Alchemica

Currently I'm finding I still have to not expect the kanna to do all the heavy lifting mood wise, it's really a tool that opens a potentiality for better states but without following through with behavioural activation/valued action you rapidly can sink back into the abyss. With a disability, I find the whole movement/dance/loosing your sh*t aspect nicely therapeutic to couple with the kanna as it's cognitively non-demanding and easily achievable behavioural activation at home that is not dependent on others. It's a sense of aliveness to life. Other areas I'm focusing on are: Connection. Improving some social engagements Independence. Gaining small steps of growth in self-care and independence doing very simple tasks myself Meaningful Pursuits Growth - for me, currently just trying to be content, happy "Dance is a pleasurable and captivating activity that involves motor, cognitive, visuospatial, social, and emotional engagement. Although practiced for thousands of years in rituals and as a leisure activity, the long-term effects of systematic dance on cognition, and brain structure and function are not well understood." Currently, there is increasing interest in dance as a therapeutic intervention for various clinical groups, ranging from developmental disorders, to neurological disorders such as schizophrenia (Martin et al., 2016) and mood disorder, neuromotor disorders such as Parkinson’s disease, to dementia prevention and management. Movement Medicine The efficacy of dance lies in its cognitive-affective-sensorimotor power to effect change’ and includes all aspects of the body-heartmind-spirit continuum. This continuum includes, transcends and bridges movement between the self, humanity, the world and divinity While for some it seem effortless, for some people it actually takes as much (or even more) courage and effort to choose an attitude of happiness, pleasure and gratitude as it does to let go of difficult emotions. It often requires an active choice, which needs to be renewed and renewed, instead of unconsciously and habitually emphasising struggle, suffering and hardship. It offers qualities such as: - being wholly present in the moment - an absence of thoughts - a sense of expansion or dissolving into the cosmos - distortion of time and space; experiences of non-duality - and a lost sense of self or ego If one can keep dancing through fear, the unknown, anxiety, avoidance and resistance, and eventually come out ‘on the other side’. Life seemingly becomes easier, and therefore they are willing to ‘invest’ in that reward. ‘Surviving’ these challenges often eventually results in increased self-confidence through knowing from experience that one is able to handle more than one initially thought. This is comparable to a ‘spiritual emergence’. Through the embodied experience of this cycle of descent, crisis and emergence, spirituality becomes a lived and living experience, rather than an abstract, external, metaphysical and transcendent concept Both the ‘feel-good feelings’ and the reward of emerging from a ‘dark night of the soul’ seem to stimulate happiness and well-being in general. One can consciously make an effort to increase and spread this zest for life. The psychological "holdings" in the body as the result of traumas, poor posture, or stress can be released, lulling the person into a "hookup" [peaceful, meditative state]:- given the choice between old painful "holdings" and new, freer movements, the unconscious will automatically choose the latter By moving the body, restrictive patterns can be changed, promoting relaxation and mental clarity. Also exploring therapeutic Drumming: Normally I'm an extreme stereotypic stimmer (flapping), which is one thing that kind of has no 'end result of health' associated with it, you can get worked up/modulate arousal levels but it doesn't have any personal sense of peace, connection, healing etc associated with it. The next level lately has been to embrace super unco 'drumming', which I've tried to get into a long time ago but I couldn't even manage to sense rhythm back then where my head was. Lately, I've been trying it again, learning to crank some techno and kill the rhythm fast as I can (starting just simply, tapping a table). I can attest, it does something quite nice to your mental-emotional-spiritual state, particularly at high bpm. Found it therapeutic enough that I want to ingrain it as a new habit over flapping, so whipped up a $0 drum from an old indoor planter and made it more interesting (second pic). "Drumming produces an altered state of consciousness and an experience of a rush of energy from the vibrations, with physical stimulation producing emotional release" My $0 drum It's able to calm one down and help a person deal with stress in their lives. “Drumming helps them to experience a kind of peacefulness and provides a spiritual learning context [potential to access a “higher power” and reestablish connections with their “natural selves.”]. Learning to 'drum' leads to positive changes in brain function and behaviour among those with mental illness [1] autistic people [2] and in addiction [3]. Improvements in: - hedonia (natural pleasurable experiences) - agency - accomplishment - engagement (focus and flow) - defining the self/self-awareness - produces the sense of connectedness - improved synchronicity between brain regions responsible for inhibitory control, which prevents impulsivity. - improvements to social skills and social well-being - reduces hyperactivity and attentional difficulties - enhances hypnotic susceptibility, increases relaxation, and induces shamanic experiences - release of emotional trauma - addresses self-centeredness, isolation, and alienation - enhanced sensorimotor coordination and integration [1] https://doi.org/10.1080%2F17482631.2018.1484219 [2] https://neurosciencenews.com/drumming-asd-attention-20802/ [3] https://ajph.aphapublications.org/doi/full/10.2105/AJPH.93.4.647 "

