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The Corroboree

Darklight

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Everything posted by Darklight

  1. Darklight

    Agar

    Sounds good If you are starting from spores though, remember you can't add hydrogen peroxide to your media. Hydrogen peroxide kills spores
  2. Darklight

    Agar

    K- so you have made what's called water agar It's not really nutritious at all and any growth you get could be resulting from residual nutrients in whatever it was you inoculated it with. That could be why growth is slow A general purpose agar is something like Malt Extract agar There are a million recipes for Malt Extract agar, often just varying the amounts. One that's worked for me for a variety of species is: 20g/L light malt extract ( brewing supply shops sell it ) 100mg/L garden lime ( Bunnings ) 100mg/L potassium dihydrogen orthophosphate ( some people omit this ) 20g/L agar ( health food shops ) A litre of agar is heaps! Like, about 40 standard petri dishes worth. So work out how much you actually need and change amounts accordingly If you want to add 3% hydrogen peroxide at 2-8ml/L, do that after you have cooked your agar and the container is at a temperature which can be held comfortably in your hand Malt Extract Agar is really nutritious. Lots of species like it- so do lots of contaminants. Not all species are happy with you adding hydrogen peroxide, but many will overcome their distaste for it and grow after a couple of weeks Good luck!
  3. Darklight

    Agar

    Hi john Welcome to the wonderful, frustrating, fun world of growing mushrooms The agar question is a helluva lot to unpack. There are a bazillion recipes for 'agar' ( which is really only a generic word for whatever gelling agent is used to solidify the semisolid stuff in a petri dish, but the common use now includes whatever else is in the mix ). Confusing as fuck IMO Most people just use what's convenient for them that works, hence all the different agar types. Some types are specific to species, some are general, some used to deter competitors to get a cleaner culture etc Are you using only agar and water- your OP refers to straight agar- or is there other stuff in there, malt extract, dog food, vegemite, lime etc H2O2 ( hydrogen peroxide ) kills spore of bacteria and fungi without killing most mycelia- up to a point ( it can be overloaded, degrades if you keep the agar plates for too long, and shouldn't be added until your agar has cooled to the point where you can hold the container by hand ) 3% hydrogen peroxide is usually added 2-8ml/L when your agar has cooled and is ready for pouring. It can negate the requirement for pressure cooking your agar and is also handy if you don't have a sterile cabinet or still air box to work in. I've poured H2O2 agar in the open in my kitchen and had good success with it. I usually carry some plates of it with me if I'm taking samples from mushrooms I find on bushwalks You don't need H2O2 added to agar, but there are some situations which it really helps. Some species don't like it much and some take a while to establish on it Hope that clears things up. Good luck!
  4. Darklight

    Whomping Willow Nursery

    Hey Prier- that's awesome news, congratulations! Was really looking forward to seeing you at EGA this year, I've been coveting some of your lovely cacti offerings there for a long time and a catchup with you is always lovely Took me a minute to find your ebay store, but there you are: https://www.ebay.com.au/usr/whompingwillownursery You'll rock it! Coupla decades experience in cactii will serve you well
  5. Sorry to hear about your dad mate, but it's beautiful you think of him so highly My dad's had Parkinsons for a long while, as have a few others I know, I've been following the research and their progress for a while. Unfortunately none of the people I know with Parkinsons are interested in support outside their direct relationship with their GPs ( who mostly don't know much about Parkinsons ) But swimming's one of the best things your dad can do, if he's having trouble can you get him to wear a flotation device? I know there can be a bit of pride about wearing one in a public place... but swimming's so good for stages of PD. In Australia I've seen water exercise used as a recognised form of therapy for PD and it did work for my dad as a part of slowing his Parkinsons progression No, you seem awesome and loving. I wondered about those compounds too back when Dad was diagnosed. I think you're right about potential deficits after use. Am not a physician and there are people here who are better qualified than I to explain the mechanisms There are some biological compounds I'd look at first before directly going for the pure stimulants Mucuna pruriens seed could be a good place to start if he's not already on meds ( buy it in, or only grow the hairless subtype ) CBD is approved in Australia for treatment of some PD symptoms. Not sure about THC, but CBD is definitely on the list and I've heard ( and only heard- not seen yet ) good things of it for this. Not sure about legality/ accessibility where you live. Is most likely to be well tolerated alongside other medications Ibogaine root bark has been reported to reduce symptoms of PD too. There's a bunch of glowing reports on that if you google ( I do not trust glowing reports on Google much, but low dose ibo is awesome and I'd personally give it a go if I ever got a diagnosis) - but if you're recommending it to anyone else- particularly if they're already on other medication- I'd take real care to find a sympathetic primary health care professional and a good clean source of ibogaine root bark Was most interested to hear psilocybin/ mushrooms mentioned above, now I'll have to do some reading :) All the best for your and your family's journey.
  6. Darklight

    fungi and slugs

    Whoa- lovely work, seriously impressive. Definitely warrants further investigation at other sites
  7. Darklight

