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The Corroboree


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Everything posted by Tripyamine

  1. Tripyamine

    Meet up: Brisbane

    Keen! see you all there!
  2. Tripyamine

    Kanna/Scelly Wanted

    Also keen on sceletium please pm me!
  3. @Darklight spirulina can get to 2-2.5g/L and other stuff like nannochloropsis will only get to 1g/L (more if thin-film reactors). Also thats bone dry. I personally prefer nanno both taste-wise and nutritional content-wise, its an omega3 (EPA) production powerhouse, you can expect to see 25% lipid and of that ~50% EPA Both have good amino acid profiles too but iirc nanno is more complete being a eukaryote and not a bacteria like spirulina 5g a day of nanno gets you an RDI of lots of stuff, but it's more an adjunct than a meal replacement. one day we will get there but I couldn't only eat nori (tastes like) soylent green is soon with nanno you can grow out (from 1/5) to a dense culture in 4-5days, and if you harvest 80% so you can regrow straight away then your collecting 15+g a 20L bag/5 days. ~200L standing bags are the way to go, if you only yielded 100g it would be enough for a week.
  4. Hmmmmm I have a nice starter if anyone wants it le PM me I really like bags to grow in for a small scale, it's very popular in the industry too. If you get a bad infection/contamination you can burn your bags and reroll otherwise you can just keep reusing them. We use Polypropylene bag rolls and seal them ourselves to the size we need, and then sterilize with 70% ethanol and UV It's a good rule of thumb to always do a 1:5 inoculation with any algae (1:10 if you can't help it) I'll grow up a 5mll culture from a stock or a plate, scale that up to 25ml, then to 100ml, then 500ml, then 2.5lt and then start a bag culture with 10L and gradually increase the volume to 20L. Then its too a big 100-200L bag like the one pictured and then to ponds/pools all the way up to 400kL ^^^these are easy to make ill get some photos of our ghetto ones arthrospira (spirulina) likes to grow under basic conditions up to like a pH of 10 so lots of media reflect this as its useful for keeping lots of the contam/bugs out. Some good info on making the standard media here but you might struggle with some of the chems, also good culture info too. http://sagdb.uni-goettingen.de/culture_media/02 Spirulina Medium.pdf Another good media to use that you can purchase heaps of for cheap as is Cell-Hi JW (should be around $40 for a kg that will make close to 1000L), you will have to google around to find a supplier as we make our own and haven't had to buy for a while. At the end of the day if you scale up slowly and use appropriate media it's pretty easy to do, Spira harvests very easy compared to some other alga, but chitosan always makes it better! Sparge with lots of air and give em plenty of light (outdoors is better than LED but cheap eBay grow LEDs will work fine just gets expensive >100L)
  5. Tripyamine

    Free Trade Thread 2019

    Ypsilophora, ill take you up on the natives that sounds preemo for me! I'll put one established M. hirsuta plant on the table ;)
  6. Tripyamine

    Anything to trade/Sell

  7. Tripyamine

    Narrow leaf Catha edulis

    Hi peeps Looking for some Catha edulis var narrow leaf Keen on seeds or plants Happy to trade or buy peas
  8. Tripyamine

