fungi and slugs

[fungi and slugs]

Yep I do agree, in a controlled environment, would be then real science.

 

A 25 ltr bucket with a lid, some wet rocks in the bottom, so they can hide.

and say 10 each, of each type of slug (I may have 3 types here) or 30 in total.

a tray with some young broccoli leaves and pea sprouts. so you knew you were

no starving them to death, and a tray for the bait. some observations say 2 x per day.

 then in 3 days do a head count of the living or dead.

 

I do know it works myself. it just was not, until the other night did i notice the contrast

in what i could see. I had seen snow peas were just busting through the ground that day.

so i took a look that night. The year before they took out half of them and had to

replant the gaps. this year they are up a inch and only one has been chewed at.

i have be at this place for 3 years and have never know a place so chronic for slugs.

the first year I squashed hundreds and getting close to a thousand.

the second year i use a iron based bait i got online and this worked, but was not cheep.

this year its costing me next to nothing. 

someone else needs to run a trial.

I am just one man who is happy with a victory.

if can help someone else then i may have two.

 

[Serious Slug problem - Solution needed!]

 

[fungi and slugs]

Had some trouble with slugs last year. so I purchased some organic

slug killer. when I read on the packet the active constituent was iron,

something went click. 

I had observed when I put my mushroom off cuts on the compost heap.

then look at night, the mushroom would be covered in slugs, "a real 

attractant". I was also aware that mushrooms accumulate heavy metals.

so I added some chelated iron to some grain and inoculated with some

oyster mushroom. this spawn works fresh and dried.

it takes about 3 days to kill the slug, by stopping it digestion.

 

5 nights back i took a quick walk around the garden with the torch

and easily could have counted 100+ slugs over a cm in size.

so I through out some dried spawn. and last night when i took a look

all I could find was some really small ones, recently hatched I guess

and the few larger ones I could find seemed dehydrated.

 

recipe- 1 gram of chelated iron per 100 grams of dry grains.

mixed into the cooked grain before sterilization.

[Cordyceps Militaris growing]

 search Azomite ebay, ozy seller $5.60 for 100g or 1kg $20 free postage.

good for compost worms as well.

[Cordyceps Militaris growing]

found this facebook group the other day.  you may already know of.

have not gone through much of it as yet, but this post may shed some light. https://www.facebook.com/groups/1449554748397163/permalink/3257908330895120/

there is a huge amount of info to filter through on this facebook group.

[Cordyceps Militaris growing]

Harvested some trays few days back.

This tray was started 30/5/20

Harvested 18/7/20 

7 weeks from start to finish.

Used 2 different size trays, and I noted that in one size

tray the cordyceps were large and fat. in the other they were

thinner but more of them. The influence was substrate thickness.

So I done some math, measuring the Base of the trays and looking

the amount of dry rice that went into them.

This is how it looks

16cm x 7cm =112cm square, 75ml rice = .67ml per cm square big fruit.

11cm x 18cm = 198 cm square, 100ml rice = .5ml per cm square small fruit.

My new bigger trays are 15x22cm and by chance they are .58ml per cm square.

right in the middle of these 2.

yet to do a harvest weigh in of each tray size. still sitting in the desiccant chamber, 

finishing off. should learn some thing from this.

[Cordyceps Militaris growing]

Part 3

the rice and broth go into trays, with foil over top

and cooked for 20 min at 15 psi in a pressure cooker.

 4 trays 26.5 x 19.5 fit in the presto cooker.

the plastic storage container are prepared adding 40ml of a Sodium

percarbonate solution. the the stuff used for home brew. shake

around inside the container, making contact with all the surfaces.

when ready to enoculate, in front of a flowhood crack the corner

open and pour the solution out into a jar or something.

then open the lid.

 

drop the tray in and peel the foil off.

with these big trays i use 15-20ml LC zig zagged across the surface

off the substrate.

I have melted 2 holes into the top with a metal sewer and covered 

over with micro pore tape. the containers are not a tight seal so the

holes may not have been necessary. 

 

after 3 days at temps 18c-20c in the dark they look like this.

at this stage put into fruiting.

fruiting temps should be kept below 20c. 

lighting are 5050 grow leds strips of a ratio 3 red 1 blue.

