ferret Posted June 6, 2010 Since I will have a week or so of spare time next week, I'm planning on taking some microscopic pics focussing on the cheilocystidia of a few bluing Gym's and their close allies, collected from around Melbourne, WA and NSW. If anybody I haven't contacted has any interesting specimens at hand they would like to send me, give me a PM and i'll let you know where to send them. Hoping we can get a nice overview of the different species we have around and pair them up with their microscopic features we should get a better idea of what we've been finding. Also it would also be very feasable to include a basic TLC analysis of each collection to further the study. So anyone I have already contacted, a dried whole specimen would probably be best, i should be able to get that part of it done either next week or when im back home again in a couple of months. basically i'm aiming for this kinda thing using some weird subs as an example Share this post Link to post Share on other sites
mr me Posted June 6, 2010 Thanks for providing a microscopic view. What type of microscope are you using and what was the magnification used? Is there a way of staining specimens to provide more of a contrast? Share this post Link to post Share on other sites
ferret Posted June 7, 2010 ok that was probably a bit misleading if you didn't read the whole thing, those pics are P. subaeruginosa just used as an example as the kind of thing i'm aiming to produce. Haven't taken any pics yet of Gyms. Using a well worn second hand compound light microscope at 400x. Yep i'm going to try a few stains and see what works well, probably 10% ammonia and Lugol's, Melzter's and KOH would be ideal if i can get my hands on some. Share this post Link to post Share on other sites
ferret Posted June 8, 2010 so i've ordered some KOH for the microscopy, even though a trial with NaOH has looked promising. also 4-Dimethylaminobenzaldehyde for making Ehrlich's reagent for indole staining in the tlc analysis. if anyone is interested in either of the above drop me a pm, i'll have way too much for my needs... Share this post Link to post Share on other sites
Lord Mayonnaise Posted June 9, 2010 What a great undertaking. Will you be happy to receive samples from Victoria? Funny, those Subs are almost Gym-like. Share this post Link to post Share on other sites
ferret Posted June 9, 2010 yeah mate, will send you a pm Share this post Link to post Share on other sites
Zen Peddler Posted June 9, 2010 Good images sonny gym! Typical sub lageniforms and lechniform (sp?) c.cystidia. Or should I say typical of WA, Tas, Vic, NSW subs - SA subs look very different macro and micro. Share this post Link to post Share on other sites
sidewayschef Posted June 16, 2010 Hello Ferret, you get those samples yet? I have put up pics of the Gyms I found in the WA gyms thread Share this post Link to post Share on other sites
ferret Posted June 16, 2010 Zen - if you like those pics you should be nicely aroused by the series i took the other night of some of Lord Mayo's fresh specimens. interestingly i figured out i can take better pics with my crappy 3.2mp camera on the 1 megapixel setting, utilising the digital zoom. moving house at the moment and its taking ages but will put a few up over the next few days. sideways- i believe i got them today, a couple of young fruits of the burgundy and the yellow/orange ones if thats them? thanks heaps mate- been flat out so i've only had a quick glimpse. if you can can you also hang onto a more mature specimen of each just in case they haven't developed enough- will look at them over the next few days and will let ya know. Share this post Link to post Share on other sites
sidewayschef Posted June 17, 2010 to easy. have grinded the rest into a powder but will be having another pick this weekend Share this post Link to post Share on other sites
karode13 Posted June 22, 2010 Interesting thread ferret. I will be keeping a keen eye on your findings. Share this post Link to post Share on other sites
Alan Rockefeller Posted June 22, 2010 Interesting thread ferret. I will be keeping a keen eye on your findings. Yea me too. Check your shroomery PM's. I can post some microscopy of G. luteofolius from the west coast. And maybe some dna sequences. Share this post Link to post Share on other sites
Alan Rockefeller Posted July 6, 2010 What do you think of these? http://www.shroomery.org/forums/showflat.php?Cat=0&Number=12855026&page=0&vc=#12855026 Share this post Link to post Share on other sites
gerbil Posted July 6, 2010 (edited) missed this one really good work ferret. love the micrcope shots, i'm still running an old slide micro with mirrors for light source, yours looks kickarse. Those 'subs' are cool, looks like a cross between a cube Are they a melbourne find? Don't recall ever seeing stems like that on them, especially the consistency across specimens. Edited July 6, 2010 by gerbil Share this post Link to post Share on other sites
Mycot Posted July 7, 2010 What do you think of these? http://www.shroomery.org/forums/showflat.php?Cat=0&Number=12855026&page=0&vc=#12855026 I think they're a very interesting find. They don't appear to be G.purpuratus. They seem to be another new active gym species find for Australia. We seem to be having a nunber of these recently. Congrats. Welcome to the Corroboree Alan. It's good to see a person of your expertise here. Share this post Link to post Share on other sites
mutant Posted July 7, 2010 hey hello and welcome ... I still didn't send that strange southern europe sample to you not workman lazy bastard I am.... Share this post Link to post Share on other sites
Zen Peddler Posted July 17, 2010 come on fez - when you coming out too Sherbrooke? And I await your images eagerly. Share this post Link to post Share on other sites
ferret Posted July 26, 2010 unfortunately i didnt get time to do any work on these before i hit the road. will get back onto it in september when im back home. i found a couple of interesting red-purpleish specimens up cape york a couple of weeks ago too! Share this post Link to post Share on other sites
Mycot Posted July 26, 2010 (edited) Cape York. Wow thats pretty far north, an interesting find. Not too far from where the red-purpleish G.norfolkensis occurs. Edited July 26, 2010 by Mycot Share this post Link to post Share on other sites
ferret Posted July 26, 2010 (edited) Here they are, found in Iron Range. Sorry for the big size and crappy light.. also found some great ganoderma's up that way but they'll have to wait.,. Edited July 26, 2010 by ferret Share this post Link to post Share on other sites
Zen Peddler Posted April 16, 2011 Bump - Fezza - any updates? Share this post Link to post Share on other sites
ferret Posted May 9, 2011 (edited) well its pretty much been a year, and im almost ready to start. Hah hah. "Latrobe Gymnopilus" verrucose spores 7.4-7.8 x 5.4-5.7 (water mount, 0.98 microns per division) hyaline basidioles, basidia (congo red, 2.45 microns/div) hyaline cheilocystidia (18-)22-27 x 5.9-6.8 (congo red, 2.45 microns/div) Edited May 19, 2011 by ferret Share this post Link to post Share on other sites
ferret Posted May 12, 2011 (edited) "Mycot B" spores roughly 7.7-8.4 x 5.2-5.9 cheilocystidia (KOH, congo red) some pigmented basidioles?(KOH) basidia, mostly hyaline occasionally pigmentd(KOH, congo red) overall it looked kinda like this(KOH 100x) update with spores later and more stuff,... Edited May 15, 2011 by ferret Share this post Link to post Share on other sites
Zen Peddler Posted May 14, 2011 whats the background of Mycot B and are they penis shaped basidia/P.cystidia forms? Share this post Link to post Share on other sites
Mycot Posted May 14, 2011 (edited) Congrats on getting the microscopy underway. The images are beautiful, I'm really enjoying this thread. I really like the appearance of your latest gym find. Beautiful shade of red on those caps and the cutaway image showing the pinkish inner flesh an exellent touch. If I were to make a prediction I'd say active. Oh yeah, and I love the microscopy done on "Mycot B" as well. Interesting the clear difference in cystidia shapes between the two species. Excellent work my friend. Edited May 14, 2011 by Mycot Share this post Link to post Share on other sites
