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The Corroboree

Darklight

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Posts posted by Darklight


  1. On 12/5/2016 at 0:45 AM, Ex-Cess'es said:

    I've had no such experience so I can not offer any advice ,

    but I would love to know what the addition of trich to fertiliser and your garden in turn is good for ie perceived benefits . Just interested as I've never heard of such a thing , all though my knowledge runs thin but I'm learning or attempting to anyway 

     

     

    Trich and other microfungal/ bacterial components have, for me, increased yield and decreased disease in a few species.

     

    Also used them for increasing strike rate with unknown success- I don't have a controlled climate seedling house so am at the mercy of the elements which tend to be a bigger factor overall

     

    I've used a few commercial Trich blends with great success but it's getting exxy. There have been a range of other components from Azotobacter to fucked-if-I-can-remember-I-lost-the-labels. Trich is by far my favourite ingredient and has been the constant.

     

    Bunch of mechanisms, many of which I don't understand.

     

    A couple which stand out in my specific experience are:

    • I have a species with a strong tendency to die of fungal disease when stressed. When I foliar fertilise regularly with a Trich blend, the mortality rate for this species drops to almost 0%. Just an hypothesis, but I'm figuring the parts of the plant prone to environmental fungal infection are asymtomatic if infected with Trichoderma.
    • The effect wears off if I fail to fertilise for a month or so- it's not a one-off treatment, there are competitor organisms ( I've got a locally isolated slime mould in-vitro which has the most fascinating battles with Trichoderma- when it's cool the Trich takes over the substrate and the slime mould retreats or is eaten- when it warms up the Trich is eaten and the slime mould grows super rapidly- fascinating to watch, happens over hours ). My tentative conclusion is the Trich in the soil succumbs to the slime mould and similar at low temps, allowing the local bloody pestilence to attack my plants if overwhelming amounts of Trich isn't re-introduced regularly
    • Trich apparently evokes a mild stress response in plants, just enough to make them grow in response. No references, just something I've read

    Then there's the whole microfungi nutrient breakdown, saving plant roots some work in accessing them

     

    I would totally recommend trying this on at least some of your species- just a sample, no more than 30% of your stock on any species so you have some left if they don't like it

    • Like 2

  2. Anyone?

     

    Orbital shaker is on, I can easily run a scale-up on what I have, maybe make 100ml using malt extract + some sterile liquid fert components so it's not as much of a shock to the culture when I dilute it with non-sterile liquid nutes to spray

     

    Or does Trich need to be added as encapsulated spores ( ie commercial preparation ) directly into liquid nutes cos it only works as a foliar spray that way- throwing log phase sterile cultures into a non-sterile mix could potentially shock em to death?

     

    I can't even imagine an experiment around this which would be facile and robust


  3. 41 minutes ago, Gimli said:

     

    I have no problems with losing either tree to be honest.

     

    If anything, I should grow one up the massive fucking jacaranda tree out the front. Sick of that dropping its purple flowers all over my driveway every year

     

    Yeah kill the fucken thing. I like the flowers but if they go into yr gutters the tank water tastes foul

     

    Time lapse video it, it'll be like Megashark vs Giant Squid

     

    I have to trim the vine here every few weeks, but it's just used a hedge. If I left it the thing will grow up a tree and make a dash for the powerlines in six weeks during the growing season

     

    Not sure if it'd do that where you are, depends on how much water it can get

    • Like 1

  4. 13 hours ago, RonnySimulacrum said:

    We will be announcing this all tomorrow with the full list of Panels that will take place over the weekend

     

    Seriously brilliant work Ronny and crew.

     

    Outside the lab I am so completely flummoxed by anything more complex than the construction of a toasted sammidge.

     

    You all putting this together for the community is so absolutely brilliant I can't even...

     

    :worship:

    • Like 2

  5. Most of the things you've ever wondered about can be turned into an experiment so you can get results and stop wondering...

     

    Hell yes. Even if your results are inconclusive you get to rule out a bunch of stuff and know where you stand with regards to progress. Every step is a step forward.

     

    You often do not need as many of the shiny toys as you think- the best thing you bring is attitude, reliability, accuracy, resilience and resourcefulness. Most people have these. Framing your question so it produces valid data is the next step.

     

     

    You may have noticed that both Darklight and myself focus a lot on fun.

     

    I think that's because, for many of us, science is such a social process. It's fun, joyous, revealing, sharing, infuriating, frustrating and above all it's creative. I never learned about those aspects in school ( I was kicked out of HS science for asking too many questions and stayed away til my early 30s )

     

    Yep. Creative. Who'da thunk? I find the varying levels of experiment design, planning and execution similar in interplay to a Bach fugue- yes, the structure is important, but it's the contrapuntal nature of the different aspects of the process and the precision you bring to the work which evokes beauty and joy.

