Jump to content
The Corroboree

mimzy

Members2
 View Profile  See their activity

  • Content count

    264

  • Joined

  • Last visited

  • Days Won

    1

Posts posted by mimzy

  3. Posted

    From a hobby long ago.... Includes bridgesii, pachanoi sp. and some interesting crosses/ varieties - Yowie, Penis. Maybe some other variants psycho0? I haven't spotted. Would be great stock to start a collection or propagate out. Easily 10m of cactus. 

     

    Pictures:
    https://ibb.co/RjMV45j
    https://ibb.co/WP8xB89
    https://ibb.co/YXL49qL
    https://ibb.co/PwMMHkP

     

    Located Blue Mountains (mid). 

     

    Make an offer, must collect.  

  6. Posted

    You shouldn't find Trich sp. inside stipe or cap tissue, but you will regularly find pseudomonas which can be easily cleaned up with amp. I also wipe down my specimens with h2o2 and tear them open rather than cutting. This is important because often contaminants can be introduced into the the tissue by the knife as it passes through the 'dirty' surface layer.

     

    Also, where is the contamination popping up? If at the inoculation site then this would be consistent with specimen contam, but if elsewhere then maybe your plate wasn't sealed?

     

    With love,

     

    Mimz

    • Like 1

  9. Posted

    The only way I know of recovering a strain from mites is to slow freeze your culture in 15% glycerol /water. Freezing will kill the mites but your cultures should be ok. I was lucky most of my plates were sealed with parafin tape and avoided disaster. Any new clones from fruit bodies collected el-natural go straight in isolated eskie until I can confirm they are ok.

    It leaves in awe of how finely balanced our ecosystems are; in the lab its always one victor over another... whether thats mold, mites or moths.

    • Like 1

  14. Posted

    (1) For the love of all things good in this world, do not store more grain than you have to; or at least store it properly. For most of my commercial cultures I use organic wheat grain. Some time ago I bought 30kg sack, thinking I would cut down on costs by buying in bulk. The first sign of trouble was a few pantry moths flying around the lab. No problems I thought, I'll leave the sack out in the sun for the day to kill off the weevils and store it in another room. Well, it turns out the sack was infested, and with the humid warm conditions of late summer, I soon had 100's of moths in all my rooms, in all my medias, in all my grains, in all my supplements, in all my everything!

    (2) Put your agar plates in the fridge as soon as they have set. Leaving agar plates out on the bench for extended periods of time gives mites (YES MITES) a chance to crawl in, contaminate your plates with asper. sp and/ or lay eggs that will ruin your culture later. Don't try to save your plates; freeze them to kill the mites and throw away. Again, this time of year is particularly bad with high humidity.

    (3) If you can avoid it, don't have any timber in your clean space- given enough time and moisture you can have a mold city growing under benches, which you wont know about, absolutely loading the air with spores. Keep it stainless or plastic.

    Learn from my mistakes fellow mycologists.

    With love,

    Mimz

    • Like 8

  23. Posted

    Hi folk,

    Due to a disaster in my laboratory (my fridge froze over) I'm currently looking for following strains;

    Pholiota nameko

    Pleurotus citrinopileatus (Golden oyster)

    Pleurotus pulmonarius (Blue oyster)

    Pleurotus djamor (Pink oyster)

    If anyone can help me out with some agar cultures or prints, I will be eternally grateful. I still have most of my strains to trade of course.

    Cheers,

    Mimz

×