naja naja Posted May 22, 2006 Can I? I just don't have any agar material around at the mo' and wanna do a clone of the S.W. field mushrooms that have just popped up this week. cheers Naja Share this post Link to post Share on other sites
medicinedan Posted May 22, 2006 you can, but you will probably make things hard for yourself in the long run. Harder to spot contams. If It does contam, you cant isolate any clean myc ect.. Go the extra effort and do the agar I reckon Share this post Link to post Share on other sites
Hyphal Posted May 22, 2006 Can I?I just don't have any agar material around at the mo' and wanna do a clone of the S.W. field mushrooms that have just popped up this week. cheers Naja Yes you can man - make sure you wash the peice really really well first. So, are you saying, that our favourite fungus is popping up down south now?? Share this post Link to post Share on other sites
Eikel Posted May 22, 2006 (edited) Easy LC cloning.... Just use h202, since we're not dealing with spores. Requires: - Hydrogen Peroxide, 3% is the normal stuff, I use 50% but diluted before use. - Scalpel or cutting blade. - Containers for the LC and other stuff. 1. Make up your LC as per normal, then add 5ml of h202 per 150ml of water. 2. Pour a shallow dish of pure H202. 3. Cut your mushroom specimin down the stem and extract the healthy tissue inside with your scalpel or other tool. 4. Dip the piece you cut in the shallow dish of pure h202, just give it a quick coat. 5. Drop the now h202-treated piece of stem in the LC. Voila - LC cloning without contam issues! Agar + sealed petri-dishes are my preferred culture for long-term storage, but it involves a few more steps than an LC, plus LC's don't seem to contam as quickly/easily as agar (in my personal experience, which I admit is quite limited). - Just my 2c. Edited May 22, 2006 by Eikel Share this post Link to post Share on other sites
naja naja Posted May 22, 2006 Yes you can man - make sure you wash the peice really really well first.So, are you saying, that our favourite fungus is popping up down south now?? Nope, well actually I don't know, I havn't needed to check and I can't b fuct driving that far. I am talking about ACTUAL field shrooms, the type I could give out to family and friends. They have really thick stems, so getting clean clone material is no probs. I will use agar wen I get sum supplies, but just wanted to do a few clones now. I'm gona try and do them in kit form for a few friends presents. Cheers all naja Share this post Link to post Share on other sites
spiders Posted May 23, 2006 the problem with LCs is that they contaminate really reasily even with very high h202 levels. ANd its spread right through the medium without resistance like agar. Share this post Link to post Share on other sites
