Paper chromatography

[Rev]

tdnntbh

Edited April 21, 2007 by Rev

[puffinstuf87]

 

quote:

---

1)Why would i want to use silica gel chrom. vs Paper chrom

---

Paper chromatography can usually only seperate very small amounts. Not really good for commercial applications.

 

quote:

---

2) Chrom paper is expensive. Like bacteriological grade agar vs food grade agar (10x less the price) - are plain filter papers (10x less the price) suitable for kitchen tek chromatography experiments

---

Filter paper,papers towels, tissue paper - try em all, its not like its a crippling expense. Ideally, your looking for the highest %age cellulose content, and youll probably have to experiment to find it.

Maybe try microscale column chromatography. Heres a couple of good Teks.

Expiremental Salvia Info

Chromatography Procedure

All the start up materials are recyclable, so enjoy!

[Rev]

tdnntbh

Edited April 21, 2007 by Rev

[puffinstuf87]

I dont have much of an idea about most of that stuff either - ive just picked up bits and peices. What are you using it for,if you dont mind me asking? If your trying to seperate something like a plant extract, id go with column chromatography unless your just analyzing the fractions. If your just researching, go paper or tlc. If you want a usuable product when your done, go column.

[Fractalhead]

Rev, how much chromatography paper do you get in those whatman packs? If carrying out analysis, use silica gel TLC plates over paper for superior resolution.

[Rev]

tdnntbh

Edited April 21, 2007 by Rev

[puffinstuf87]

Id use filter paper, for sure. It should do the job just fine. Filter paper of paper towels. I did paper towel chromatography in fourth grade - it took me tuil two years ago to realise i knew what chromatography was, and that i had done it. hahaha - good luck man.