Does this look like oyster myc?

[Black Rainbow]

First time trying to clone oyster tissue on agar. Does this look right? About four weeks in and the only dish of ~8 with any growth on it.
 

 

[87_botanist]

I can enlarge the picture so I cant tell bout four weeks I would expect the plate to be well and truly covered

[Black Rainbow]

Sorry about that, I'll get a better image.

That was my understanding, too. Is there something that could be inhibiting growth?

 

I'm wondering if the problem was the environment of the dish, the flesh I cloned or my malt extract and agar medium. Any suggestions for how I might get a better understanding of where I went wrong?

[Black Rainbow]

[Berengar]

This does not look like pleurotus to me at all, yeasts are more likely. Can you please describe your proccess and the conditions the dish is kept in?

[Black Rainbow]

I double boiled a combination of 250ml water, 1½ tsp agar and 1 ½ tsp malt extract in jar with lid slightly ajar until everything dissolved. When the jar was cool I added 2ml of peroxide. Then I poured a few cm of this mixture into each of my plastic containers, before taking small pieces of flesh from inside my pleurotus specimen, placing a piece in each container.

 

Since then, the containers have sat inside a sterilised glove box in a darkened room at a temperature between ~15 and 22 degrees. The container pictured is the only one with any growth.

Edited March 1, 2018 by Wile E. Peyote

[Berengar]

Remove peroxide from your recipe. If you want to use peroxide, then use it to dip the tissue sample into it for a second before placing it on agar. And try with a different mushroom, maybe the one you used was dead for some reason.

Oysters are usually extremely aggressive, it should grow on pure agar, carboard, pasta...

It's never a good idea to use peroxide IMO, even though it may seem like it would be be. It usually does more damage to mushroom mycelium than it prevents molds from growing.

[Black Rainbow]

Thanks I'll give that a go!