True but mood was too low to not quickly implement something as an option for mood enhancement. I wouldn't be surprised if the removal of the SSRI allowed a fuller range of feelings to emerge - removing the SSRI-induced emotional blunting and reducing induced akathisia - but during the week of washout, all emotions were extremely negative, it wasn't until the kanna was implemented that gradually positive emotions started to build. There were a few days of extreme emotional lability on the transition and after a couple of weeks the moods began to not be so volatile. I described a more longer-term leveling out of the effects of the kanna where "rather than the more acute robust uplift and transformative effects initially, almost a sort of tolerance to the effect but with improved baseline mental states" was noted "

I've been exploring mainly plain (?fermented) powdered raw herb (source Koda, sometimes Medicine Garden), at doses from a 1/4 tsp up and using it as added PRN as needed. Some of the other extracts on ebay etc seem potentially questionable I used a cold-turkey 5.5 half-life washout of the maximal dose SSRI and straight onto the kanna, just using plain herb. Naturally, the washout was really unpleasant but was only ~week to rough out which is easy enough when mood has been dysphorically shitty long-term. The first few days on the kanna were not overly remarkable but slowly an organic contentedness started to build which was solid basis for broadening and building other states from. Social things were still very challenging, probably harder during the transition which is understandable when you're in a state of change. Some of the case-study literature (as per article in original post's link) suggests therapeutic benefits in MDD, anxiety, personality disorders etc may be seen in the 10-14 day period Still good days and bad days but at least there are good days to build from

PM'd

Anyone used the stems/roots of this common ornamental and have experience with it? Exploring a common Apocynaceae - Catharanthus roseus The cytotoxic vinca alkaloid constituents are only found in the aerial parts of Catharanthus roseus (Figure 1), the roots are used in several countries as decocts or hot water extracts for the treatment of a number of conditions. The dried root is an industrial source of ajmalicine (raubasine), which increases the blood flow in the brain and peripheral parts of the body. Preparations of it are used to treat the psychological and behavioural problems of senility, sensory problems (dizziness, tinnitus), cerebrovascular accidents, cranial traumas and their neurological sequelae. In general, toxicity showed that both extracts and isolated compounds are safe to a certain limit, beyond that they cause adverse effects [1]. That said, the yield of vinblastine and vincristine from C. roseus aerial parts is is low, whereas the precursors, catharanthine and vindoline, are present in higher concentrations [2]. In the stem, there was in micrograms per milligram fresh weight (FW): catharanthine, 0.506 ± 0.044; ajmalicine, 0.071 ± 0.022; serpentine, 0.397 ± 0.031; tabersonine, 0.017 ± 0.003; and vindoline, 0.0026 ± 0.0002 (0.5mg/g fresh weight catharanthine in stem) Figure 1. The Catharanthus roseus plants used in this paper. The condensation of catharanthine and vindoline is an absolute requirement for the formation of vinblastine and, later, vincristine. As vindoline is required to synthesise the cytotoxic constituents but does not exist in the roots of C. roseus, but only in the green parts of the plant, no vincristine nor vinblastine can be found in the roots of this species In the plant, vindoline is a major constituent (up to 0.5%).Major alkaloids in the roots include ajmalicine, catharanthine, and serpentine. Another source mentions ajmalicine and serpentine are essentially present in the roots, whereas catharanthine and vindoline accumulate in aerial parts. The aerial parts contain 0.2-1% alkaloids [3] Roots to be used in pharmacy must contain at least 0.4% ajmalicine and serpentine Catharanthine (Figure 2) from Catharanthus roseus has been proposed to be a pharmacological treatment for addiction without the adverse side effects associated with ibogaine. It "slows DA reuptake and increases extracellular DA in the nucleus accumbens through partial inhibition of DATs" [4,5] and potentiates GABAARs [6] The root alkaloids from C. roseus root seem to be potent AChE inhibitors and catharanthine exhibited nicotinic receptor antagonism [7]. It has antidepressant activity via SERT inhibition and modulating NE [8] Figure 2. Catharanthine Ajmalicine has antihypertensive effects [9] Experimental: Root material was macerated in basified isopropanol (aq. ammonia) and concentrated to a small sample. TLC (silica, 0.2mm, glass backed, I2 visualisation) gave poor separation of the constituents with a mixed solvent of acetone:white spirits:1:1. White spirits gave better results with a major constituent Rf = 0.42 and some lesser Rf bands. [1] https://doi.org/10.1016/j.jep.2021.114647 [2] https://aiche.onlinelibrary.wiley.com/doi/epdf/10.1002/btpr.557 [3] Catharanthus roseus (PROSEA) - PlantUse English (plantnet-project.org) [4] https://scholarsarchive.byu.edu/studentpub_uht/242 [5] https://scholarsarchive.byu.edu/etd/9656 [6] https://doi.org/10.1016/j.bcp.2022.114993 [7] https://doi.org/10.1016/j.phymed.2009.10.008 [8] https://doi.org/10.1016/j.ejphar.2022.175454 [9] https://en.wikipedia.org/wiki/Ajmalicine