    Looking for some grafted LW

    HI there looking for a few grafted LW's or even other species of L, pref grafted Won't leave this up for long
  9. Not much has really changed in the space, Illumina has a bigger machine and nanopore has released some new flow cells that are making Illumina and Pacbio quiver a bit methinks. I did a few algae genomes last year for $2k with nanopore kit, it's still a bit of a pain in Australia because there are no distributors so you need a specific Bicon permit and the shipping is killer. But long reads are amazing, just not accurate enough but great for resolving repeat sections in genomes, Illumina is still kinda unavoidable. SDN-1 is kinda funny, it has to be markerless to start with. From what I understand its no plasmid and no DNA. That means you need ribonucleoproteins ie the pure cas9 protein and a pure guide RNA that will randomly perform insertion or deletion and you don't even really know how many bases will get removed or inserted because it depends on what repair mechanisms are available in the organism you want to transform. This gets really tricky because you have only really one option, you have to fuck with a gene that will allow you to select a line that has your SDN-1 as well, this means it probably won't have it and just your selection in/del will be present. So there is still a lot of parallel replicates needed in order to get a line with what you actually want. You cant insert a gene, you can only remove its function or slightly modifies it. And yeah @Darklight the hardest thing about nanopore and Pacbio is the extraction, you need ultra-pure and unsheared DNA if you want to get long reads and the most out of your flowcell. Kinda funny but, there is a legend from ANU that has made a group on a website called protocols.io that has customized extractions people have perfected for heaps of different tissue. It took me the better part of 4 months to get my extractions clean and big/unsheared enough to run, I had to make up a whole custom protocol. Database access is still amazing, bioinformatics is mostly opensource still, between GitHub/NCBI/jgi/ebi basically everything is there, not everybody does it but you should put your raw data on the sequencing read archive (SRA) at the NCBI. So you can go on SRA make an account and reassemble peoples work with newer/better techniques/use it to beef your own sequencing up. Something I have been doing a lot of is metagenomics, which is heaps of fun. Take a sample with a complex mixture of organisms and sequence everything and see what's in the pool. Its a really cool diagnostic tool for anything from gut microbes to algae in a creek. I think Metagenomics is something lots of you guys might be interested in because its a way to look for pathogens (bacteria/fungi/virus/etc) with plants and is starting to get really approachable price-wise. I can do a 16/18s run on Illumina for ~$80 (but that's just prok/euk) and if you want to look at everything you just extract DNA and run it on a nanopore and blast as your sequencing, start to see everything in realtime which is cool for viruses and weird stuff.
  10. Ok so PVA is a carbon source, plenty of things can grow off it as their primary carbon or even sole carbon source I guess the thing is what else is available and is there nitrogen and phosphate to facilitate things to grow/a bit of other stuff to satisfy any auxotrophy? I'm sure if you put some yeast extract or a rich nutrient source like molasses or malt then it would degrade much faster. Also if you oxygenate the solution then there is gonna be way more activity so movement definitely helps, ferments happen way slower without O2 unless anaerobic PLA - polylactic acid and PHA - polyhydroxy alkanoates Are both organic/biodegradable plastics eventually bugs will eat them, at around the same kinda rate as PVA, in fact in water treatment you can have massive columns of prills of these plastics and biofilms will form over them and deplete water of N and P, these are pretty common in marine aquariums. Here is a paper with some nice graphs, https://www.researchgate.net/publication/259284154_An_Efficient_Biotreatment_Process_for_Polyvinyl_Alcohol_Containing_Textile_Wastewater
  11. Tripyamine

    All good

    Went a different route, feel free to delete
  12. Tripyamine

    Trich Eileen (90% sure on genetics) tips for sale.

    PM'd keen as a bean
  13. Tripyamine

    Ginger beer plant culture

    Hi peeps Wanting to try brew some slick drinks up and am looking for a starter culture of ginger beer plant! I found cultures alive but no stock :/ Send me a PM maby I have something cool you may want! Willing to cover postage of course!
  14. oh lordy, moving around fastq files is a right pain thank the lord for nectar and aarnet filestore Beijing genomics institute just/is opening up in aus so sequencing might get a little cheaper here. At the moment its costing me about 3k to do the sequencing on say 12 different "things". I do transcriptomics so for me its 6 different treatments of a yeast and a replicate. Im a bit lucky and we have the illumina, iontorrent and pacbio rigs in the lab upstairs so its just the painstaking mRNA extraction and then library prep but, at that point it gets given to a tech and the magic happens. My research group (if its important) doesn't have to pay for sequencing, however, we have to pay for the kits and the flow cell for the machine so thats what the 3k is. Im lucky and can handle most of the bioinformatics myself so no need to pay for any of that which can get very exxy and ive seen it too many times in the past that they just flat out balls up the analysis and you get bad results, that should be fine but nobody ever double checks because they are 20gb and take a day to crunch at the least. It should be interesting to see how pacbio and nanopore drop the price, I cant wait to run something on a pacbio2 long reads is where its at for large plant genomes so much repeat that make it cooked to assemble.
  15. Tripyamine

