smogs Posted November 21, 2003 are there antibacterials you can use to stop bacterial growth but not inhibit fungal growth? such as eucalyptus oil or something?? obviously u still have to be vigilent against other fungus but its 1 less thing to worrie about. Share this post Link to post Share on other sites
Rev Posted November 22, 2003 Antibiotics - old veterinary prescriptions are good (streptomycin, chloramphenicol and some others - avoid gentamycin as its quite toxic to humans). crush n dissolve then filter sterilise into agar just before pouring, or if heat stable add to agar before sterilisation with a big but... You really dont need them sure you could use them but if you were to rely on antibacterials then you are probably not using proper technique and perhaps not producing a product fit for consumption I dont use any antibiotics in general practice I do have some tubes for when i go out into the field but in the lab peroxide is enough Antibiotics are useful in cleaning up cultures with hidden bacteria on agar - Enoki is awful for this, or for cloning wild mushrooms but on agar bacteria is normally very easy to deal with - its moulds that give you the trouble and the reproductive cycle of these can be interrupted with peroxide (spores die) in Aerobic coditions many species will eat bacterial colonies if given time but you dont put them in the substrate Share this post Link to post Share on other sites