Thanks for picking that up, corrected. Just made it the phytochemistry but could add more when I'm not so zonked. Hope you're doing well.

Tried to do a simple Phytochemistry of Aptenia write up with TLCs Aptenia_TLC.pdf

Edit: GONE. If anyone doesn't trust their germination skills, can also offer a couple of free pots of seedlings - PM me. No WA/Tas.

Can offer free cuttings of Lampranthus spectabilis (Red) if interested. I can't properly explore as my serotonin transporter is heavily occupied but as it's a rampant grower and a different colour for the garden, it might be a useful addition. Have dried research material if needed too. Let me know in this thread if interested and I'll get back to you once the festive craziness has quietened down While it is claimed "...it would be almost impossible to achieve any pharmacological response from genera other than Sceletium" [1], recently Lampranthus species have been specifically marketed as "Chinese Kanna", alongside being used as an adulterant, one source stating Lampranthus spectabilis generally contains about 1–1.5% total alkaloids [2]. A high concentration of phenolics has been noted in Lampranthus [3], along with other phytoconstituents [4] "Of the five Lampranthus species tested, only L. aureus and L. spectabilis yielded mesembrenol, while all the other Lampranthus species investigated appeared to contain mesembrenone, but all at very low levels." Lampranthus aureus appears to contain other indolic alkaloids Mesembrine: SERT inhibition [other claims of 5-HT releasing activity], PDE-4 inhibition, Anti-inflammatory, Cytoprotective, Upregulates VMAT-2, Mild inhibition of AChE, Mild MAO inhibition, limited reuptake of NE and DA at high concentrations Mesembrenone/mesembrenol: SERT inhibition, PDE-4 inhibition [1] https://doi.org/10.1076/phbi.36.3.173.6350. [2] https://botany.bio/product/chinese-kanna-1-25-powder/ [3] https://doi.org/10.15835/nbha47411617 [4] https://doi.org/10.1016/j.sajb.2018.07.014

Edit: Gone. Germinated some Lampranthus aureus from seed, have a spare seedling if anyone wants to grow it. PM me. From [1]:

