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michaelangelica

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  1. Posted · Edited by michaelangelica

    Not all sages are the same

    "Investigation of in vitro Opioid Receptor Binding Activities of

    Some Turkish Salvia Species"

    http://www.acgpubs.o...NP-1008-306.pdf

    The most active species, S. sclarea and S. dichroantha need further investigation for the identification of the active components responsible for this activity.

    I often nibble ornamental sages; many are intensely bitter, indicating a high level of alkaloid(s) . I just need a portable GC. is there an app for that yet? :)

  3. Posted · Edited by michaelangelica

    It is possible that the flowers and especially

    resin of the plant is the most active

    very easy to grow

    PS It is Leonotis leonurus

    Antinociceptive, antiinflammatory and antidiabetic effects of Leonotis leonurus(L.) R. BR. [Lamiaceae] leaf aqueous extract in mice and rats.

    Ojewole J.A.O.

    Methods and Findings in Experimental and Clinical Pharmacology. 27 (4) (pp 257-264), 2005.

    AB The present study was undertaken to investigate the antinociceptive, antiinflammatory, and antidiabetic properties of the aqueous leaf extract of Leonotis leonurus (L.) R. BR. (Lamiaceae) in mice and rats, to scientifically appraise some of the plant's ethnomedical uses, and its safety and efficacy. The leaf powder of the plant was Soxhlet extracted with distilled water and used. The antinociceptive effect of the plant's extract was evaluated by the "hot-plate" and "acetic acid" test models of pain in mice, while the antiinflammatory and antidiabetic effects of the leaf extract were investigated in rats, using fresh egg albumin-induced paw edema, and streptozotocin (STZ)-induced diabetes mellitus, respectively. Morphine (MPN, 10 mg/kg i.p.), diclofenac (DIC, 100 mg/kg i.p.), and chlorpropamide (250 mg/kg p.o.) were used, respectively, as reference analgesic, antiinflammatory, and hypoglycemic agents for comparison. L. leonurus leaf aqueous extract (LLE, 50-800 mg/kg i.p.) produced dose-dependent and significant (p < 0.05-0.001) antinociceptive effects against thermally and chemically induced nociceptive pain stimuli in mice. LLE (50-800 mg/kg i.p.) also significantly (p < 0.05-0.001) inhibited fresh egg albumin-induced paw edema, and caused significant (p < 0.05-0.001) hypoglycemic effects in rats. It is suggested that the analgesic effects of LLE (50-800 mg/kg i.p.) may be peripherally and centrally mediated. The different flavonoids, diterpenoids, polyphenolics, and other chemical constituents of the plant may be involved in the observed antinociceptive, antiinflammatory, and antidiabetic effects of the plant's extract. However, the results of this experimental animal study suggest that the aqueous leaf extract of L. leonurus possesses antinociceptive, antiinflammatory, and hypoglycemic properties, and thus lend pharmacological credence to the suggested folkloric uses of the herb in the management and/or control of painful, arthritic, and other inflammatory conditions, as well as for adult-onset, type-2 diabetes mellitus in some communities of South Africa. 2005 Prous Science. All rights reserved.

  6. Posted

    Determination of four pyridine alkaloids from Tripterygium wilfordii Hook. f. in human plasma by high-performance liquid chromatography coupled with mass spectrometry.

    J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3516-22

    Authors: Cai MQ, Chen XH, He SW, OuYang XK, Jin MC

    Abstract

    A novel liquid chromatography-atmospheric-pressure chemical ionization-mass spectrometry (LC-APCI/MS) method was developed and validated for the simultaneous determination of four sesquiterpene pyridine alkaloids (wilfortrine, wilfordine, wilforgine and wilforine) in human plasma. The chromatographic separation was performed on a Shim-pack XR-ODS column using an ammonium acetate buffer solution-acetonitrile in a gradient program. The detection was achieved by an ion trap mass spectrometry in the positive selected ion monitoring (SIM) mode. The method utilized acetonitrile as protein precipitation solvent and followed by solid-phase extraction (SPE). Calibration curves were linear for the four alkaloids over the range of 0.5-100.0 μg/L with the limits of quantification of 0.5 μg/L, while the method exhibited the recovery of 86.5-98.6%, intra- and inter-day RSDs of less than 8.2% and 12.8%, respectively. Methodology was validated in line with the EU requirements (Commission Decision 2002/657/EC). Results of incurred samples demonstrated excellent reproducibility. To our knowledge, this is the first analytical method for simultaneous determination of the four sesquiterpene pyridine alkaloids in plasma. The method was applicable to clinical pharmaceutical research of alkaloids in rheumatoid arthritis volunteer patients after oral administrations.

