TheMooseZeus Posted March 13 Share Posted March 13 After moving house I started to notice spots of mould on my agar plates... My technique and set up had been great at my previous spot but decided to work on it incase I had become complacent... No luck Moved my setup into the bathroom (toilet separate) after a bleach clean... These are the latest... FFS!! Quote Link to comment Share on other sites More sharing options...
saguaro Posted March 18 Share Posted March 18 are these plates sealed? Quote Link to comment Share on other sites More sharing options...
TheMooseZeus Posted March 18 Author Share Posted March 18 2 hours ago, saguaro said: are these plates sealed? Ziplock bags are sterile out of the box so they make for a great breathable parafilm alternative. I have been using them for years. Im very small scale so I don't get through too many and use used bags in propagating plants. Easier than parafilming glass plates thats for sure Quote Link to comment Share on other sites More sharing options...
saguaro Posted March 19 Share Posted March 19 (edited) Ziplock bags are definitely not sterile, although I'm not questioning whether or not it has worked for you in the past. If there is a high level of airbourne mould, you are using a still air box and glass plates while effectively not sealing them, there are a lot of vectors of contamination. You might need to use a HEPA filter / laminar flow. Are you PCing the plates between use? If so how are you transferring them to your work bench from the PC? Edited March 19 by saguaro q Quote Link to comment Share on other sites More sharing options...
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