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Showing results for tags 'cloning'.
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Hi all, When cloning mushrooms, which flush is preferred to take clones from? I have an average understanding of genetics but I can't understand why cloning from a mushroom fruit would promote growth similar to the cloned mushroom- surely you are cloning the whole organism ie. the mutation is in the organism not just the fruiting body? Assuming I have this all wrong, which flush should I clone from? Second flushes are usually a lot more prolific but 3+ are big chonkers in my limited experience. Cheers all:) (Open to spore and culture trades )
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Hi all, The content of this post is going to be blaringly obvious to most people but I thought it was worth sharing my method of cloning B. caapi. I worry about my caapi for no particular reason so I take cuttings when I can. I had one not doing too good in early winter so I turned it into a number of cuttings. I took sections of 15 - 20cm, soaked the ends in Clonex (Hardwood 8g/L IBA) for approx. 5 mins and stuck them in a small plastic pot of moistened potting mix. I then put this pot into a round take away container and taped a ziplock bag around the whole thing to ensure humidity. I also had one cutting in a tube stock put hanging up in a ziplock bag. This worked great! The first thing I noticed was fungi growing from the tips of the cuttings but this quickly died out. As roots started to grow out of the pot and into the bottom container and leaves appeared I started to cut holes into the plastic bag to hardening them off. This was inside with no direct sun during a Sydney winter. Eventually I removed the bag all together and watered with a dilute seaweed fertiliser (OCP Eco-seaweed) to encourage root growth. Keeping the plants away from direct sun. After most of the plants had 2 sets of leaves I separated them into tube stock and they went outside! They looks great! Throughout the whole experiment I realised the best cuttings were the largest. Fastest to root and put out leaves etc Cheers all!! God bless rooting hormone
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Hi, I've recently become a registered sole trader and in support of the community who helped supply me with much needed cultures, I'd like to return the favor. This offer is aimed at amateur mycologists or those interested in mycology without higher end equipment. The Offer: I'd like to offer a cloning (or isolation) service to anyone looking to put a species onto agar. I'll get this out of the way first, this offer is applicable only for species legal to possess live. If you have spores or a wild specimen that you'd like to get onto agar, or need a clone or isolation of an existing culture, PM me or contact me via email at [email protected]. What I ask for the service is that shipping costs be covered by the purchaser and $12.00 to cover the cost of materials for the clone. I remember exactly what it was like to have to make do for equipment, and while it worked well in some situations, there's often something that just doesn't go correctly. While mycology was hobby for me, I was confined to a small glove box working with makeshift conditions that often ended in contaminations . A few of thousand dollars later, and I've got a sterile, comfortable workplace. The setback of contamination was always a drain on the fun of the hobby for me, especially when it came down to hoping that I didn't stir up too much air in the glovebox, or when attempting to do anything with liquids at all in a small glove box. I want others to be able to enjoy mycology as much as I do, and having a live culture gives much more power when inoculating then trying to start from spores that are hopefully sterile. Kind Regards, David.