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The Corroboree

Darklight

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Posts posted by Darklight


  1. Sounds good

     

    If you are starting from spores though, remember you can't add hydrogen peroxide to your media. Hydrogen peroxide kills spores

     


  2. 2 minutes ago, john said:

    Many thanks for the clear response. I just prepared some agar from a health food shop boiled with water - nothing else.

    I will try H2O2 as suggested in the mix next go. Mine have started to grow but are moving very slowly after 2 weeks. Maybe I 'm

    used to rapid growth in the tropics.

     

    K- so you have made what's called water agar

     

    It's not really nutritious at all and any growth you get could be resulting from residual nutrients in whatever it was you inoculated it with. That could be why growth is slow

     

    A general purpose agar is something like Malt Extract agar

     

    There are a million recipes for Malt Extract agar, often just varying the amounts. One that's worked for me for a variety of species is:

     

    20g/L light malt extract ( brewing supply shops sell it )

    100mg/L garden lime ( Bunnings )

    100mg/L potassium dihydrogen orthophosphate ( some people omit this )

    20g/L agar ( health food shops )

     

    A litre of agar is heaps! Like, about 40 standard petri dishes worth. So work out how much you actually need and change amounts accordingly

     

    If you want to add 3% hydrogen peroxide at 2-8ml/L, do that after you have cooked your agar and the container is at a temperature which can be held comfortably in your hand

     

    Malt Extract Agar is really nutritious. Lots of species like it- so do lots of contaminants.

     

    Not all species are happy with you adding hydrogen peroxide, but many will overcome their distaste for it and grow after a couple of weeks

     

    Good luck!

    • Like 2

  3. 13 hours ago, john said:

    I am starting out with shroom growing. I see a lot of recipes for
    agar. I have mycelium  growing in straight agar. Should I use a mix?

    When peroxide is used is that just for sterilizing or is it included in the agar mix. 

    sorry to be so ignorant.

    john S / Nimshie29

     

     

    Hi john

     

    Welcome to the wonderful, frustrating, fun world of growing mushrooms :)

     

    The agar question is a helluva lot to unpack. There are a bazillion recipes for 'agar' ( which is really only a generic word for whatever gelling agent is used to solidify the semisolid stuff in a petri dish, but the common use now includes whatever else is in the mix ). Confusing as fuck IMO

     

    Most people just use what's convenient for them that works, hence all the different agar types. Some types are specific to species, some are general, some used to deter competitors to get a cleaner culture etc

     

    Are you using only agar and water- your OP refers to straight agar- or is there other stuff in there, malt extract, dog food, vegemite, lime etc

     

    H2O2 ( hydrogen peroxide ) kills spore of bacteria and fungi without killing most mycelia- up to a point ( it can be overloaded, degrades if you keep the agar plates for too long, and shouldn't be added until your agar has cooled to the point where you can hold the container by hand )

     

    3% hydrogen peroxide is usually added 2-8ml/L when your agar has cooled and is ready for pouring. It can negate the requirement for pressure cooking your agar and is also handy if you don't have a sterile cabinet or still air box to work in. I've poured H2O2 agar in the open in my kitchen and had good success with it. I usually carry some plates of it with me if I'm taking samples from mushrooms I find on bushwalks

     

    You don't need H2O2 added to agar, but there are some situations which it really helps. Some species don't like it much and some take a while to establish on it

     

    Hope that clears things up. Good luck!

     

    • Like 1
    • Thanks 2

  4. On 03/11/2020 at 5:25 PM, prier said:

     My journey into plants basically started here so I figured this is a good place to announce I now run my own nursery.

     

     

    Hey Prier- that's awesome news, congratulations!

     

    Was really looking forward to seeing you at EGA this year, I've been coveting some of your lovely cacti offerings there for a long time and a catchup with you is always lovely

     

    Took me a minute to find your ebay store, but there you are:

     

    https://www.ebay.com.au/usr/whompingwillownursery

     

    You'll rock it! Coupla decades experience in cactii will serve you well :worship:

     

     

     

     

     


  5. On 08/08/2020 at 8:46 AM, sagiXsagi said:

    my father has started to seem hesitant to go to swim, he seems to be having a harder time to navigate and move his limpbs in water... 