     

    Good science readily lends itself to networking, meeting more weird people with similar-but-different viewpoints on so many things who will teach you so much about your field and your worldview.

     

    I hear great research ideas every other day. Followed closely by all the reasons why that person isn't doing them.

     

    Yep me too. Those convos are so sad. Too many people underestimate their own talent and ability to contribute.

     

    Citizen-science projects like Fungimap and Atlas of Living Australia are prime examples of the scientific method being socially productive and fun- as well as making valuable contributions to scientific knowledge

     


  6. Seriously, youse all need to come to EGA. I do not use the word need lightly in this instance.

     

    90% of the people who would be reminding you why you need to come are fully immersed in actually setting it up. Drowning in it almost, just so you will have a deadset brilliant time

     

    I've been on the very outer edges of the org team and I have seen so much legendary epicness coming to the event- so many brilliant presenters and things and food and.... just you wait!

     

     

     

    • Like 1

  7. Ethnobotanical Research 101- starting from scratch

     

    Torsten and I are running a workshop on Laboratory Experiment Design at EGA 2017

     

    We want to share our love of citizen-science. To convince you all to partake in the formal, logical process that can answer so many of the phytochemical/ ethnotoanical questions you've asked over the years. Wonder no more! Act!

     

    Workshop's for beginners and wrinkly old lab-hands alike. Anyone, literally- anyone can design a simple, robust protocol which gives solid results and contributes to the sum of human knowledge. Those of you with extensive practical experience in experiment design are very welcome to share the ( sometimes bitter yet hilarious in hindsight ) fruit of your work with us huddled masses.

     

    It's not rocket surgery. Lab experiment design is a simple checklist, a bit of planning, some thorough checking and the resilience to simultaneously accept and critique the data as it falls.

     

    Carn, we all talk about experiments we'd like to see done. Or exceptions to established practices we've seen work. Shared variants or refinements of new teks.  Wanted to know why. Or wondered why the hell something didn't work out after we ( mostly ) followed the instructions.

     

    Workshop's interactive. Which means we need your input. Some of which can start here on the forums- reply with some pointers about your experiences or plans.

     

    During the workshop we'll welcome your thoughts, interjections, inspirations. Keep 'em coming, keep it moving

     

    Workshop's practice-based. Inasmuch as we're pointing at issues around design of theoretical experiments involving the legendary ethnobotanical Dragibus curiosa. Not sure what kind of experiment yet. Help us decide. A simple germination experiment?  Optimal fertiliser requirements?  The virtues of rhizobial inoulation? A cost/benefit comparison of propagation practices? Testing storage parameters for volatile compounds in the dry product?  Determining genetic markers for drought tolerance?  We'll settle the best questions on the day

     

    It'll be lighthearted. There *will* be lollies. Like all good laboratory-grade successes, some of them may be thrown at you, randomly. Some you must earn. Fate favours the prepared, apparently.

     

    It's serious business, experiment design-but that's no excuse not to have fun

     

    Bring your questions, your experience, your weird attitudes and your sense of humour.

    • Like 4

  8. Brugmansia. Always Brugmansia. Plant a few different cultivars.

     

    Not only do they attract heeeeeaps of bees, but when it's a drizzly day and the foraging's hard work your bees will still work the flowers, hanging out underneath them like umbrellas, waiting for it to be dry enough to fly back to the hive

     

    The resultant honey is not hallucinogenic, at least IME

    • Like 1

  9. 2 hours ago, shonman said:

    A lot of the new growth seemed to dry up and shrivel in the big aquarium 

    not sure why 

    now I regret putting all the plants in there..

     

    No idea about yr prob, sorry. I'd suspect bacterial, because of increased humidity and reduced air flow, but can't be sure I'm 100%.

     

    Is a good lesson to learn- when changing any parameter, keep some material back in it's original place. Always. Not only does it mean you have reserve material, but the remaining group acts as a control so you can compare.

     

    I'm not being unsympathetic at all- I recognise the temptation for a quick fix/ progress, and it's one I've succumbed to occasionally myself with the same results :/

     

    Goodonya for staying on top of it and making sure your babies didn't suffer too much for too long

    • Like 1

  10. IME unless you have super-dry facilities for it, it won't do well. Or even live.

     

    At least here, every time I have tried to deflask it, even the ambient moisture kills it. Something in the air maybe, bacterial perhaps, it does fine in TC so far with+90% ambient humidity

     

    Herbalistics sometimes has seed. Could be worth a shot that way


  11. 9 minutes ago, Master B said:

    Holy shit balls!!! Darklight.

    Its yours alright. 

    Nice work for the forum!

    Mate i have to double check? you do realize its for the graft in the very first two pictures? 

    But if your happy and all is sorted i will hook you up with a couple of other nice bits.

     

     

    Yea verily, I do know it's for the pictured graft of beauty

     

    You're the fulla who did the work. I think you just set a new forum record for stunning behaviour

    • Like 7
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