Thanks for the input, feedback and for reading Just random bits of non-Aizoaceae TLC info whilst experimenting Tabernaemontana undulata (root bark) and T. orientalis (stem) Samples were macerated in basified isopropanol (aq. ammonia), decanted and concentrated. TLC (silica, glass backed, 0.2mm, I2 visualisation) with acetone elution gave significant trailing and poor separation of the constituents for both. Elution with acetone:white spirits 1:1 gave some separations of compounds from T. undulata (Rf = 0.13, 0.35, 0.56, 0.89) but poor separation of constituents in the T. orientalis.(one potential constituent Rf = 0.75). White spirits elution alone gave a band of constituents ~ Rf = 0.24 for both Heimia Contains biphenylquinolizidine lactone alkaloids [1]. Plant material (leaf/stem) was soaked in basified isopropanol (aq. ammonia) and concentrated. Initial elution with acetone gave poor results but TLC (silica, 0.2mm, acetone:white spirits 1:1) gave distinct separation of compounds Rf = 0.43, 0.48, 0.55, 0.63, 0.95 Two (Rf = 0.43 and 0.95) were green-yellow/ yellow and fluorescent, the others plain visible pigments (green, blue-grey) [1] https://www.shaman-australis.com/~auxin/heimia.html Lobelias TLC with these posed issues in finding an adequate solvent system. L. cardinalis: Lobinaline as the major alkaloidal constituent (below) L. tupa: lobelanidine, lobeline and pentylsedinine etc (below) Dried aerial plant material was allowed to sit overnight in basified isopropanol (aq. ammonia), decanted and concentrated. TLC (silica, glass backed 0.2mm, acetone elution, I2 visualisation) gave a distinct compound Rf = 0.65 for the L. cardinalis but did not give any notable visible constituents for the L. tupa. TLC (silica, glass backed 0.2mm, acetone:white spirits 1:1, I2 visualisation) gave a distinct compound Rf = 0.40 for L. cardinalis and hints of a potential compound Rf = 0.31 for the L. tupa. Ashwagandha leaf withanolides Leaf withanolide constituents mainly include withaferin A and withanone Crushed dried leaf was macerated in isopropanol and the sample concentrated. TLC (silica, glass backed, 0.2 mm, I2 visualisation) gave poor results eluted with acetone but with mixed solvent (acetone:white spirits 1:1) gave at least four constituents Rf = 0.23, 0.33, 0.48 and 0.55 Exploring a relatively uncharacterised Leguminosae - Senegalia rugata Senegalia rugata syn. A. concinna - Sompoi or Shikakai - seen in Fig. 1 and 2. belongs to the Leguminosae family and is a medicinal plant widely grown in Southern Asia including India, Myanmar and Thailand. It is commonly known as shikakai and soap pod as it is used for cleansing of hair as a natural shampoo. The phytochemistry has been explored only to a limited extent [1,2]. There is also suggestion of internal use and the calyctomine (Fig. 3) has been used in the supplement industry [3]. Initial phytochemical study Figure 3. calyctomine Alkaloids, flavonoids, saponins, and phenolics were found in all extracts while phytosterols was found only in the ethanol extract. It yields lupeol, spinasterol, acacic acid, lactone, and the natural sugars glucose, arabinose and rhamnose. It also contains hexacosanol, spinasterone, oxalic acid, tartaric acid, citric acid, succinic acid, ascorbic acid, and the alkaloids calyctomine, a tetrahydroisoquinoline, and nicotine. Various other compounds have been isolated and characterised from the pods of this plant such as epigallocatechin, acacic acid lactone, machaerinic acid and its lactone, sapogenin-B, acacidiol, acacigenin-B, and kinmoonosides Experimental: Plant material was macerated in basified isopropanol (NaOH) and concentrated. Initial TLC (Silica, glass backed, 0.2mm, acetone elution, I2 visualisation) gave two main constituents, Rf = 0.14 and 0.59 but there was significant trailing of the compounds. Elution with mixed solvent (acetone:white spirits 1:1) gave a major constituent Rf = 0.65. [1] Screening of Secondary Metabolites and Antibacterial Activity of Acacia concinna http://dx.doi.org/10.3923/jm.2010.974.979 [2] Isolation and Evaluation of Anti-inflammatory activity of Epigallocatechin from Senegalia rugata along with PUFAs https://rjptonline.org/AbstractView.aspx?PID=2021-14-11-23 [3] Calyctomine HCL? https://anabolicminds.com/community/threads/calyctomine-hcl.282282/

Have some relatively uncharacterised plants and like to have a go at 'Citizen TLC' and give some plants the tender loving care they deserve on a different level? Can spare a couple of free packs x 10 of Silica plates (100mm x 25mm, 0.2-0.25mm on glass) and some micropipette tips for a couple of people You'd need the rest (jar, suitable solvent, visualisation eg iodine tincture etc) Let me know here if such a project is of interest to you. Please only express interest if it's genuine and you're willing to put time and effort into it and you'd like to, where possible, share your results.