 

  

 

[Cordyceps Militaris growing]

same brand as i got and ozzy supply.

https://www.u-buy.com.au/catalog/product/view/id/12138166/s/himedia-rm007-100g-soya-peptone-100-g?gclid=EAIaIQobChMIvJyO2p_O6gIVCH8rCh0JKgIIEAkYASABEgJK2PD_BwE

[Cordyceps Militaris growing]

give me a day  i could send some through

at cost price plus postage to get you started.

 

[Cordyceps Militaris growing]

Amazon is were got peptone from. was the cheapest I could find.

got a 100gm forgot the price. its great in the LC, how necessary in the broth

i dont know. i must test.

bit mowed down at work for a few days at the moment.

may some days before i get back to the rest of this topic.

will log into amazon tonight and find the product again.

 

[Cordyceps Militaris growing]

part 2

 

my grows are on brown rice. there a many grains cordys grown on

quinoa, birds seed, soya bean or a mixture.

rice is cheep and easy to get. there a organic source that

I hope to track down. 

 

just like cooking rice to eat. you go 2 cups water to 1 cup of rice.

in this case broth.

Broth recipe 

2 cups water
7g dextrose
2.2g cornflower
5.5 g insant dry yeast
2g azomite
.5gm peptone

I aim for about 15mm of cooked substrate in bottom

of the tray.
 

[Cordyceps Militaris growing]

Part 1 

doing in parts--lost 2 hrs work last night -- done something stupid 

 

I have been interested in the health benefits of cordyceps for some time now.

the main benefit i was looking at is the increase in exercise performance.

I take a 1.5 gm tea 20 min before my workout. can easily say it gives me

up to a 10% increase in the intensity of my workout and it makes me feel good. 

so I am hooked. 

my goal at the moment, is to grow enough through the colder months to

last me and mum, until its cool enough to start again.

there is a heap of health benefits, just  google search cordyceps health.

 

Now to why I have started this topic. that is, to show how I have gone about

growing this great mushroom so far.

So I hope, with this bit of info I can help others get started.

I started off growing in jars. but with a 23 quart presto pressure cooker the

harvest volume was not cutting it with me. a lot air space in the cooker between

jars and having to inoculate each jar had me turn to aluminium baking trays.

inside a plastic storage container .

  

started with 2 small trays, then a medium and now I have a larger one that fits

perfectly into the cooker and into the storage container.

 

To start a grow, liquid culture is the way to go. with a 5 mm agar wedge

in 250ml it takes no longer that 6 days to fill the liquid. and I do mean fill it.

   

these jars are 4-5 days old. as you can see, peptone adds good vigour to the

culture. malt is ok without the peptone.

#1
250ml water
1gm light malt
#2
250ml water
1gm light malt
.5 gm peptone
#3
250ml water
1gm dextrose
#4
250ml water
1gm dextrose
.5 gm peptone

[Cordyceps Militaris liquid culture and slant]

All to happy to help where i can rottenjonny.

The log will start with the grow i am about to harvest.

and the steps that were done to get to that.

i have just inoculated a new grow and have another just starting 

to fruit. i take pictures all the time. so i hope i can put together

some helpful info together and learn somethings myself. 

 

[Cordyceps Militaris liquid culture and slant]

only 1 LC syringe left

[Cordyceps Militaris liquid culture and slant]

------------All gone-------------

i have 2x 30ml syringes $35 each and a slant $45

this includes postage.

this of my own isolate, first slanted 10/5/2020. The LC

and a new slant was taken from this. so is a fresh culture.

Note cultures from this fungi don't keep well in long term storage.

6 months max in the fridge. after this it will grow, but not fruit.

offer is open only till monday midday. after that, it will be a week

before i get another chance to post.

put your hand up here and then pm your addy.

i will be putting up grow log in the mycology section

on this site by monday. including recipes i use.

i am no expert, so be open to ideas and experiences.