    PMID: 21982911 [PubMed - indexed for MEDLINE]

    Triptolide (TPL) inhibits global transcription by inducing proteasome-dependent degradation of RNA polymerase II (Pol II).

    PLoS One. 2011;6(9):e23993

    Authors: Wang Y, Lu JJ, He L, Yu Q

    Abstract

    Triptolide (TPL), a key biologically active component of the Chinese medicinal herbTripterygium wilfordii Hook. f., has potent anti-inflammation and anti-cancer activities. Its anti-proliferative and pro-apoptotic effects have been reported to be related to the inhibition of Nuclear Factor κB (NF-κB) and Nuclear Factor of Activated T-cells (NFAT) mediated transcription and suppression of HSP70 expression. The direct targets and precise mechanisms that are responsible for the gene expression inhibition, however, remain unknown. Here, we report that TPL inhibits global gene transcription by inducing proteasome-dependent degradation of the largest subunit of RNA polymerase II (Rpb1) in cancer cells. In the presence of proteosome inhibitor MG132, TPL treatment causes hyperphosphorylation of Rpb1 by activation of upstream protein kinases such as Positive Transcription Elongation Factor b (P-TEFb) in a time and dose dependent manner. Also, we observe that short time incubation of TPL with cancer cells induces DNA damage. In conclusion, we propose a new mechanism of how TPL works in killing cancer. TPL inhibits global transcription in cancer cells by induction of phosphorylation and subsequent proteasome-dependent degradation of Rpb1 resulting in global gene transcription, which may explain the high potency of TPL in killing cancer.

    PMID: 21931633 [PubMed - indexed for MEDLINE]

  7. Posted

    High-throughput screening of chemical libraries for modulators of gene promoter activity of HLA-B2705: environmental pathogenesis and therapeutics of ankylosing spondylitis.

    J Rheumatol. 2011 Jun;38(6):1061-5

    Authors: Zhao L, Liu CH, Yu D

    Abstract

    OBJECTIVE: Ankylosing spondylitis (AS) is a highly heritable disease with HLA-B27 being the strongest susceptible gene. In order to survey the environmental triggers for arthritis development, we used a high-throughput technique to screen the effects of 12,264 chemicals on the HLA-B27 gene promoter.

    METHODS: Promoter reporter transfectants 293T-HLA-B27 and HeLa-HLA-B27 were tested using robotics with 12,264 chemicals. Chemicals that modulated HLA-B27 promoter activity > 150% or < 40% were selected for further evaluation of IC50/EC50 and cell viability.

    RESULTS: The primary screening using the 293T-HLA-B27 promoter reporter cell line yielded 5.1% hits that either suppressed (556 chemicals) or enhanced (68 chemicals) the HLA-B27 promoter activity. A secondary reconfirmation screening was carried out with these 624 candidates using HeLa-HLA-B27 promoter reporter cells under several different culture conditions. The yield of positive candidates was 130, of which 47 were derived from natural products. Based on the bio-information of those positive natural products, 21 chemicals were selected for analysis by dose-response IC50/EC50 experiments. Eight compounds showed potential pharmacological activities. Two suppressors are both derived from an herbal medicine (lei gong teng) that has been used for decades to treat immune diseases. The 6 activators all belonged to a group of chemicals known as flavonoids, widely distributed among dietary fruits and vegetables.

    CONCLUSION: Several common dietary products that contain certain flavonoids might be environmental risk factors for AS; the Chinese traditional herb lei gong teng might be a potential drug for patients who are HLA-B27-positive. These results provide new research directions for the pathogenesis and therapeutics of AS.