     

    Sorry to hear about your dad mate, but it's beautiful you think of him so highly

     

    My dad's had Parkinsons for a long while, as have a few others I know, I've been following the research and their progress for a while. Unfortunately none of the people I know with Parkinsons are interested in support outside their direct relationship with their GPs ( who mostly don't know much about Parkinsons )

     

    But swimming's one of the best things your dad can do, if he's having trouble can you get him to wear a flotation device? I know there can be a bit of pride about wearing one in a public place... but swimming's so good for stages of PD. In Australia I've seen water exercise used as a recognised form of therapy for PD and it did work for my dad as a part of slowing his Parkinsons progression

     

     

    Quote

     

    I might seem like an idiot to be asking 

     

    No, you seem awesome and loving. I wondered about those compounds too back when Dad was diagnosed. I think you're right about potential deficits after use. Am not a physician and there are people here who are better qualified than I to explain the mechanisms

     

    There are some biological compounds I'd look at first before directly going for the pure stimulants

     

    Mucuna pruriens seed could be a good place to start if he's not already on meds ( buy it in, or only grow the hairless subtype )

     

    CBD is approved in Australia for treatment of some PD symptoms. Not sure about THC, but CBD is definitely on the list and I've heard ( and only heard- not seen yet ) good things of it for this. Not sure about legality/ accessibility where you live. Is most likely to be well tolerated alongside other medications

     

    Ibogaine root bark has been reported to reduce symptoms of PD too. There's a bunch of glowing reports on that if you google ( I do not trust glowing reports on Google much, but low dose ibo is awesome and I'd personally give it a go if I ever got a diagnosis) - but if you're recommending it to anyone else- particularly if they're already on other medication- I'd take real care to find a sympathetic primary health care professional and a good clean source of ibogaine root bark

     

    Was most interested to hear psilocybin/ mushrooms mentioned above, now I'll have to do some reading :)

     

    All the best for your and your family's journey.

    • Like 4

  6. 21 hours ago, lindsay said:

    recipe- 1 gram of chelated iron per 100 grams of dry grains.

    mixed into the cooked grain before sterilization.

     

    Whoa- lovely work, seriously impressive. Definitely warrants further investigation at other sites


  7. I've got an old Olympus ECEBi microscope and I love the damn thing. However it's a little dusty inside, and I'd like to do some spore identification with it, so I need crisp vision at high magnification

     

    A few mates have the same base model or a variant thereof. They were super common back in the day

     

    Yep, they're from the 70s or something, but they're well made. I love well made things :)

     

    Having trouble finding someone who will clean it- not just the outer, but the gubbins inside, which seems dusty

     

    Have spoken to a few professionals about it who have said it's a fair bet they're not worth looking at, and the average hourly rate ( including part thereof ) for a professional clean is about $200 an hour- that would also cover the evaluation, even if no work was done

     

    Bit lost with what to do about this. I'd also like professional advice on storing the damn thing so it doesn't suffer from fungus, which seems to  get into a lot of scopes in NNSW

     

    Anyone have actual experience with getting this done? Not just links- but actual experience?


  8. Yep, I wouldn't throw fungicide or glyphosate on it. Ick.

     

    Have a crack at throwing a fair whack of Aglime on it. Raising the soil pH might discourage that species and encourage a different one. Whether it's one you like or not is another matter

     

    If you have heaps of local used oyster mushroom straw  or other local spent mushroom spawn I'd throw that on top of the aglime.

     

    I haven't tried this, it's just something that's inline with basic mycological and biological processes. Inhibit the thing you don't want, encourage the thing you do

     

    And saguaro is right, there will be spores aplenty, and mushrooms will always come up when conditions are right for them and not their competitors

     

    Yellow stainers prolly occupy an important niche, don't wipe em out

     

    Let us know how you go

     

     

    • Like 2

  9. 17 hours ago, Tripyamine said:

    Hmmmmm I have a nice starter if anyone wants it le PM me 

     

    I really like bags to grow in for a small scale, it's very popular in the industry too. 

    If you get a bad infection/contamination you can burn your bags and reroll otherwise you can just keep reusing them.

    We use Polypropylene bag rolls and seal them ourselves to the size we need, and then sterilize with 70% ethanol and UV

     

    It's a good rule of thumb to always do a 1:5 inoculation with any algae (1:10 if you can't help it) 

     

    I'll grow up a 5mll culture from a stock or a plate, scale that up to 25ml, then to 100ml, then 500ml, then 2.5lt and then start a bag culture with 10L and gradually increase the volume to 20L.

