Tripyamine

Keen! see you all there!

Also keen on sceletium please pm me!

@Darklight spirulina can get to 2-2.5g/L and other stuff like nannochloropsis will only get to 1g/L (more if thin-film reactors). Also thats bone dry. I personally prefer nanno both taste-wise and nutritional content-wise, its an omega3 (EPA) production powerhouse, you can expect to see 25% lipid and of that ~50% EPA Both have good amino acid profiles too but iirc nanno is more complete being a eukaryote and not a bacteria like spirulina 5g a day of nanno gets you an RDI of lots of stuff, but it's more an adjunct than a meal replacement. one day we will get there but I couldn't only eat nori (tastes like) soylent green is soon with nanno you can grow out (from 1/5) to a dense culture in 4-5days, and if you harvest 80% so you can regrow straight away then your collecting 15+g a 20L bag/5 days. ~200L standing bags are the way to go, if you only yielded 100g it would be enough for a week.

Hmmmmm I have a nice starter if anyone wants it le PM me I really like bags to grow in for a small scale, it's very popular in the industry too. If you get a bad infection/contamination you can burn your bags and reroll otherwise you can just keep reusing them. We use Polypropylene bag rolls and seal them ourselves to the size we need, and then sterilize with 70% ethanol and UV It's a good rule of thumb to always do a 1:5 inoculation with any algae (1:10 if you can't help it) I'll grow up a 5mll culture from a stock or a plate, scale that up to 25ml, then to 100ml, then 500ml, then 2.5lt and then start a bag culture with 10L and gradually increase the volume to 20L. Then its too a big 100-200L bag like the one pictured and then to ponds/pools all the way up to 400kL ^^^these are easy to make ill get some photos of our ghetto ones arthrospira (spirulina) likes to grow under basic conditions up to like a pH of 10 so lots of media reflect this as its useful for keeping lots of the contam/bugs out. Some good info on making the standard media here but you might struggle with some of the chems, also good culture info too. http://sagdb.uni-goettingen.de/culture_media/02 Spirulina Medium.pdf Another good media to use that you can purchase heaps of for cheap as is Cell-Hi JW (should be around $40 for a kg that will make close to 1000L), you will have to google around to find a supplier as we make our own and haven't had to buy for a while. At the end of the day if you scale up slowly and use appropriate media it's pretty easy to do, Spira harvests very easy compared to some other alga, but chitosan always makes it better! Sparge with lots of air and give em plenty of light (outdoors is better than LED but cheap eBay grow LEDs will work fine just gets expensive >100L)

Ypsilophora, ill take you up on the natives that sounds preemo for me! I'll put one established M. hirsuta plant on the table ;)

fixt!1!!!

Hi peeps Looking for some Catha edulis var narrow leaf Keen on seeds or plants Happy to trade or buy peas

HI there looking for a few grafted LW's or even other species of L, pref grafted Won't leave this up for long

Not much has really changed in the space, Illumina has a bigger machine and nanopore has released some new flow cells that are making Illumina and Pacbio quiver a bit methinks. I did a few algae genomes last year for $2k with nanopore kit, it's still a bit of a pain in Australia because there are no distributors so you need a specific Bicon permit and the shipping is killer. But long reads are amazing, just not accurate enough but great for resolving repeat sections in genomes, Illumina is still kinda unavoidable. SDN-1 is kinda funny, it has to be markerless to start with. From what I understand its no plasmid and no DNA. That means you need ribonucleoproteins ie the pure cas9 protein and a pure guide RNA that will randomly perform insertion or deletion and you don't even really know how many bases will get removed or inserted because it depends on what repair mechanisms are available in the organism you want to transform. This gets really tricky because you have only really one option, you have to fuck with a gene that will allow you to select a line that has your SDN-1 as well, this means it probably won't have it and just your selection in/del will be present. So there is still a lot of parallel replicates needed in order to get a line with what you actually want. You cant insert a gene, you can only remove its function or slightly modifies it. And yeah @Darklight the hardest thing about nanopore and Pacbio is the extraction, you need ultra-pure and unsheared DNA if you want to get long reads and the most out of your flowcell. Kinda funny but, there is a legend from ANU that has made a group on a website called protocols.io that has customized extractions people have perfected for heaps of different tissue. It took me the better part of 4 months to get my extractions clean and big/unsheared enough to run, I had to make up a whole custom protocol. Database access is still amazing, bioinformatics is mostly opensource still, between GitHub/NCBI/jgi/ebi basically everything is there, not everybody does it but you should put your raw data on the sequencing read archive (SRA) at the NCBI. So you can go on SRA make an account and reassemble peoples work with newer/better techniques/use it to beef your own sequencing up. Something I have been doing a lot of is metagenomics, which is heaps of fun. Take a sample with a complex mixture of organisms and sequence everything and see what's in the pool. Its a really cool diagnostic tool for anything from gut microbes to algae in a creek. I think Metagenomics is something lots of you guys might be interested in because its a way to look for pathogens (bacteria/fungi/virus/etc) with plants and is starting to get really approachable price-wise. I can do a 16/18s run on Illumina for ~$80 (but that's just prok/euk) and if you want to look at everything you just extract DNA and run it on a nanopore and blast as your sequencing, start to see everything in realtime which is cool for viruses and weird stuff.

Ok so PVA is a carbon source, plenty of things can grow off it as their primary carbon or even sole carbon source I guess the thing is what else is available and is there nitrogen and phosphate to facilitate things to grow/a bit of other stuff to satisfy any auxotrophy? I'm sure if you put some yeast extract or a rich nutrient source like molasses or malt then it would degrade much faster. Also if you oxygenate the solution then there is gonna be way more activity so movement definitely helps, ferments happen way slower without O2 unless anaerobic PLA - polylactic acid and PHA - polyhydroxy alkanoates Are both organic/biodegradable plastics eventually bugs will eat them, at around the same kinda rate as PVA, in fact in water treatment you can have massive columns of prills of these plastics and biofilms will form over them and deplete water of N and P, these are pretty common in marine aquariums. Here is a paper with some nice graphs, https://www.researchgate.net/publication/259284154_An_Efficient_Biotreatment_Process_for_Polyvinyl_Alcohol_Containing_Textile_Wastewater

Went a different route, feel free to delete

PM'd keen as a bean

oh lordy, moving around fastq files is a right pain thank the lord for nectar and aarnet filestore Beijing genomics institute just/is opening up in aus so sequencing might get a little cheaper here. At the moment its costing me about 3k to do the sequencing on say 12 different "things". I do transcriptomics so for me its 6 different treatments of a yeast and a replicate. Im a bit lucky and we have the illumina, iontorrent and pacbio rigs in the lab upstairs so its just the painstaking mRNA extraction and then library prep but, at that point it gets given to a tech and the magic happens. My research group (if its important) doesn't have to pay for sequencing, however, we have to pay for the kits and the flow cell for the machine so thats what the 3k is. Im lucky and can handle most of the bioinformatics myself so no need to pay for any of that which can get very exxy and ive seen it too many times in the past that they just flat out balls up the analysis and you get bad results, that should be fine but nobody ever double checks because they are 20gb and take a day to crunch at the least. It should be interesting to see how pacbio and nanopore drop the price, I cant wait to run something on a pacbio2 long reads is where its at for large plant genomes so much repeat that make it cooked to assemble.

Yerh I've isolated my own but I'm really looking for an old culture that's been going for a long time I know a few people have em in aus

Hi peeps Wanting to try brew some slick drinks up and am looking for a starter culture of ginger beer plant! I found cultures alive but no stock :/ Send me a PM maby I have something cool you may want! Willing to cover postage of course!