ben Posted September 14, 2023 Share Posted September 14, 2023 (edited) Greetings all, i have recently noticed these black spots appearing on a few of my Cube cultures (on malt agar plates) i have attached photo's, doesn't seem to be any bacterial or pre-transfer contam. Seems to be a growth on the mycelium itself, any assistance would be much appreciated. IMG_2570.pdf IMG_2570.pdf Edited September 14, 2023 by ben Quote Link to comment Share on other sites More sharing options...
saguaro Posted September 14, 2023 Share Posted September 14, 2023 the .HEIC file isn't supported here, try uploading jpegs or gifs Quote Link to comment Share on other sites More sharing options...
saguaro Posted September 14, 2023 Share Posted September 14, 2023 Those are droplets of mycelial metabolites, not contamination Quote Link to comment Share on other sites More sharing options...
ben Posted September 16, 2023 Author Share Posted September 16, 2023 Thanks for the response, will look into see if there is any specific info out on black metabolites Quote Link to comment Share on other sites More sharing options...
saguaro Posted September 28, 2023 Share Posted September 28, 2023 these metabolites are there cos you're growing them for too long on the same plate. While you can get away with it, it's suboptimal to allow cultures to cover the agar surface. You should be subculturing and transferring mycelium from the leading edge. Quote Link to comment Share on other sites More sharing options...
ben Posted October 2, 2023 Author Share Posted October 2, 2023 cool thanks for the info, it has interestingly carried throught o the substrate (rye grain) as well, which is a lil weird i thought, i will, try some quicker transfers next round edge.pdf side.pdf top_of_block.pdf edge.pdf side.pdf top_of_block.pdf Quote Link to comment Share on other sites More sharing options...
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