broken crow Posted June 28, 2002 is it possible ? any tips ? thanks Share this post Link to post Share on other sites
woof woof woof Posted June 28, 2002 however I am not able explain to you the exact techniques , Iknow that the answers is yES. If for example you introduce scraped hydrated spores on an agar solution in a petri dish...and with that mycelium inoculate rye grain jars. As soon as the rye grain jar is fully colonized , you should be able to colonize either MANY new rye jars by dropping a few colinized kernels (spawn) in the new ones , or you can just introduce the rye spawn to a sterilized substrate to inoculate. just a few examples. Be well Share this post Link to post Share on other sites
woof woof woof Posted June 28, 2002 Can you believe it... a triple post [This message has been edited by brian (edited 28 June 2002).] Share this post Link to post Share on other sites
woof woof woof Posted June 28, 2002 Can you believe it... a triple post [This message has been edited by brian (edited 28 June 2002).] Share this post Link to post Share on other sites
Guest Monkey Shines Posted June 28, 2002 This is from Fatguy's Agar Technique. "To inoculate with a spore print you should use an inoculation loop. This is a loop of wire with a handle. however you can make one of these with some wire that you can get at a hardware store. The wire should be able to be heated to red hot. - Remove the plastic or parafilm from the petri dishes and stack in piles of three. - Using a heat source, Heat the inoculation loop to red hot. - Open the agar dish and cool on the agar, then take a swipe of the spore print with the inoculation loop and spread the spores on the agar that you used to cool the inoculation loop. Replace the lid of the petri dish and seal with Parafilm or plastic wrap. - Re-heat the inoculation loop between every dish that you intend to inoculate. This will prevent cross contamination." I've never tried it but it seems good Share this post Link to post Share on other sites