    Ginger beer plant culture

    Yerh I've isolated my own but I'm really looking for an old culture that's been going for a long time I know a few people have em in aus
  16. shotgun assembly is shit...... (ie putting a genome together usually gigabases together from 150base reads) As darklight said it costs a lot to build a decent scaffold and get the contigs (long linear DNA) to a small number (closer to the number of chromosomes the better.) Usually you start with a transcriptome (mRNA) to get a feel for expression and whats going on Sometimes mitochondrial or chloroplast genome sequencing is a better idea. You don't really need to have the whole genome, the paper mu! posted is not whole genome sequencing it is plastid or plastome sequencing which is just little dsDNA circles that provied sub functions in the cell like cpDNA (chloroplast) (which is a type of plastid) This avoids the need do assembly as the short reads can be stitched together from previous assemblies (scaffolding) and its not that huge so you can run a few plastomes in parallel (how the paper got so many done) on the one illumina chip which is what the "cost" of sequencing is, other then the couple of 12hr+ days you need to do for library prep if you had a few plants you wanted done (10+) and someone who was good at not fucking pipetting up or contaminating PCR reactions then you could do all of the plastomes for the cost of one "sequencing event" or you could make a snpchip that did it for specific lineages (cactus might be possible), which is based off nucleotides in the dna that change from plant to plant but doesn't really mean much in terms of function
  17. Found this today, nowhere else but the gold coast Hopefully you all get a laugh out of this, I know I still am and I found this at 10am http://www.goldcoastbulletin.com.au/news/veterans-outraged-at-red-nazi-flag-raised-at-a-burleigh-waters-home/story-fnj94j0t-1227322092600 Veterans outraged at red Nazi flag raised at a Burleigh Waters home JACK HOUGHTON AND ANDREW POTTS GOLD COAST BULLETIN APRIL 27, 2015 12:00AM Burleigh Waters couple Patrick and Doreen Hinks are disgusted at their neighbour who raised a Nazi flag over the Anzac Day weekend. A BURLEIGH WATERS man has infuriated his neighbours by flying a red Nazi flag in his backyard over the Anzac weekend. Elderly couple Patrick, 80, and Doreen Hinks, 79, woke early on Sunday morning to find the red Nazi flag fluttering in the wind by their backyard. Burleigh Waters couple Patrick and Doreen Hinks are disgusted at their neighbour who flew a Nazi flag on the Anzac Day weekend. The pair said they had been embroiled in a bitter dispute with their neighbour for more than three years and believe the Swastika was an act of retaliation. CRUISE SHIP PROPOSAL STILL AFLOAT “He has been trying to get us to stop flying the Australian flag in our backyard because he doesn’t like the noise it makes when he flutters,” Mrs Hinks said. “He has taken it too far this time because we can see the Nazi flag from our living room while we are watching the Anzac coverage. It is very offensive and he doesn’t know if some of our neighbours are Jewish and lost people in the holocaust in World War II.” Burleigh Waters man Patrick Hinks, 80, was forced to look at a Swastika from his living room during the Anzac Day weekend. When questioned by the Gold Coast Bulletin the man said he was within his rights to fly the flag. “It is not against the law and I’m not doing anything wrong,” he said. “I’ll take it down when I am good and ready.” Mr Hinks, who runs the Burleigh Waters Neighbourhood Watch, phoned triple zero when he saw the flag but was told nothing could be done. “Our grandson is in the army so this really bothers us,” he said. “We just want someone to come around and talk to the guy so we don’t have to see a swastika from our living room.” Queensland police declined to comment on the matter. A Nazi flag was pinned to a child’s trampoline over the Anzac Day weekend. Burleigh RSL sub-branch president Chris Keating said he was disgusted that the “sickening” flag had been flown on the Gold Coast so soon after Anzac Day. “It is just shameful to think of the atrocities committed by the Nazi regimen that this flag represents,” he said. “It is pretty sick and whoever is doing it cannot be right in the bloody head. There is no law against it which is why we are a democracy but we cannot let such a sick thing like this ruin Anzac Day.” Burleigh Waters couple Patrick and Doreen Hinks say they have been for year fighting with their neighbour over their Australian flag. The red, white and black flag of the Nazi party is legal in Australia and most other countries. Ownership or restrictions on the flying of the flag is banned in several European countries including Germany, France and Hungary. Burleigh councillor Greg Betts said he was disturbed by the flag’s use but warned the council had no power to order the flag’s removal. “As much as I would rather not see this sort of thing, there are no laws against bad taste,” he said. “Obviously this is a neighbourhood dispute but it is unusual that someone would go to such extreme lengths and I would rather they just sit down and talk about it. “Neither council or any other level of government has the power to remove it because the trade-off of freedom of speech is that people can display things which are distasteful to others.”
  18. Tripyamine

