver462's Blog
  • Ballzac's ramblings
  • Psylo Dread's Offerings
  • 2Deep2Handle's Blog
  • mysubtleascention's Blog
  • mysubtleascention's Blog
  • Soviet Telepathy Experiments
  • Juergen's Blog
  • amanito's Blog
  • bujinkan321's Blog
  • steiner's Blog
  • Pepico's Babies
  • Peru 01
  • bullit's Blog
  • Growblog
  • Photoblog
  • MikeyMagic's Blog
  • NegativeDave's Blog
  • notes from the field
  • News that doesn't deserve a thread
  • I need a CACTUS!!!! (succulents included!!!)
  • TrailBlazer's Trade / Want List
  • Sabry's Blog
  • tonyarcher's Blog
  • The Observer
  • Pappa somni's Blog
  • Pappa somni help? Seeds, info, guidance
  • nikkaoineza88's Blog
  • gr33ntea's Image Misc
  • El presidente Hillbillios' Blog
  • igro's Blog
  • igro's Blog
  • myriostigma
  • AbleKay's Blog
  • Lamps or not.

Find results in...

Find results that contain...


Date Created

  • Start

    End


Last Updated

  • Start

    End


Filter by number of...

Joined

  • Start

    End


Group


AIM


MSN


Website URL


ICQ


Yahoo


Jabber


Skype


Country


Interests


Climate or location

Found 1 result

  1. Just thought I'd let you know: I have had a 100% sterile success rate with the peroxide agar kitchen tek, both with the cooked method ( no autoclaving ) and the autoclaved method. Methods and comparison below Dispensing and media transfer was done under absolutely not sterile conditions, in order to see if the tek could be replicated anywhere ( those of you who know my kitchen can stop laughing ). I was a tad sloppy with my transfer tek as well to see what would happen Reporting it here because sometimes the signal/noise ratio can be a little loud elsewhere Batch 1- autoclaved + 6ml/L 3% H2O2 Chinese sauce containers were autoclaved in a bag for 20 min 100ml liquid MEA autoclaved for 20 min, consisting of the following 20g/L light malt extract0.1g/L garden lime0.1g/L potassium phosphate dibasic15g/L gelcarin ( 20g/L agar works just as well )Media was not subject to a pH test Post autoclave the media was allowed to cool and just above setting point 6ml/L 3% H2O2 was added. Media was swirled heavily so that the inner surfaces of the bottle were fully coated Dispensed immediately into sauce containers *on the kitchen bench* in the open air Lids were placed lightly over the containers and 1hr later the plates were completely sealed after inoculation with various species Batch 2- cooked 1hr + 6ml/L 3% H2O2 Chinese sauce containers were autoclaved in a bag for 20 min 100ml liquid MEA cooked for 1hr by placing media container in an open saucepan. Media container lids were left loose. Water came up the the media level- bottles weren't more than 3/4 immersed. Cooked at a slow boil for 1hr 20g/L light malt extract0.1g/L garden lime0.1g/L potassium phosphate dibasic15g/L gelcarin ( 20g/L agar works just as well )Media was not subject to a pH test Post autoclave the media was allowed to cool and just above setting point 6ml/L 3% H2O2 was added. Media was swirled heavily so that the inner surfaces of the bottle were fully coated Dispensed immediately into sauce containers *on the kitchen bench* in the open air Lids were placed lightly over the containers and 1hr later the plates were completely sealed after inoculation with various species Sterile ( non-peroxide MEA ) library cultures were opened and haphazardly used ( left open for much of the inoculation process ) to inoculate the plates above using a scalpel blade which was only cleaned and flamed between species The following species were placed in the centre of the agar of each container Reishi ( Ganoderma lucida )Blue Oyster ( Pleurotus spp )Lion's mane ( Hericium erinaceus )Elm Oyster ( Hypsizgus ulmanarius )Plates were sealed with Austraseal and incubated in the dark at 22C 2 plates from the cooked group and 2 plates from the autoclaved group were left uninoculated as controls to check for contamination during dispensing By my judgement it was all a bit haphazard and I didn't believe it would work. Even a contam rate of 10% per batch would have been acceptable At +1 week there is no contamination, anywhere, and growth is good for the Reishi and Elm Oyster. Still waiting on the Blue Oyster and Lion's Mane, but plenty of time yet- those parent cultures could have been a little old- I have storage/ library cultures and can reinoculate from them easily at +3 weeks If you are thinking about the peroxide tek for agar- give it a go. I've only made it sound complex cos I wanted to write it all up so you know I took all the steps. I now pronounce this part of the tek piss easy /applications/core/interface/imageproxy/imageproxy.php?img=&key=ed93ee4b8a158835e0af19ead9c794b11af03de360911f7858b9da338588c45d Edited very fast, because I am an idiot and forgot to put the decimal point in
×
×
  • Create New...