    PMID: 21459940 [PubMed - indexed for MEDLINE]

    • Like 1

  8. Posted

    Lobaline was used as nicotine replacement therapy.

    Ethnobotanically American Indians used it as well as tobacco and other herbs in their ceremonial peace pipes.

    All alkaloids seem to wear two faces, cure or kill.

    I have grow a few lobelias; The medicinal one to my memory was a large plant(1M+)with pretty scarlet red flowers You might still see it in Flower Catalogues (Chiltens?)

    Occasionally you might see it in nurseries. It needs a lot of water

  10. Posted

    Barringtonia Patent

    Novel Analgesic Compounds, Extracts Containing Same and Methods of Preparation

    United States Patent Application 20070270375 Kind Code: A1

    Various compounds obtained from plants of the Barringtonia species which are derived from Barringtoside A and Barringtoside C as precursor compounds which especially have an arabinopyranosyl substituent at the 21 position which may optionally be further substituted with benzoyl, dibenzoyl, methyl butanoyl, methyl butyryl or tigloyl at the 3 or 4 positions. Alternatively at the 21 position there is provided tigloyl, benzoyl or dibenzoyl substituents.

    http://www.freepatentsonline.com/y2007/0270375.html

    BTW

    A new pain relieving compound has recently been discovered in non-hot(as well as hot)Capsicum(s) spp.

  11. Posted

    Couldn't get photo's to work in the last post.

    I have more but I think it should be enough for ID.

    Sola

     

    Cytisus scoparius won't grow from cuttings. It should be a weed in SA; here it does not cope with my humidity. It needs European bees to polinate it.

    used medicinally (animals broken bones? JBL)

    It has anti-stress and moderate anxiolytic activity which may be due to its antioxidant effect

  12. Posted · Edited by michaelangelica

    I have just added to Wiki's Barringtonia acutangula entry.

    In the 20 or so reseach papers I could find there was no reference to the plant containing opioids.

    Does anyone have a reference (apart from the ABC's beat up) to the opioid content of the plant please?

    Perhaps someone with more organic chemistry could look at the list of chemiclas I made.

    http://en.wikipedia.org/wiki/Barringtonia_acutangula#Medicinal_Uses

  13. Posted

    I will be the first cult-leader to rip everyone off and then when they call me a scam artists, I will turn-around and say something like "so you have learnt nothing from this"?...

     

    LOL :)

    The problem with the big three intolerant religions is that they have so many good people working in, and for, them.

    This gives the evil people, in these fascist organizations, the cover to do harm and do evil things in the name of "god".

  15. Posted

    I don't see how you can blame religion on this one.

    Easy, aren't they responsible for most of the intolerance in the world.- Jewish, Islam, Christian.

    but seriously (as if I wasn't) I am referring to the happy-clappy, extreme right, Christian religions.

    have you ever visted the southern US states?

    or even Hillsong in Sydney where the collection plate is a stack of wireless Efpos machines?

    How is money got anything to do with the teachings of Jesus Christ?

    This really concerns me i see all our institutions, our social structures, under threat by the insane policies we have toward drug use. Let alone the incredible damage and violence done to families and people. On a $ cost alone basis, we should wise up-- with half the women in prison being there on drug charges.(Stupid- now we are going the Yank way and privatizing prisons!!- --probably run by drug barons?!). At $400+ a day, for room and board, + mega$-legals++ that is insane. The Yanks have a huge drug-prison population, mostly of minorities.

    We don't have to be as stupid or as venal as the yanks ALL the time.

  16. Posted

    a good example is salvia D, small doses can be enjoyed where as large doses especially for a first timer arent always so fun, im sure everyones typed in "worst salvia trip ever" into youtube hahaha

    Large doses can be enjoyed by some people for sal d but im just interested in the small side of things in this thread.

     

    People often dont take it in the traditional way.

    ie 20-30 leaves juiced (fresh)

    i lost a huge plant in an unseasonable frost, that i smuggled in from B&T

    any ideas on where to get another? I don't want to push my luck a second time. (PM me if you wish)

  18. Posted · Edited by michaelangelica

    O.K., the thing is there's no fence to p[aint white. The mirrors would be attatched to the eve of the house roof and reflect back from there. Mirrors are my only real option, just want to find out if they reflect sunlight true or wheather they absorb some light spectrums. Also, I figure using a convex mirror will avoid hot spots.

