ferret Posted August 11, 2014 Moghadam, A. R. L., Ardebili, Z. O., & Rezaie, L. (2014). Effect of indole butyric acid on micrografting of cactus. African Journal of Biotechnology, 11(24), 6484-6493. this was posted by hookahead in another thread but it may have been overlooked by many.. results look fucking sweet! lets experiment 3 Share this post Link to post Share on other sites
modern.shaman Posted August 11, 2014 (edited) Be aware that this paper shows the effect of IBA when used in vitro the results may not be the same ex vivo. Edited August 11, 2014 by modern.shaman Share this post Link to post Share on other sites
ferret Posted August 11, 2014 experimenters please note the optimum concentration was pretty low, 100 ppm, or 100mg/L. so if you have a 4g/L IBA solution, take 2.5ml of that and make up to 100ml with distilled water. you may want to PC it for 15 mins to avoid introducing pathogens, but im going to try it using fresh unopened bottles of IBA and water. 1 Share this post Link to post Share on other sites
modern.shaman Posted August 12, 2014 I'm gonna go out and say that 100 mg/L is actually a very high concentration considering that IBA is usually used at 1-2 mg/L for rooting. Share this post Link to post Share on other sites
AndyAmine. Posted August 12, 2014 I definitely find a temporary boost to the level of pupping in my cacti after a shot of IBA. 2 Share this post Link to post Share on other sites
ferret Posted August 12, 2014 andy how are you applying this shot of IBA? via watering can? I'm gonna go out and say that 100 mg/L is actually a very high concentration considering that IBA is usually used at 1-2 mg/L for rooting. the solution I can buy off the shelf must be diluted 40 times, i think most people reading will regard this as a low concentration. modern.shaman do you actually have anything to offer to this thread? if you are merely here to be a negatron could you at least be entertaining whilst you're at it? do you understand how they applied the IBA treatment? I dont think they were growing the plants in PGR containing medium, in which case 100mg/L would be very high even for rooting. rather I am going to wildly assume that the IBA was applied somehow topically to the graft union, or the stock plant watered with the solution.. Share this post Link to post Share on other sites
Dreamwalker. Posted August 12, 2014 just a guess...could it be mixed into lanolin? Share this post Link to post Share on other sites
AndyAmine. Posted August 12, 2014 Yeah via a regular watering of the roots. I should have clarified, I was using Nutriboost which has a few things in it but IBA is one of them. . Share this post Link to post Share on other sites
ferret Posted August 12, 2014 just a guess...could it be mixed into lanolin? yep it can be mixed into melted lanolin. i suppose you could smear a bit on top of the scion, and it would work it's way down through the tissues.. im putting a few seedlings on the chopping block tonight. Share this post Link to post Share on other sites
ferret Posted August 12, 2014 very fucking interesting, yes distantly related Family but should work? Artificial synthesis of interspecific chimeras between tuber mustard(Brassica juncea) and cabbage (Brassica oleracea) and cytologicalanalysis Interspecific chimeras between tuber mustardand red cabbage were obtained by in vitro graft-culturemethod. Before grafting, 6-day-old seedlings of tuber mustardand red cabbage were vertically half-cut and treatedwith different concentrations of 6-BA and NAA for 1 min,then, they were symmetrically fit together. As a result, sectorialchimeras were initially produced from the unitedshoot tips. The maximum frequency of chimeral bud formationreached 6.33% when the vertical sections of tubermustard and cabbage were treated with 2 mg/l 6-BA and1 mg/l NAA. When sectorial chimeras were propagated onMS medium containing 1 mg/l 6-BA, periclinal and mericlinalchimeras gradually developed. Chimeral shoots wererooted on half-strength MS medium containing 0.1 mg/lNAA. The rooted chimeras were acclimatized and transferredto the field for cytological and morphological analysis.The results showed that stomata density in the chimeraswas significantly higher than that of their parents, whilechloroplast size, starch grain size and number were intermediatebetween the two parents. The chimeras were furtheranalyzed by flow cytometry, and the results indicated thatthey contained both sets of parental chromosomes. Moreover,chimeral plants possessed valuable characters fromthe two parents. 1 Share this post Link to post Share on other sites
modern.shaman Posted August 12, 2014 After rereading the article again it seems like the graft is actually not done invitro. This would explain the reason for the relatively high concentration of IBA used. The reason I jumped to the conclusion that it was an in vitro application was due to the mention of it in the introduction and the term 'micrografting' is usually used in tissue culture publications. The application is most likely a foliar spray to runoff like GA3 applications are applied in greenhouses for uniform flowering throughout the nursery. I use to have an interest in creating a chimera however after further reading it's come to my attention that NO chimera is stable and the differentiating cells 'holding' the chimera will soon outgrow one another and will cause reverted growth of a single plant. What you would want is to create true hybrids by crossing flowers and using embryo rescue techniques to help underdeveloped or incomparable seeds to grow. Protoplast fusion would also be far superior technique for a true hybrid although not a easy task for home hobbyists. 3 Share this post Link to post Share on other sites