omsource Posted April 27, 2011 I've just started using the take away PP containers for my petri dishes as they're re-usable as well as recyclable in the end. I couldn't stand the amount of waste i was making throwing out the PS dishes! I've been noticing MUCH slower growth on the dishes compared to the PS petris. Do the people that use them snap the lids on fully or leave them to vent? any help would be muchly appreciated Thanks a bunch OmSource Share this post Link to post Share on other sites
tripsis Posted April 27, 2011 (edited) Snap them on fully. I've found no issues using them as yet. How have you been using them? Edited April 27, 2011 by tripsis Share this post Link to post Share on other sites
omsource Posted April 27, 2011 Snapping them on fully, just seems like they don't colonise the plate half as quick. I've got some cultures you sent through that I did half on the PS and half on the take aways and theres a huge difference in time. Still the re-usability and the fact that I'm not mowing through the cling wrap is good! Share this post Link to post Share on other sites
tripsis Posted May 11, 2011 Interesting...never noticed a difference myself. I know someone in Melbourne who modifies his by cutting the edge of the container part so that the lid just sits on loosely. Share this post Link to post Share on other sites
themushroombloke Posted May 11, 2011 Interesting...never noticed a difference myself. I know someone in Melbourne who modifies his by cutting the edge of the container part so that the lid just sits on loosely. Another Idea: perhaps get a soldering iron burn a tiny hole then cover with micropore tape. This would offer better air exchange I assume. Share this post Link to post Share on other sites
satyr Posted May 25, 2011 So when using the PP container, do you guys sterilise them empty/separately and then pour the agar in still air box?! I thought I would safe the pouring-step and sterilised the agar filled containers...however after cooling down I now find relatively large amounts of condensed water inside on the container-walls, which is surely not ideal. Any hints welcome cheers satyr Share this post Link to post Share on other sites
tripsis Posted May 25, 2011 Sterilise first, then pour, or as you have found, you'll end up with excessive condensation in them. Share this post Link to post Share on other sites
omsource Posted May 28, 2011 Great point Tripsis! I've tried the "pour then sterilise" method and I've found that it creates way too much condensation, but also the cultures tend to contaminate on the outside lip, as the agar seeps out while sterilising. Just had that realisation this morning. How do you guys run a larger batch of empty PP take away containers? Do you seal them all individually and then PC them, or do the lids and the bottoms separately, or even leave the lids half on so that steam can penetrate into the containers? I quite liked your idea Tripsis where you did a large batch, poured them, sealed them and then had a stack for transfers whenever you needed them! Whats the process? OmSource Share this post Link to post Share on other sites
omsource Posted May 28, 2011 (edited) double post sorry OmSource Edited May 28, 2011 by omsource Share this post Link to post Share on other sites
omsource Posted May 28, 2011 (edited) ahh triple post, double sorry on the triple post OmSource Edited May 28, 2011 by omsource Share this post Link to post Share on other sites
tripsis Posted May 31, 2011 I sterilise them with the lids snapped on fully, then stack with in the PC and sterilise for half an hour (more or less depending on which PC I use as they have markedly different p.s.i. ratings). Steam still penetrates into the interior as they are not airtight (I don't bother with foil anymore). If there's room, I'll put the agar that I want to pour in with them, or do it separately. Then transfer the lot to a glovebox (or flowhood if you have one) and pour as you usually would. Share this post Link to post Share on other sites
mysubtleascention Posted June 6, 2011 A nice tek to try http://www.shroomery.org/forums/showflat.php/Number/13853861 Share this post Link to post Share on other sites
