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cornilius maximus

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Posts posted by cornilius maximus

  1. Posted

    Hello people,

    I am preparing a uni assignment on Native Australian/Oceania plants that display antidepressant properties.

    I'm not targeting any specific receptors etc. as such, I'm even interested in general well being properties.

    Anecdotal properties are also relevant for this project.

    So if anyone in the community can guide me with this it would be gratefully appreciated.

    peace

    cornillius maximus

  9. Posted

    You should only use the recommended dosage for any plant growth regulator i.e. GA3, IAA, BAP. 2-4-D

    For example; 2-4-D is a synthetic auxin that is used as a broad leaf herbicide in high concentrations, but when auxin is used in lower concentrations as produced by plants it is a root stimulant, i.e. striking powder for cuttings.

    Maybe try a concentration gradiant to see what works best?

    peace

    cornilius maximus

  12. Posted

    Hi All,

    I was quite intoxicated when I wrote my first post and in doing so forgot all about it. :blush:

    Darklight, thanks for your input.

    Ooh fun!

    Wasabi, Lotus or Corydalis. They've been done before I think so they'd be relatively simple protocol wise and you could just tweak the protocol to try for enhanced yield- much easier than starting from scratch

    Iboga if you're somewhere that can handle any legal restrictions ( if there are any- not sure ). It's been done in suspension before to not a lot of yield but you could also experiment with pre-feeding or elicitors or different culture regimes like continual harvest from immobilised cells. As a student you don't need to prove more than a solid experimental foundation and good documentation, you're not after commercial yields

    Lotus.

    Why are roots the preferred starting material for your culture? Why not embryonic callus? Roots are a bitch to sterilise unless you have them established aseptically already, and if you have aseptic seedlings you may as well start from all parts

    Why suspension culture? Why not hairy root culture? It's more reliable over multiple generations as a producer I think, and cell suspension lines need good solid storage and record keeping or they degenerate quickly

    I've a million ideas for this. Let us know what you pick and good luck

    I have only just began discussing this with my potential supervisor this week. As it would be a project for next year, he suggested that I get hold of a plant of interest and develop a callus culture as you suggested and then isolate and develop a sterile hairy root culture.

    The equipment that I have available was designed by a Professor in Korea who now mass produces ginseng roots. Quite an amasing feet considering they take 23-26years to reach maturity in the ground, where he has a couple of month turn over, hence why all these new energy drinks can contain it for so cheap. This is also why I am focusing on a root culture system, as the equipment is purposely designed for that. Although there is a degree of flexability there if a shoot culture presents itself.

    The first plant that my supervisor suggested off the top of his head was Derris. Despite that it is not the soundest ecologically, it was that he new the roots contained the compound rotenone and that I would get a result out of the experiment. Not to sure if I am that keen on this one though.

    Also as you sugessted Darklight, my supervisor has recommended that I find a plant that has been successfully cultured and try and tweek the methods, even to compare any potential yields through comparison of hairy root, suspension, etc.

    Obtuse, I was not sure where to place this thread, but you may be right that it should go in the chemistry section, but now I am not sure how to move it.

    Heretic, I like your suggestion too. I happen to be doing a small research project next semester, testing the anti-microbial properties of some various Australian natives, of which I will post up my results if I find anything of interest.

    Once again, thanks for your interest people, and please keep the suggestions coming. I would like to get a culture going this year so that I will have a sterile product to begin with next year

    peace

    cornilius maximus

    • Like 3

  13. Posted

    Hi All,

    This is not necessarily an ethno question, I just dont know where else to put it, BUT,

    Can anyone please suggest to me a plant worth investigating for an honors project which is capable of developing in suspension culture in order to extract secondary metabolites that may be worthy of publication? ( of course you will be mentioned in the acknowledgments section of the paper).

    I would prefer if the compound was contained in the roots, as this is the infrastructure most readily available to me.

    I have a few ideas in mind already, but I thought I may throw it out to the community to see if there was anything someone may have had in mind that they would like to see formally studied. At least to the best of my capabilities.

    peace

    cornilius maximus

  18. Posted

    Alienteaparty!

    You need to send me your address in a PM if you want to claim the print.

    I am posting out the others this morning.

    If you dont want it anymore let me know as there are other people who would like it as well.

    peace

    cornilius maximus

  22. Posted

    Hi All,

    As a token of my appreciation for this community I am giving away 5 prints FOR MICROSCOPY PURPOSES ONLY of Psilocybe cubensis - Amazonian to the first 5 people to respond to this thread who have more posts than I do, which is only around 50 or so.

    post-10814-0-87635200-1361846765_thumb.j

    I will post them all out together once they are claimed.

    peace

    cornilius maximus

    post-10814-0-87635200-1361846765_thumb.jpg

    post-10814-0-87635200-1361846765_thumb.jpg

    • Like 7

  25. Posted

    Hi All,

    I am currently in the market for a new pressure cooker as my old faithful is to small and doesnt seal very well anymore and was wondering if anyone can give me any ideas of what to and not to look for in this process.

    I use standard rectangle take-away containers for all my teks so I want to be able to fit at least 4 containers stacked.

    Any suggestions are delightfully welcomed

    peace

    cornilius maximus

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