Tripyamine

Probably because of the commercial value of some of the other products derived from reticuline/thebaine Just remember that the hoo-har is that there are essentially 3 magic yeast's -1 that can make (S)-reticuline from glucose -1 that can convert S to ®-reticuline -1 that finishes up the job and goes to thebaine or further So now it's just a matter of time for someone to combine all three.... But it's not that easy, there are complex regulation difficulties that would need to be overcome

Fun stuff http://www.theguardian.com/science/blog/2011/jun/21/scientists-make-lsd-from-microbes But if you get the biochem from the yeast -> opium paper (which it only kinda is - more about a cheap pathway to a general precursor) the scientists managed to beat the biosynth that poppies do in terms of steps/complexity

33

so much strong arm

A laminar flow hood blows, it don't suck..... its to stop contam not for your benefit You should have a good prefilter on the input..... like a MERV 11 or 13 cardboard from airepure

Hi guys I have seen a few of you have built HEPA filters some of which based around pleated hepa filters and not producing laminar flow and then some based on Separator HEPA filters So being the tightass that I am I thought I would see if anyone has found any cheap separator filters around Here is what I have so far (Im only going to use a 320x320) Aussiemushroomsupplies http://www.aussimushroomsupplies.com/index.php?route=product/product&path=27_40&product_id=123 ($110+$35 freight) H13 (32x32x15) Aliexpress http://www.aliexpress.com/item/HEPA-separator-air-filter/32269199585.html (about $89aud with freight EMS) H14 (32x32x22) Filterfit (look it up been mentioned before) H13 (32x32x15) Airpure (look it up) (rang and was quoted $220 for purecell separator) Still need to get a price from camfil and rainbow filters Im going to beef this post up with more goodness as I can be arsed

Le watch and le enjoy / I wish i could be arsed to figure out how to embed :/ https://www.youtube.com/watch?v=-vhViivTROs

Naturally: Firefly Sopranos V good and not mentioned: Leverage (This is one of the greatest shows ever, group of con men start conning rich people that have stolen from poor people, some old peter pan shit) Pushing up daisies

I dunno about the weird stuff, some plants have phytoestrogens so I guess it has basis as for the BAP yerh 8mg/L seems to be where its at but if you read a bit more they talk about 8mg/L BAP with 0.8mg/L IAA and the combination being responsible for the proliferation If you have the explants to spare you could run a matrix and do like 0.5mg 2mg 4mg and 8mg/L BAP and then 0.5mg/L IAA with 0.5mg 2mg 4mg and 8mg/L of BAP and see what works best Remember that the paper is for coffee, so its just a rough guideline (it may be wise to sacrifice 1 explant and use the optimal conditions of that paper, possibly half strength too)

Flowering is a pain in the ass, for most plants the signaling molecule is yet to be elucidated and I believe there are talks of it being an miRNA or a protein for example if you graft a flower to a plant that is not flowering you can induce flowering so there is signaling involved Icecube might chime in he has done some work on this Just forget you ever heard about suspension cultures they just don't yield for alkaloids (mg/l) but you could try upping the levels of tryptophan in your media as it is the precursor in the biosynth but again you kinda wanna be giving it to a big plant not a little tc tube, and then you might have troubles with absorption

Good thinking! escaped me about the sterility

This is a good paper - http://www.idosi.org/wasj/wasj2(2)/142-150.pdf In vitro Propagation and In vivo Acclimatization of Three Coffee Cultivars (Coffea arabica L.) From Yemen Naji Ebrahim, Rida Shibli, Ibrahim Makhadmeh, Mohamad Shatnawi and Abdallah Abu-Ein

Hi peeps Would love to see this little baby under the microscope if anybody has a print! Apparently they are down Vic ways so a wild print would be great! Got a few cool things to swap Cheers!

Gelrite is cheap as! Its clear and soft and you can see infection with ease It also is .3% not 3% so you need very little http://www.plantculture.com.au/GELRITE_50_Grams/p3017392_13618904.aspx Only $20 for 50grams enough for 150 odd liters of media

CO2 reg will fit it Type 30 thread on CO2 and N2O bottles Some med bottles have a pin index which will be hard to find a reg for

have you tried gelrite?

a.) you started from a small amount of stock and now have what you have, so cycling a few out to cover costs etc is just part of it, if you are comfortable with your technique and can keep repeating it then your rate of increase is still higher then your rate of loss c)like I said you started from a small number now your at 350, doubling 300 is a lot closer to a target goal of 1000 then what you started with d)until they are broken there is no standard for this, the plants will just start mutating and vital machinery will essentially break, your not really trying to stabilize anything so thats less of a worry e) there is a latent phase for the plant to re-establish itself f) if you get the hormones right you can use disk of leaf as the explant, you can even use use the petiole i.e allot smaller parts then the cuttings you're using infact a paper came out on this in 2013 which you can find for free here http://www.hindawi.com/journals/tswj/2013/209434/ < you will find a great deal of solid info in this paper, but you may struggle going from callus to a new plant as they have not covered that stage, if you wanted to put some decent resources into this, you could get a suspension culture of somatic cells and then possibly devide each one into an individual plant, but then from my basic search it seems the knowledge base is limited on the last few steps for this species, but you maby able to try coffee techniques

Too lazy and broke to order expensive 100mg lots from austratec so I was hoping someone on here might have some small amounts of some fun micropropagation chems Looking for under <10mg of the following NAA 2,4-D BAP TDZ WPM Just to start ill add to the list anything else I may need If noone can help I might buy small lots to pass around

I mite make a tutorial for you guys on some basic methods and techniques but go on libgen.org and look for "Plants from test tubes" The only hard parts are initially determining a compatible media and of course aseptic technique Now I just need some agent orange!

Boom Thanks so much his site http://www.plantculture.com.au/ Just hit almost everything I needed and its all so cheap!

Hey guys! havent been that active on the forums for a while, but I thought of all groups of people, you guys would probably appriciate this the most! I have been following the progress of this dirty pictures documenty since it was in production. To my knowledge there is still no sign of release on DVD or anything as they are still running screenings of it, I could be wrong please tell me if I am. So somehow last night I found a link to a viemo video of the whole documentry, now dont get me wrong I WANT TO BUY THIS FILM infact lets hope errowid gets a few signed coppies of the DVD and id donate my left leg for one But I have wanted to see this since I first heard about it 2 years ago so enjoy http://vimeo.com/29358948

http://www.seedman.com/flavor.htm The blueberry and the Mango are good, Im yet to try any of the other 50 new ones on that site. But yerh Ive gotten the best results with seedman stuff, tasty puff and everything else dint hold for long enough, like it was sick for a few days but then no more smell/taste

Biocurious

Im really interested in #4 can I buy it?

Sorry I didnt see that these had gone up if this is still around ill offer $75 a plant