    Cordyceps Militaris growing

    Thanks for the peptone links lindsay and saguaro, I will get some just for me, it turns up in recipes for other species from time to time
  8. Darklight

    Cordyceps Militaris growing

    Thanks, this is lovely work, well set up and thorough, will follow Where's everyone source their peptone from? I bought some ages back but it was pretty exxy and it seems lots of Cordy teks use a fair bit
  9. I've got an old Olympus ECEBi microscope and I love the damn thing. However it's a little dusty inside, and I'd like to do some spore identification with it, so I need crisp vision at high magnification A few mates have the same base model or a variant thereof. They were super common back in the day Yep, they're from the 70s or something, but they're well made. I love well made things :) Having trouble finding someone who will clean it- not just the outer, but the gubbins inside, which seems dusty Have spoken to a few professionals about it who have said it's a fair bet they're not worth looking at, and the average hourly rate ( including part thereof ) for a professional clean is about $200 an hour- that would also cover the evaluation, even if no work was done Bit lost with what to do about this. I'd also like professional advice on storing the damn thing so it doesn't suffer from fungus, which seems to get into a lot of scopes in NNSW Anyone have actual experience with getting this done? Not just links- but actual experience?
  10. Darklight

    Garden States Microdose - Free livestream with Dennis McKenna

    Even me? Def gunna check out Caine's presentation. Good solid local science Love your work my old friend <3
  11. Darklight

    Killing yellow stainer colony?

    Yep, I wouldn't throw fungicide or glyphosate on it. Ick. Have a crack at throwing a fair whack of Aglime on it. Raising the soil pH might discourage that species and encourage a different one. Whether it's one you like or not is another matter If you have heaps of local used oyster mushroom straw or other local spent mushroom spawn I'd throw that on top of the aglime. I haven't tried this, it's just something that's inline with basic mycological and biological processes. Inhibit the thing you don't want, encourage the thing you do And saguaro is right, there will be spores aplenty, and mushrooms will always come up when conditions are right for them and not their competitors Yellow stainers prolly occupy an important niche, don't wipe em out Let us know how you go
  12. Wow. Just wow. Awesome, thank you so much! I knew nothing of these. What's the yield like for a domestic setup? Either per kg or how many single portion meals or meal additions you'd get per bag? This is now one of my one-day-I'll-get-to-it projects. Glad you got there first :D
  13. Darklight

    Shoutout to the firies

    Well, shit. Worst season I've seen, ever, and I was here for the '93 fires. There's a few crew affected, either directly or in the path thereof. Prolly more than I know. But I've seen such grace on the firegrounds which has pushed past my normal misanthropy, and some of that grace has come from some of you. Shoutout especially to @niggles and @teamwhy, whose continued and protracted presence onsite is making a huge difference. Niggles mate you lost yer fire cherry well and truly this year, you've been doing some long days, done some hard fucken yards and I'm proud of you. Teamwhy, seeing you on comms and knowing you're there day after day makes me smile like a goose. Also whoever to put those tiny sweet garden mandarins in the snack packs the other day, they were small and hard won in the drought and sweet as fuck and much appreciated Shoutout to the civvies at Tuntable and Siddha Farm feeding everyone and pushing firebreaks through impossible country and the yoof being all splendid rolling round on ATVs, everyone's hearts swell just seeing them rock a fantastic and major contribution. Anyone who thinks Millennials are snowflakes should see this lot, they're pure gold. Shoutout to the firies everywhere. Everywhere I go I see trucks from somewhere else. Shoutout to Mudgee firies who came up to help out at Drake, now they've got their own shit going down locally like a few other brigades who went well out of area up here. And the CFA ppl in VIC, I think we have a couple on here And none of this Hero Firies shit. Firies are you. This hero worship thing just separates the people who do the work from too many people who can, but aren't. Join up, help out, at the very least clean your fucking gutters, talk to your neighbours and get your evac plan in line ( yes, I do understand that not everyone can kick in and the entry requirements are onerous these days, that's for another thread ) You should see who's out there, it's amazing. The fires are fucked and the environmental devastation is heartbreaking. The humanity so far is splendid Pray for rain. Dance harder, fuckit
  14. That. Was. Beautiful. Thanks to Ronny, Obtuse and Adele and the EGA crew for putting this together. Was very much worth the trip to Melbs. Panel was gorgeous, hell, the audience was gorgeous. A lovely mixed bunch of age and backgrounds, some were familiar with EGA's work, others knew more about the myco-side but quite a few knew of both. Audience had a pretty good grounding in all things mushroomy, which made for some excellent questions to the panel. The book table near the ticket point was often crowded with people who had at least one of the references if not more before they even got there. The venue was awesome The panel. I am such a myco-groupie. They all made themselves available for chats before the event and during intermission and a few stuck round afterwards and they were all fucken lovely people with a grace I can only envy. Question time was professional, comprehensive and respectful and thus also awesome If you get a chance, go to one of Dr Alison Pouliot's workshops or forays. She's got a really impressive schedule the next few months, quite a few covering specific species of interest ( how cool's that ) and apparently they book up full- fast. https://alisonpouliot.com/workshops-events/ Sapphire and Fungi4Land: https://fungi4land.com/ about the most worthy book project of the decade. Love her work. I will buy all the t-shirts. Fungimap and iNaturalist. Best citizen science work on the entire planet ( I will find you that reference Cam, I promise ) Do they even need a link? Everyone knows em. Here, have the link anyhow https://www.inaturalist.org/projects/fungimap-australia Ah yeah, the film. Didn't get to see it. Sure it was fantastic tho, everyone seemed to like it :D Kudos to you all the lot of you. And thanks again Bonus points to me for being lucky enough to hang out with Obtuse heaps, and for working out how to use the bloody coffee machine thing at the flat
  15. Righto, I bought tix to the second screening, just on the off chance I can get down there. Ends 2300 The first, early screening is already sold out. I so wanna be at the panel thing, congratulations and thank you for getting all those people together Obtuse and Ronny and EGA crew. Hope to see you there
  16. Darklight