After some peer scrutiny on the initial attempt and revisions, here is a rough draft. mesemb paper V2.pdf

Citizen Plant Science - Exploring the Aizoaceae Despite a long history of traditional use as medicines, the Aizoaceae remain an under-studied family of plants [1]. They often contain phytocompounds such as alkaloids, flavonoids, steroids, and their related intermediary compounds, one of the commonly found alkaloids is the mesembrine-type alkaloids [2,3]. These plants are well known in traditional medicine systems for antidepressant, anxiolytic, antimicrobial, anti-inflammatory, and antioxidant effects. Sceletium tortuosum (Figure 1.) contains mesembrine and derivatives (Figure 2.), an alkaloid not typically found in the emarcidum-type Sceletium spp. Other plants such as Delosperma pruinosum, and Delosperma pottsii [2] appeared to have some level of mesembrine but while Mesembryanthemum cordifolium syn. Aptenia cordifolia was initially thought to contain some levels of mesembrine, it seems instead to contain derivatives such as mesembrane sceletenone derivatives [4] which have in initial studies shown antidepressant activity superior to imipramine [5]. Figure 1. S. tortuosum Figure 2. Mesembrine and derivatives. Other Sceletium spp. have been traditionally used, S. emarcidum "was valued as highly as S. tortuosum in Southern Africa by different tribes and makes a very good Kougoed product". While apparently devoid of mesembrine, it seems to contain 4'-O-demethylated mesembrine-type alkaloids [6]. Mesembrine has been found in Amaryllidaceae such as Narcissus pallidulus and Narcissus triandrus. Other Mesembryanthemums such as M. crystallinum and M. nodiflorum have been suggested to contain some levels of mesembrine. More recently, Lampranthus spp. (Figure 3.) have been specifically marketed as Sceletium substitutes 'Chinese Kanna', or used as adulterants. These seem to contain mesembrenol and low levels of mesembrenone. Figure 3. Lampranthus and mesembrenol One early explorer called S. tortuosum ‘the greatest Clearer of the Spirits, and the noblest Restorative in the World’. Traditional use of Sceletium in South Africa often involves the collection and fermentation of the plant material, which has been studied and lead to greater levels of mesembrine and changes to levels of mesembrenone [7] and reduce oxalate levels. Mesembrine is a potent serotonin reuptake inhibitor (Ki = 1.4 nM) and to a lesser extent PDE4 inhibitor. Other notable mesembrine-type alkaloids include mesembrenone (also a potent serotonin reuptake inhibitor [Ki = 27 nM] and a more potent PDE4 inhibitor than mesembrine itself), and mesembranol. Another assay found that Sceletium tortuosum comprising of > 70% (w/w) stabilized mesembrine was a monoamine releasing agent [8]. The pharmacological properties of mesembrine and it's derivatives are quite diverse, showing anti-inflammatory, cytoprotective, VMAT-2 upregulation, mild inhibition of AChE, mild MAO inhibition, limited reuptake of NE and DA at high concentrations. Mesembrenol and mesembrenone seem to also share the SERT inhibitory and PDE4 inhibitory activitty. Clinically, the Sceletium spp. have shown antidepressant, anxiolytic and cognitive enhancement effects, case studies demonstrating increased attention, focus, and motivation while a patient with “a baseline mood of depression alternating with anxiety” felt “more focused, more engaged, and not so socially distant” after ten days [9]. Species such as Delosperma bosseranum (Figure 4) have been suggested by members of the ethnobotanical community to offer similar effects to Sceletium spp. but remain uncharacterised phytochemically and pharmacologically. Others such as Trichodiadema spp. (Figure 4), traditionally used as beer making roots known as 'Karee Moer' (T. barbartum syn. stellatum) as suggested to contain mesembrine but a detailed analysis is lacking (limited to positive alkaloid tests). Figure 4. The prototypical mesembrine containing plant, Sceletium and D. bosseranum and T. barbatum The purpose of this citizen science was to explore a diverse range of Aizoaceae for mesembrine-type alkaloids, including uncharacterised ones such as Delosperma bosseranum and Trichodiadema barbatum and screen diverse Mesembryanthemums for possible mesembrine-type alkaloids. [1] https://doi.org/10.1016/j.jep.2022.115988 [2] https://doi.org/10.1016/j.phytochem.2019.112061 [3] https://doi.org/10.1076%2Fphbi.36.3.173.6350 [4] https://def-sa.com/wp-content/uploads/2020/08/Gaffney_Candice_D_2006_0.pdf [5] https://doi.org/10.1080/14786419.2020.1788019 [6] https://doi.org/10.3389%2Ffnut.2022.819753 [7] https://doi.org/10.1016/j.sajb.2018.10.011 [8] https://patents.google.com/patent/US20160038551A1/en [9] https://www.researchgate.net/publication/328942189_Kabbo's_Kwain_The_Past_Present_and_Possible_Future_of_Kanna A range of Aizoaceae (Silica, I2 visualisation) gave diverse phytochemical profiles Trichodiadema stellatum (root) TS Trichodiadema bulbosum (leaf/stem) TB Sceletium tortuosum (root) ST Sceletium emarcidum (leaf/stem) SE Aptenia cordifolia AC ? Unknown Mesemb. Drosanthemum floribundum Dros. Mesembryanthemum (Red) [?Lampranthus] MR Mesembryanthemum (Gold) MG Mesembryanthemum (Yellow, squarish stems) MY Mesembryanthemum (White) MW D. bosseranum DB D. floribundum DF 1. acetone elution 2. acetone:white spirits:1:1 elution To TLC soon Tetragonia tetragonioides Mesembryanthemum crystallinum Lampranthus spectabilis (Red) D. echinatum

