yellow oysters produceing asexual spores

[lindsay]

purchased some yellow and pink oysters on dried grain. so I drop a gain on a few h2o2 agar plates and they covered the 90mm dishes in three days. well this didn't surprise me, I've grow other oysters before.

but this did.

I have read that some oyster strains produce asexual spores. my guess is this is what I am looking at.

my question is, is this a problem off any sort. I am looking to, putting into slants for later growing and

putting on grain for growing now. can I stop this from happening.

[tripsis]

Nope, you're looking at mould there.

[Bigred]

Have you done all your work in a aseptic manner . ie like a glove box

[lindsay]

all my work is done in front of flow hood.

I still not shore of it being mould.

done both pink and yellow oysters on the same

batch of agar and strait after each other. "but" on a separate sealed pack of dishes (this may be the vector)

the pink are pure white, but the yellow plates all look the same.

they have this evenly spread of black spots siting in/on white cotton.

'sorry' I don't believe it is mould, but yes I may be wrong.

page 73 stamets ggmm

Mycelium of Pleurotus cystidiosus and

allies, species possessing both sexual and asexual

life cycles. Black droplet structures contain hundreds

of spores.

there is a pic that goes with this.

then on pg 74

In culture, these forms appear as

"contaminants," confusing many cultivators. An

excellent example is the Abalone Mushroom,

Pleurotus cystidiosus and allies. (See Figure 57).

shit am infringing copy R

if it was the dishes, hell they all were full of spores, bad bad bad batch.

at this stage I appreciate any comments.

[tripsis]

Believe what you want. Tell me, why is it you think mould can't produce even growth?

Pleurotus cystidiosus has larger conidia than what's in your photo, with each conidium composed of a droplet of black liquid. It's impossible to tell from that photo, but are those black point liquid?

[Bert&Ernie]

Looks like mold to me!

also spores dont come out of mycelium right?

Edited November 27, 2013 by jwerta

[Bigred]

Could you take a picture of it on a piece of paper as the granite makes it hard to tell ?

[Zen Peddler]

I've seen all kinds of mold in my twelve years a' growin'. Seen that one a few times. Yellow and pink oysters taste foul as well

[Zen Peddler]

Colour of dish looks grey with little black pin pricks. Its a mold sonny.

interesting thing is the classic pearl oyster which is the only mushroom I've seen in a dish of 100% mold and bacteria simply run over the top of it and kill it.

if I was going to play Petri dish wars id definitely want it on my side!

[Bert&Ernie]

if I was going to play Petri dish wars id definitely want it on my side!

That sounds like a fun game!

[lindsay]

looks like the verdict is mould. cheers all.

just need to hunt down the source of contam.

going to try some more dishes, but I am starting

to think its the grain spawn because it grew with the mycelium.

have gone so long without any contam that I have little experience in identifying it.

I've seen it in the grow room but not in culture.

nothing better for experience than things going wrong in this case.

and everyone telling me the same thing.

now I will need to give the my huge clean room a good clean

[lindsay]

something strange has happened. something I am not understanding.

as you can see from the contam h202 dish at top I have taken some small

peaces that I put on non-h2o2 agar.

this what they look like 2 days later?

you can still see the spots on the transfer in the middle.

why has the mould taken off on the h2o2 plate and not on the non-h2o2.

[Zen Peddler]

I encounter contamination almost constantly but I work with mainly wild prints and cloned isolates.

As you know h202 stops mold spores from germinating - and mushroom spores for that matter- but it just slows down germinated contaminants already present.here the mold was present on the sector added to this plate so the h202 did nothing.

my advice - discard the whole thing and try again again making sure all the vectors are reconsidered.

good luck.

[waterboy 2.0]

flowhood is not the trick to preventing contamination....aseptic technique is

IMO your set up has too much headroom in it above the flow....maybe causing turbulent flow above it.

EDIT - I reckon the grains your problem

Edited November 28, 2013 by waterboy

[altenate]

Def mould.

But in your breaking bad setup is that a rug on the floor??

If so burn that thing now! Are you sterilizing the plates long enough b4 use? & checked your flow hood? Try clean plates of agar, exposed to the flow& see what happens. Also what's your agar mix? That could be part of it.... Sometimes

Having OCD helps with agar.,,,

[worowa]

I reckon it's that crap dried spawn from Bunnings, Mr. Fothergills? I tried some, same shit.

[lindsay]

I reckon it's that crap dried spawn from Bunnings, Mr. Fothergills? I tried some, same shit.

yep your right. but not from bunnings.

[lindsay]

flowhood is not the trick to preventing contamination....aseptic technique is

IMO your set up has too much headroom in it above the flow....maybe causing turbulent flow above it.

EDIT - I reckon the grains your problem

the grains are problem.

cleanroom works well

[tripsis]

I reckon it's that crap dried spawn from Bunnings, Mr. Fothergills? I tried some, same shit.

Bunnings sells spawn?

[worowa]

Bunnings sells spawn?

Apparently they sell mold spawn

It's dried, possibly from UK-shiitake, pink, yellow and whites from memory. They try and flog a particle board with it. I guess when it all fails, it's a case of "too late now, you made a mistake"...

[tripsis]

Wow, that's at least at little bit ridiculous. What's the particle board for?

[tripsis]

Lindsay, plenty of people around here have good quality cultures of a great range of edible mushrooms. I recommend you start from one of these cultures. You will have much better results.

[Zen Peddler]

I've got a great king oyster strain here I started from a store bought clone. Senecense my arse!

[lindsay]

Def mould.

But in your breaking bad setup is that a rug on the floor??

If so burn that thing now! Are you sterilizing the plates long enough b4 use? & checked your flow hood? Try clean plates of agar, exposed to the flow& see what happens. Also what's your agar mix? That could be part of it.... Sometimes

Having OCD helps with agar.,,,

yes love to burn the carpet, but I rent.

had the carpet steam clean before I set the room up.

I lined the floor with builders plastic with a board under

were the chair goes and taped it in to seal the chamber.

the little white box has a hepa filter for vacuum cleaner

with a small fan so I done suffocate or get wacked

from isopropyl. I clean the chamber with bleach, before use each time

and spray the room out before use.

I buy sealed pre-sterilized plates.

have several times left a agar plate in front of the flow hood. all good there.

but its time I do it again.

my agar mix is malt agar and yeast for oysters.

what is OCD?

I am 99% its the grain I purchased was problem.

[Bert&Ernie]

OCD = Obsessive Compulsive Disorder