     

    I have been mucking about with mirrors for a year or two in my shady garden.

    I've used reflective gold inserts from smoked salmon, mirrors from the house wrecker, mirror stickers, stainless steel mirror $8 from Bunnings, mirrors from garage sales. I think all i have managed to do it collect more junk and confuse a few plants.

    I really need a mirror that is heliotropic.

  19. Posted · Edited by michaelangelica

    (Ps moderator how do I correct spelling in title Portuguese?

    ISTM that the Oz drug laws are evil; dangerous, deadly and dopey. They also corrupt all levels of our society; destroying our institutions of law and justice.

    They make a lot of people rich, at the expense of the suffering and destruction of many lives.

    It is interesting to see that the Portuguese experiment is going so much better with much less harm than the crazy Yank system

    In the face of a growing number of deaths and cases of HIV linked to drug abuse, the Portuguese government in 2001 tried a new tack to get a handle on the problem—it decriminalized the use and possession of heroin, cocaine, marijuana, LSD and other illicit street drugs. The theory: focusing on treatment and prevention instead of jailing users would decrease the number of deaths and infections.

    Five years later, the number of deaths from street drug overdoses dropped from around 400 to 290 annually, and the number of new HIV cases caused by using dirty needles to inject heroin, cocaine and other illegal substances plummeted from nearly 1,400 in 2000 to about 400 in 2006, according to a report released recently by the Cato Institute, a Washington, D.C, libertarian think tank.

    http://www.scientificamerican.com/article.cfm?id=portugal-drug-decriminalization

    Portugal's drug laws

    Ten years ago Portugal had one of the worst drug problems in Europe. Heroin use was out of control and the rate of HIV infections in drug users became a humanitarian crisis. So what did Portugal do? They decriminalised all personal drug use in that country, crack, heroin, LSD, you name it. Drugs are still illegal, but it's no longer a crime to use them. Instead of jail, users and addicts are offered treatment and education. In this Health Report special feature Nicky Phillips looks at how Portugal transformed their drug laws and whether this worked.

    http://www.abc.net.au/rn/healthreport/stories/2009/2661510.htm

     

    It's been eight years since Portugal decriminalised drug use, so why the sudden interest?

    Well a recent review of Portugal's drug laws by the CATO Institute, that's a think-tank, has sparked significant attention because the report found decriminalisation a success, and it suggests other countries with drug problems give it a go

     

     

    In 2001, the Globe and Mail reported that a poll found that 47% of Canadians agreed with the statement, "The use of marijuana should be legalized" in 2000, compared to 26% in 197

    http://en.wikipedia.org/wiki/Drug_liberalization#Canada

    A lot of the opposition in Oz seems to be from the loonier Christian right ---those crazy, filthy rich, evil Yank cults.

    Perhaps they are so rich because they are funded by the drug lords; who certainly don't want to see the law changed.

    Strange bedfellows.

  22. Posted

    The great banana hoax

    L Bozzetti Jr, S Goldsmith, JT … - American Journal of …, 1967 - Am Psychiatric Assoc

    ... The recent practice of smoking dried banana scrapings to achieve a “psychedelic experi- ence”

    led the authors to investigate the hal- lucinogenic properties of bananadine, or “mellow yellow.”

    They conclude that the “active ingredient” in bananadine is the psychic suggestibility ...

    Such creativity!

    From Tales of Beatnik Glory, Volume 3: The Psychedelicatessin

    E Sanders - The Review of Contemporary Fiction, 1999 - questia.com

    ... As a cover the Feds opened a business called Bananadine, Ltd. The place had been a pizzeria

    and now the floor-to-ceiling bank of steel-doored ovens was used for the baking and curing of

    Bananadine. Business expanded so quickly at Bananadine, Ltd. ... At Bananadine, Ltd. ...

    http://www.springerlink.com/content/02060rj354480858/

    http://resources.metapress.com/pdf-preview.axd?code=02060rj354480858&size=largest

    Many herbs that are said to help depression (like borage) contain large amounts of Phosphorus. Bananas contain this + three sugars and some tryptophan the precursor of seratonin ( also a precursor to Niacin essential for nerve-mylin-sheaths)

    The disorders fructose malabsorption and lactose intolerance causes improper absorption of tryptophan in the intestine, reduced levels of tryptophan in the blood[12] and depression
    -wiki

    Many non-European people have lactose intolerance; something the Europeans have only developed in the last 10,000 years.