Anyone have clues on this one just from these pics? Edit: seems it's Mammillaria bocasana

3 more free baggies for interested people, last lot were snapped up quick.

I've been interested in this Lobelia as a potentially superior (?) alternative plant to L. inflata. 'The Penobscot people smoked the dried leaves as a substitute for tobacco. It may also have been chewed'. Lobinaline caused a significant, dose-dependent increase in dopamine release and preclinical and clinical data exist that support nAChR-based ligands as promising therapeutic agents for the treatment of depression, alcohol and drug dependence. The alkaloid has been proposed as a treatment for Parkinson's and psychostimulant abuse [1]. Lobinaline appears to be distinct from nicotine and lobeline in terms of its selectivity and functional effects at nAchRs and is a DAT inhibitor. Compared to other plant metabolites, such as nicotine and lobeline, lobinaline is relatively non-selective with respect to α4β2- and α7-nAchRs Lobinaline displays appropriate pharmacokinetics and low mammalian toxicity in mice relative to lobeline, the most widely studied Lobelia alkaloid. Haven't bioassayed myself but if anyone wants to grow it (say first 3 replies in this thread and PMs) let me know. Seed was collected from the plant pictured. No TAS/WA sorry. [1] https://pubmed.ncbi.nlm.nih.gov/27105955/

