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mimzy

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Posts posted by mimzy

  11. Posted

    The first germinated with all the others, but stayed very skinny. There is also a lot more white tissue relative to green tissue. i assume the white tissue becomes more woody and root-like and the green part forms the plant we see on the surface.

    The second one doesn't have two areoles form what I can see, but there is a definite divide/line in the seedling. Looks like a conjoined twin almost.

    Haha well said Prier, I don't intend on counting my chickens any time soon - but they are interesting though. I also have a bunch of abnormally small ones (you can see one in the second photo)

  12. Posted

    I recently sowed my first batch of L. williamsii seeds and was astonished at the diversity in seedling morphology and growth rate.

    In particular, these two seedlings are quite peculiar and I'm trying to figure out if they are genetic mutants or simply different growth morphologies.

    This one is far more elongated than the others

    Rsz 1img 0875

    This one has two segments

    Rsz Img 0878

    Any thoughts?

  16. Posted

    One my first go at potting up my cacti I used a mix of approximately 70/30 potting mix and perlite. Everything seemed fine to start, but then I started to loose a few plants. I discovered that when I thought I was watering/fertilising, I actually wasn't. The perlite had made my soil so hydrophobic that the water merely soaked the first 1-2mm and ran down the edges of the pot, leaving the root system very very dry. Now I have to bottom soak each plant just to make sure it gets a drink! Definitely switching to torstens 70/30 potting mix and river sand medium for the next re-potting.

  17. Posted

    Haha tell me about it Shonan! I'm over mine, and I'm only 9 months in. I like the tissue culture idea, although I'm not sure plant cells like forming a monoculture as readily as animal cells.

  18. Posted

    I'm reticent to say it's impossible, but it would be very difficult. You're first major hurdle would be to identify the gene(s) responsible for your medicinal compounds. Very rarely is a medicinal compound a one gene show, especially in plants - most are a side product of complex biochemical pathway involving many genes and gene regulators.

    I imagine that it would be easier to generate a plasmid with the genes for the major protein players. You could insert this plasmid into a host E. coli strain relatively easily for replication and transcription. Maybe if you provide the bacteria with the base compounds of the biochemical pathway you would get your end product. There are many variables at play, indeed this would probably represent 2-3 years work and a PhD :)

    • Like 4

  19. Posted

    I can't go around calling out newbies just yet, but seriously... there is so much information available on this site, let alone the entire internet, to get you from point A (where you are) to point B (where you want to be). Connect the dots, and be patient... not everything in this world comes in a pill you can swallow.

    • Like 1

  20. Posted

    Using aseptic techniques it is possible to inoculate the culture jar without contamination, however from time to time it does occur. In most cases, if the jar is contaminated by mold or bacteria you will be able to see the contaminating colonies and discard the culture.

  24. Posted

    It's L williamsii. I spray my grafts with GA3 and it increases the growth rate, but also causes the ribs to pop out like that. It's actually only 8 ribs, it's just spiraling. Here's a picture of it when I first grafted it.

    attachicon.gif9-1.jpg

    @ bullit: I suspect it's rust rot, but it isn't soft. I wanted to get a few other opinions. It's grown indoors under T5 lighting (during the winter) and it was outside for a while at the beggining of it's life. I have never had issues with rot before on any of my other GA3 sprayed grafted lophs.

    Thanks for explaining. What order of increased growth rate do you achieve by using GA3? Double, triple?

  25. Posted

    Hi guys.

    Not the greatest of offerings here, but I recently found a Persian store stocking wild rue seeds and bought a big pack. I haven't sown any yet, so I cant give you a 100% guarantee they are still fertile... but as far as I know they are fine to grow bought like this. I have so much more than I will ever need, so I'll be sending them out to whomever wants them.

    Cheers,

    Mimz

    • Like 2
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