 

 

[Grifola Frondosa]

 

 

[Edible Cultures for Sale Australia]

Nice collection ace1928. Can i entice you out of  hibernation.

i have selected a good number from your list. it should make it worthwhile

 

I have spare slants to trade of

Black Poplar (Agrocybe Aegerita) 

Morel (Morchella Angusticeps)

lions Mane (Hericium Coralloides)

i have a good number of others, but need to test grow these isolates

before i can offer out.

 

Here is my wish list.

i am after fresh plates, that have not grown to the outer edge.

a centimetre of growth is fine.  happy to pay fast post.

 

*Pearl Oyster

*Monster - Victorian Origin Wild Pearl Oyster

*Victorian Blue Oyster

*Sordid Blewit - Lepista sordida

*Maitake *Black Morel (morchella importuna) *Hon-Shimeji/Beech *Paddy Straw *Pink Oyster *Golden Oyster *White Ferula (pleurotus nebrodensis) *Milky Mushroom (calocybe indica)

[[Question] Identifying Gourmet/Medicinal Mushroom cultures]

The supermarket is a great way to get your hands on cultures cheaply.

i have done so a number of times with great success.

as Maximit says myc is generally white.

Here is a swiss brown mushroom from the supermarket, growing on peroxide agar. this is the second

transfer. The browning in this plate is the fungi's  metabolites that showed  up as the myc got towards the edge of the plate, but started pure white.

peroxide agar is the way to go.

great for wild mushroom as well.

Here is a good read on this peroxide tec. http://jontrot.free.fr/champignons/culture-eau-oxygenee-Vols1-2new.pdf

[Mushroom culture confusion]

 To the question is sequencing techniques the only way to determine a genetic difference.

My thoughts are if you put the 2 cultures in question, on agar and they were the same.

then they would grow together seamlessly. and if they were different there

would be a division of some sort.   just like a multi spore inoculation on agar, sectors can

be seen. 

[Seeking medicinal fungi - reishi, lion's mane, turkey tail, cordyceps]

Polyporus versicolor

http://www.aussimushroomsupplies.com/index.php?route=product/product&path=101_127&product_id=241

 

Ganoderma lucidum

https://www.miltonmushrooms.com.au/store/p11/Reishi_culture_(Ganoderma_lucidum).html 

 

 

i would love to get my hands on Cordyceps militaris as well.

 

[Lion's Mane Supplements]

you can get grain spawn here but is Hericium Coralloides not Hericium erinaceus.

Can any one tell me has Hericium Coralloides the same benfits as Hericium erinaceus.

http://www.aussimushroomsupplies.com/index.php?route=product/product&path=101_127&product_id=677

 

i grew Hericium erinaceus from a culture i got from someone here. i would love get my hands on it again. 

 

[Lion's Mane Supplements]

Host Defence (search ebay) is the company that stamets grow for or owns, and would be the most reputable.

they have a high price tag, but when worked out on a daily cost it less than cup of coffee.

I do not have experience in the usage but.

Reishi is my mushroom favourite. i have been taking Reishi for over 18 months now and have

not even sniffle this winter and have experienced many other benefits.

there are some good suppliers of mushrooms in India that sell caps or in powder forms.

 

just as a note when stated 13:1 or 10:1 extract, this is the amount of mushroom in one part of

product. eg 10:1 = 10gms of mushroom in 1gm of product 

 

[This section needs a "show wild finds" thread]

I love my walks in the bush.

Not only do I get to enjoy all the sights, smells and sounds.

I also get a great form of exercise. ducking and weaving through the scrub,

crawling over and under logs, jumping over creeks, up and down hills across

uneven, unstable and slippery terrain.....   ok point made.

 

over 3 months in a area of about 1km x .5km over 50 different mushrooms found.

here are some of my favourites.

   

   

    

    

    

 

 

 

 

[microscopy prints wanted]

 I am after a Cubensis print for microscopy. 

 don't have a lot to trade. just getting back into it.

These maybe of interest to someone. 

      

or i can add $20 to my $50 intended donation to this forum.

any old non-viable prints, of this au species will do. 

 

[2018 Winter shroom pilgrimage.]

go to this page there are 9 pdf's. https://www.fncv.org.au/fungi-in-australia/  i have on my tablet that i carry in my back pack. very handy. enjoy