Both gone, should be more seed pods soon so I'll update soon

Bumping this topic up, I have two small plants available free if someone super keen missed out, let them root up a bit more before sending, they'll die back over cool frosty periods but warmer states or someone with a greenhouse might do well with them this size. Once again, sorry, no WA/Tas. While there is history of traditional use [1] and herbalists suggest it may be a nervine, contemporary use of the plant remains very limited. Lobinaline is devoid of the characteristic actions of lobeline and in mice, lobinaline is less toxic than lobeline (but did lower animals blood pressure) [2]. Toxicology suggests a large quantity ingested is toxic with symptoms reported as being "nausea, vomiting, diarrhea, salivation, exhaustion and weakness, dilation of pupils, convulsions, and coma" but some have used it in smaller quantities as a tea [3]. Lobinaline is an inhibitor of the dopamine transporter (DAT) in vitro and in vivo. It is more potent for inhibiting DAT (IC50 = 11.95 μM) vs lobeline (IC50 = 30-80 μM) [4]. In addition, lobinaline is a weak non-subtype selective partial agonist at nicotinic acetylcholine receptors (nAChRs) and a good free radical scavenger. it is likely that the DAT inhibitory actions are "atypical" in not having abuse liability. Early herbalists noted that a tincture induced the "disposition to sing" and preliminary bioassays by others have noted "...cardinalis is more like nicotine. Seems to give stimulant effects similar to mild nicotine. Seems to have mild aphrodisiac properties. Slight mood lift present it also seems to have anti-depressant properties" [5] The N-oxide has potentially superior dopaminergic activity [6], that said at 25mg/kg it lacked abuse potential in the conditioned place paradigm model and "at the dose we administered does not seem to have a significant effect in the mesolimbic dopaminergic pathways and may not facilitate a role in treating drug abuse" [7] [1] https://www.cargocultcafe.com/cardinal-flower/ [2] https://doi.org/10.1139/cjr38b-055 [3] https://eatwild.weebly.com/blog/cardinal-flower [4] https://doi.org/10.1016%2Fj.fitote.2016.04.013 [5] https://drugs-forum.com/threads/lobelia-cardinalis.213716/ [6] https://pubmed.ncbi.nlm.nih.gov/34648893/ [7] https://uknowledge.uky.edu/cgi/viewcontent.cgi?article=1013&context=medsci_etds

PM me an addy I can send to and you can have EGA journals 1 and 2 no cost, if I find #3 you're welcome to it

Have some rooted cuttings (3 x tubestocks) for people interested (No WA/TAS) Free including post. Just starting to root so I'll give them a bit longer before posting but express any interest here.

OK I'll reserve the above people for cuttings and get in touch soon. Thanks for the interest

I tend to hear of 6-benzylaminopurine being used for pupping which is quite a distinct compound (synthetic cytokinin with stimulatory effects on cell division) with a different mechanism to IBA That said, try it and see what happens. IBA has: - auxin-like effects such as root initiation, stem bending, and leaf epinasty - IBA-derived auxin has strong roles in various aspects of root development, including regulation of root apical meristem size, root hair elongation, lateral root development, and formation of adventitious roots. - IBA-derived auxin plays distinct roles in shoot development, with particular roles in cotyledon expansion and apical hook formation. Some studies indicate: - IBA application promotes elongation of stems in intact pea plants "IBA could act as an important source of auxin to boost stem elongation in intact plants" - In carrots it seems to cause longer plants "IBA significantly increased the overall plant length."

Anyone have personal experience with these plants? Curious as I can only find limited info on their use. Trichodiadema stellatum (syn. barbatum) This plant has been used as a yeast substitute for brewing beer and is reported to contain the psychoactive alkaloid mesembrine (Watt & Breyer-Brandwijk, 1962). Laidler (1928) also states that this plant known as kareemoer is “one of the beer making roots, a deliriant and intoxicant with an earlier stimulant action”. HARGREAVES 1998 notes that it is believed to contain an intoxicating alkaloid (“probably mesembrine”) Positive general alkaloid tests (ZWICKY) were had for Trichodiadema barbatum Mesembs reported to contain mesembrine alkaloids Trichodiadema barbatum (unconfirmed) Trichodiadema bulbosum (unconfirmed) Trichodiadema intonsum (unconfirmed) - FESTI & SAMORINI 1995 They have a nice fat root system that could be useful (similarly to D. bosseranum)

Just updating this - there seems to be confusion between A. cordifolia (Mesembryanthemum cordifolium) and A. lancifolia and there are common hybrids between the two. Aptenia cordifolia and A. lancifolia are very popular garden plants around the world, and are particularly useful as attractive ground covers on dry slopes as they rapidly form lush green carpets with decorative reddish purple flowers. A. cordifolia has cordate or heart-shaped leaves, while the leaves of A. lancifolia are lance-shaped, tapering gradually towards their bases. Leaf shape is used as a rather tenuous character in distinguishing A. cordifolia from its nearest relative, A. lancifolia.