  23. Posted

    px.gif

    You would't think a red would be that hard to hybridize.

    This guy seems to have a lage range of over-priced ones

    http://www.sacredgardenfrangipanis.com/angels-trumpets/index.php

    brugmansia_hot_pink_005.jpgbrugmansia_red_hot_pink_004.jpg

    This guy is a wholesaler

    http://www.plantasy.com.au/temp/plantasy_www/showplantdetails.php?PlantID=24

    I have several plants from seed that should flower soon (USA Hybrids from Chiltern).

    I have white, double white, Apricot, gold and pink; so I am hoping for something new. The plants were surprisingly easy from seed.I am also looking for good fragrance. The double white seems to be the best so far.

  25. Posted · Edited by michaelangelica

    dont think there is a b fragrans....nothing on google either....?

    t s t .

     

    Yes, a seniors moment, sorry

    Brugmansia suaveolens (=agreeable/sweet scented)

    I think there is a book, just on the Brugmansias, which may give more accurate botany

    B and T World Seeds' reference number: 400686

    USDA average, annual, minimum temperature Zone:9

    Type of plant - shrub

    Flower: WHITE (YELLOW / PINK) noct. frag. trumpets to 20-30 cm.

    Foliage: ovate to narr.-elliptic, glab., entire

    Height in meters: 2.0(5.0)

    Parts of Brugmansia suaveolens are considered toxic.

    Synonyms (alternative names) for Brugmansia suaveolens: Datura gardneri err, Datura suaveolens err,

    Common names for Brugmansia suaveolens: (tox:scopolamine etc. tropane alkaloids), Maikoa, med:mydriatic effect from eye contact,~6 days),

     

    PS

    Dr. Duke's

    Phytochemical and Ethnobotanical Databases

    Chemicals in: Brugmansia amesianum (SCHULTES) D'ARCY (Solanaceae) -- Culebra

    Chemicals

    ALKALOIDS Leaf 3,000 ppm; DUKE1992A Stem 3,000 ppm; DUKE1992A

    ATROPINE Plant: DUKE1992A

    SCOPOLAMINE Plant: DUKE1992A

    Its hard to find a good chemical analysis of the plant. It seems however that the plant can change this at will,. A possible cause of poisoning?- "You bite me; I bite back" ?

    Abstract Brugmansia suaveolens (Solanaceae) contains tropane alkaloids (TAs), which can act as chemical defenses. Selective pressures might modulate the allocation of alkaloids within the plant, as postulated by optimal-defense theory.

    By tracing scopolamine, the most abundant TA in this species, we found that scopolamine in an artificial diet, in concentrations similar to those in leaves of B. suaveolens, increased mortality and prolonged developmental time of the larvae of the generalist noctuid moth Spodoptera frugiperda.

    A diet of undamaged leaves of B. suaveolens also showed a large negative effect on the growth of larvae of S. frugiperda compared to a diet of leaves of Ricinus communis, a species that did not have negative effects on this moth; more valuable plant parts, such as young leaves, flowers, and unripe fruits with seeds, have higher scopolamine concentrations than other tissues; leaves of B. suaveolens increase their content of scopolamine after artificial damage.

    The highest induction was found 24 hr after the damage, and after that, scopolamine content decreased to constitutive levels.

    This increase represented a cost, because in another experiment, a treatment with methyl jasmonate, an elicitor hormone, increased scopolamine production 9.5-fold and decreased leaf growth 2.3-fold; a diet of artificially damaged leaves of B. suaveolens showed a negative effect on the growth of larvae of S. furgiperda compared to undamaged leaves, suggesting that damage by herbivores induces resistance.

    http://www.springerlink.com/content/v3182j712717h8j6/
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