    Then its too a big 100-200L bag like the one pictured and then to ponds/pools all the way up to 400kL

     

     

    arthrospira (spirulina) likes to grow under basic conditions up to like a pH of 10 so lots of media reflect this as its useful for keeping lots of the contam/bugs out.

    Some good info on making the standard media here but you might struggle with some of the chems, also good culture info too. 

    http://sagdb.uni-goettingen.de/culture_media/02 Spirulina Medium.pdf

     

    Another good media to use that you can purchase heaps of for cheap as is Cell-Hi JW (should be around $40 for a kg that will make close to 1000L), you will have to google around to find a supplier as we make our own and haven't had to buy for a while. 

     

    At the end of the day if you scale up slowly and use appropriate media it's pretty easy to do, Spira harvests very easy compared to some other alga, but chitosan always makes it better! 

    Sparge with lots of air and give em plenty of light (outdoors is better than LED but cheap eBay grow LEDs will work fine just gets expensive >100L)

     

     

     

     

    Wow. Just wow. Awesome, thank you so much! I knew nothing of these.

     

    What's the yield like for a domestic setup? Either per kg or how many single portion meals or meal additions you'd get per bag?

     

    This is now one of my one-day-I'll-get-to-it projects. Glad you got there first :D


  10. That. Was. Beautiful.

     

    Thanks to Ronny, Obtuse and Adele and the EGA crew for putting this together. Was very much worth the trip to Melbs.

     

    Panel was gorgeous, hell, the audience was gorgeous. A lovely mixed bunch of age and backgrounds, some were familiar with EGA's work, others knew more about the myco-side but quite a few knew of both.

     

    Audience had a pretty good grounding in all things mushroomy, which made for some excellent questions to the panel. The book table near the ticket point was often crowded with people who had at least one of the references if not more before they even got there. The venue was awesome

     

    The panel. I am such a myco-groupie. They all made themselves available for chats before the event and during intermission and a few stuck round afterwards and they were all fucken lovely people with a grace I can only envy. Question time was professional, comprehensive and respectful and thus also awesome

     

    If you get a chance, go to one of Dr Alison Pouliot's workshops or forays. She's got a really impressive schedule the next few months, quite a few covering specific species of interest ( how cool's that ) and apparently they book up full- fast. https://alisonpouliot.com/workshops-events/

     

    Sapphire and Fungi4Land: https://fungi4land.com/ about the most worthy book project of the decade. Love her work. I will buy all the t-shirts.

     

    Fungimap and iNaturalist. Best citizen science work on the entire planet ( I will find you that reference Cam, I promise ) Do they even need a link? Everyone knows em. Here, have the link anyhow https://www.inaturalist.org/projects/fungimap-australia

     

    Ah yeah, the film. Didn't get to see it. Sure it was fantastic tho, everyone seemed to like it :D

     

    Kudos to you all the lot of you. And thanks again

     

    Bonus points to me for being lucky enough to hang out with Obtuse heaps, and for working out how to use the bloody coffee machine thing at the flat

    • Like 2

  11. On 30/12/2019 at 4:14 PM, Calaverite said:

    Darklight how difficult is the tissue culture process?

     

    From seed? Easy. A great beginner's project, really worth a go.

     

    Sow a lot, some in individual containers, some with a few per container.

     

    Split the batch in half and put one of the halves in the fridge for 3-4 weeks then return it to your light rack.

     

    If you wanted to start from a live plant it could be more challenging. Start with seed in tissue culture and let us know how you go


  12. On 08/01/2020 at 9:21 PM, RonnySimulacrum said:

     

    The screening will be followed by a mycological panel featuring ecologist and environmental photographer Dr Alison Pouliot, mycologist Caine Barlow, ecologist Sapphire McMullan-Fisher and President of PRISM, Vice President of EGA, research fellow in Medicinal Chemistry at Monash University, and co-lead investigator of the upcoming Melbourne psilocybin trials at St Vincent's Hospital, Dr Martin Williams. The mycological panel will be facilitated by Cam Durnsford the coordinator of Fungimap.

     

     

    Ermahgord. I just saw this.

     

    I am very seriously thinking about heading down to Melbs for the night on the off chance I get to even be in the same room as the panel crew, all of whom are my idols


  13. 12 hours ago, Calaverite said:

    Based in Melbourne I am chasing some quality wasabi variety either division or seed.