    LED grow lights...?

    Has anyone ever looked into Light emitting plasma? SOOOO SPENNO BUT
  19. Tripyamine

    LED grow lights...?

    And gas discharge has better peaks! Absolutely nothing wrong with Luxeon T mate (Or the arrays M and K) And seriously check out Bridgelux Vero COBs you will cream your pants, Drive them with LDD-1000's and you there
  20. Tripyamine

    LED grow lights...?

    The big thing with leds is their efficiency. for the most part a T5 fluro has about the same efficiency as chinese leds (epistar and the like) some are upwards of 100lu/watt - where epistar is around 85lu/watt (most t5's are 85lu/watt) If you want LEDs I think you are a fool not to get Phillips Luxeon M or L or K I really Like the K emitters some of these are 140lu/watt Im guessing most of you will use white from 3500k to 6500k for the most part COB's seem to be where it is at now and These little fuckers are the absolute ducks nuts http://www.bridgelux.com/products/vero-series 130lu/watt typical flux and they are in the best mounting package around! Kinda hard to get them in australia You might think I sound over the top here but really spending a little bit more at the start on good LED's will not only last years longer but will cost almost half to operate and put out the same amount of light, or really you should just buy a cheap dual T5 fixture on gumtree, I see 120cm fixtures all the time with ballasts for $5 and they always have hundreds from a job site or some shit Don't buy chinese LEDS they are shit and fluro tubes are better essential
  21. He's still waiting on the seed, be patient it's coming and worth it
  22. Ahhh kinda unrelated but good story none the less I was traveling in the philippines last year with my girlfriend and we were scuba diving around this little rock island a few km out from the mainland, we came back to the island for some lunch and to relax and we were eating some BBQ'd pork the guide had cooked for us while we were diving. Anyway my missus saw the guide feeding bits of pork to some of the little local puppys on the island so she immediately decided she was going to feed the dogs to... she held a bone up and one of the little puppys latched onto it and then jumped over a bit and bit her on the hand just the slightest bit but still drew a little blood. So at this point everyone on the island started freaking out about the possibility of one of the puppies carrying rabies, so one of the old pinoys minced up a few garlic cloves between his fingers and raked it into the bite then rubbed it off and rubbed more fresh garlic on, this went on for 20min and she was not happy about the stinging, he then taped a piece of garlic to her finger and sent us on the boat back to the mainland. In the proceeding 2 months she had about 15 needles just to make sure But when I looked on good old wiki, apparently it is the first port of call for a rabid bite
  23. I use a $20usd (shipped) blue plastic probe from Aliexpress, this is wired up to a simple pH circuit and fed into an ADC and to an Arduino monitored via the serial out This sits in a saltwater frag tank all day and the data gets logged to a xively feed; when the lights come on there is a nice shift from the photosynthesis kicking in, and it's all exactly the same as the colorimetric 7 to 8.9 test kit that I have which is highly regarded (sailfert) As for probe calibration, Im sure you're aware that 99% of calib is done by cleaning the probe/keeping it clean I use a 12 bit ADC and this generates a value between 0-4096 which is directly proportional to pH The main calibration factor used is drift, which is the measure of how different the probe's reading is to a "new" probe if that makes sense