    Wasabi wanted

    From seed? Easy. A great beginner's project, really worth a go. Sow a lot, some in individual containers, some with a few per container. Split the batch in half and put one of the halves in the fridge for 3-4 weeks then return it to your light rack. If you wanted to start from a live plant it could be more challenging. Start with seed in tissue culture and let us know how you go
  17. Darklight

    Shrooms become a bit more magical

    Handy- thank you!
  18. Ermahgord. I just saw this. I am very seriously thinking about heading down to Melbs for the night on the off chance I get to even be in the same room as the panel crew, all of whom are my idols
  19. Darklight

    Wasabi wanted

    i sent some down to ReShroomed a few years ago ( mmm, maybe a decade now I think about it ). TC clones of seed grown. They grew down at Colac like corkers and the leaf size was 6x what I got in NNSW within weeks ReShroomed passed away a few years ago :( No chance of getting plants out of him now either... allrareherbs.com.au site says they're delivering Wasabia offsets in April But try the seed too. Even if you don't end up with a commercial-grade wasabi root, the leaves are great for winter salads and the stalks apparently are useful too Give it a go I reckon
  20. Erg, I will pour those plates in the morning, so disorganised here :/
  21. Darklight

    Shoutout to the firies

    It is all so unbelievably sad I'm short of words. I hope you stay OK, and I hope it rains
  22. Darklight

    Shoutout to the firies

    Ooh er, thank you, I'll SMS you. I fell asleep and didn't do those plates yet, but they won't take long tomorrow I can't wait, this'll be awesome if we get it
  23. Nice Is that the inside of the pseudo-sclerotia or the fruit? I'm guessing the former. If so, it doesn't look very appetising, might be a good one to inoculate onto seedlings for re-veg work What are you up to tomorrow? I'll pour these plates out tonight. And try not to pour too many different agar types, we all know how that ends up If the inside of the fruit looks easier to culture and breaks up/ cuts well I'd love to grab a fresh fruit from either you or Niggles or both I'll bring some fresh H2O2 MEA plates for you both too. Best to have 'em fresh before the H2O2 goes off. Never know what you'll find in a hot forest
  24. Darklight

    Shoutout to the firies

    Is alright, I knew what you meant and I was into using the wrong term as well. Am most interested in getting a tissue sample onto fresh H2O2 plates ASAP. Cancelled all other appointments today, making plates now. Niggles is hunting samples as well. What are the chances of me getting some tissue samples off you late sarvie/ do you want me to bring over some fresh H2O2 plates? What tree species are the pseudo-sclerotia originating from? I might have to make up some woodchip media for it I'm so easily distracted....
  25. Darklight

    Shoutout to the firies

    You reckon they're the same as Niggles is finding? Check thread http://www.shaman-australis.com/forum/index.php?/topic/46677-found-growing-in-the-ash-from-still-burning-bushfires/ WHAT? THERE ARE TRUFFLES? YOU'RE FINDING TRUFFLES? @teamwhy Get tissue samples and dry em proper, at least 200mg. I'll add them to the collection of things that need sequencing ( special kudos to Obtuse, who explained at length why this should be done properly ) Put some fresh tissue down on H2O2 MEA agar plates. If you don't have any I will make some of the bastards up. Niggles might have access to some too, but they will be a coupla months old *sigh* you prolly don't have time, but if we can grab some properly identified edible native truffles we will all be well stoked, and we can try to add some to any regen work. Anyone else got any favourite firies out there?
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