     

     

    i sent some down to ReShroomed a few years ago ( mmm, maybe a decade now I think about it ). TC clones of seed grown. They grew down at Colac like corkers and the leaf size was 6x what I got in NNSW within weeks

     

    ReShroomed passed away a few years ago :( No chance of getting plants out of him now either...

     

    allrareherbs.com.au site says they're delivering Wasabia offsets in April

     

    But try the seed too. Even if you don't end up with a commercial-grade wasabi root, the leaves are great for winter salads and the stalks apparently are useful too

     

    Give it a go I reckon


  14. 36 minutes ago, Halcyon Daze said:

    Saved me and my neighbors so far, but some nearby homes have been lost. Even looting has been taking place in some areas, so it's a very sad situation for some. Pretty soon we'll all have to join them on the allied front, I can't see the situation getting any better in the coming decades.

     

     

    It is all so unbelievably sad I'm short of words. I hope you stay OK, and I hope it rains

     

    • Like 2

  15. 2 hours ago, niggles said:

    I took 9 large samples wrapped in paper towel collected with sterile mushroom knife stored in new takeaway containers ready and waiting for you. They come with gps coordinates time and date and photos of original fruiting bodies with a foot for scale. 

    Let me know what you want me to do with it :)

     

    Ooh er, thank you, I'll SMS you. I fell asleep and didn't do those plates yet, but they won't take long tomorrow

     

    I can't wait, this'll be awesome if we get it


  16. 2 hours ago, teamwhy said:

    laccocephalum hartmannii or a form of is my guess

     

     

    Nice :)

     

    2 hours ago, teamwhy said:

    here is a close up of the inside

     

    croppy.thumb.jpg.e2d3ca504c643f23d7d2846cc3496753.jpg

     

     

     

    Is that the inside of the pseudo-sclerotia or the fruit? I'm guessing the former. If so, it doesn't look very appetising, might be a good one to inoculate onto seedlings for re-veg work

     

    What are you up to tomorrow? I'll pour these plates out tonight. And try not to pour too many different agar types, we all know how that ends up

     

    If the inside of the fruit looks easier to culture and breaks up/ cuts well I'd love to grab a fresh fruit from either you or Niggles or both

     

    I'll bring some fresh H2O2 MEA plates for you both too. Best to have 'em fresh before the H2O2 goes off. Never know what you'll find in a hot forest :)


  17. 1 hour ago, teamwhy said:

    Sorry @Darklight truffle was probably a little bit misleading I should have said pseudo-sclerotia. I will post a picture later today. they are still pretty cool

     

    Is alright, I knew what you meant and I was into using the wrong term as well.

     

    Am most interested in getting a tissue sample onto fresh H2O2 plates ASAP. Cancelled all other appointments today, making plates now.

     

    Niggles is hunting samples as well. What are the chances of me getting some tissue samples off you late sarvie/ do you want me to bring over some fresh H2O2 plates?

     

    What tree species are the pseudo-sclerotia originating from? I might have to make up some woodchip media for it

     

    I'm so easily distracted....


  18. 10 minutes ago, teamwhy said:

    check out these guys that are popping up everywhere after the fire. 

    crop.thumb.jpg.da6183a89a13cde9c05f8ad66e7e4332.jpg

     

    You reckon they're the same as Niggles is finding? Check thread

    http://www.shaman-australis.com/forum/index.php?/topic/46677-found-growing-in-the-ash-from-still-burning-bushfires/

     

    10 minutes ago, teamwhy said:

     

    I will go see if I can dig up a truffle this week some time.  I so want to eat one they smell so good! 

    Probably some Laccocephalum spp.  but can find a picture of any that fit. 

     

     

    WHAT? THERE ARE TRUFFLES? YOU'RE FINDING TRUFFLES?

     

    @teamwhy Get tissue samples and dry em proper, at least 200mg. I'll add them to the collection of things that need sequencing ( special kudos to Obtuse, who explained at length why this should be done properly )

     

    Put some fresh tissue down on H2O2 MEA agar plates. If you don't have any I will make some of the bastards up. Niggles might have access to some too, but they will be a coupla months old

     

    *sigh* you prolly don't have time, but if we can grab some properly identified edible native truffles we will all be well stoked, and we can try to add some to any regen work.

     

    Anyone else got any favourite firies out there?

    • Like 3
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