Alchemist
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Posts posted by Alchemist
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Help, every time I click on my profile I get :[#10245] You are not permitted to view member profiles. Also all my 16 posts don't exist. Just after posting this, now clicking on my pic seems to work....?
Cheers
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Someone ought to try extracting and purifying other common salvia species such as splendens. Ok it may not be active smoked, but perhaps a large dose of the pure compound is.
Can you do it mr salvinorin!
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Yeah I grew my own but it tastes revolting, so I no longer use it. Its so revolting that I think it is unnatural for humans to drink it.
It makes you want to throw up. Silly books say its because it detoxifies the body so quickly, but ipecac syrup does the same and purifies nothing!
I'm now using recepies from Norman Walkers 'Raw vegetable Juices' Book. (they guy lived to well over 105 years)
I found a new plastic juicer for a cheap price of 65 dollars. Good for juicing leafy stuff like spinach, and lettuce and wheatgrass.
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G'day folks, due to some health problems, I've decided to try wheatgrass juice for a year. I already have a centrifugal juicer, but it is next to useless for juicing grass. To my horror, a hand powered wheatgrass juicer sold at a juice shop costs 120 dollars. I cant afford to spend this much so I'm wondering if anyone out there has a second hand one for a reasonable price, or knows where I can get one.
Email: [email protected]
Cheers :confused:
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Resolution
For a detector to distinguish between ions differing by one unit eg NH3+ and CH4+ (m=16 and 17) the instruments resolution should be about 16. If we are looking at masses of 220 and 221, the resolution jumps to about 221.
Analysers
The original and most massive MS is the magnetic sector analyser. These are very good for high resolution analysis. For example C2H4+ and CH2N+ both have a mass of 28 but their exact mass is 28.0313 and 28.0187. Resolution required is 2200.
Quadrupole
Very compact, less expensive and more rugged, low scan times, by far the most common.
In a magnetic sector analyser ions are dispersed simultaneously like a diffraction grating. In contraast the quadrupole is analagous to variable narrow band filter. At any set of operating conditions, only ions with a small m/z (mass to charge) ratio are transmitted, the others are blocked.So it can be thought of as a mass filter rather than an analyser.
Most units can resolve ions differing by one unit upto over 3000 m/z ratio.
Ion Trap
The ion trap analyser is a device in which gaseous ions can be formed and confined by extrended periods.RF energy voltage is scanned and ions of specific m/z ratios destabilize and enter a detector.
They are cheaper than quadrupole and can resolve one mass unit for mass ranges of 500-1000.
They are apparently very good analyzers. With fourier transform, they are excellent instruments capable of a mass range of 12-2000 with a resolution of 50k-760k.
What to use them for
High res MS can give you exact MW down to a few decimal places.
Scructural information from the fragmentation (must have a lot of experience to be effective, and it is impossible for complex molecules).
Compound identification using a library.
Quantitative analysis. This requires a standard of the same compound being tested, or one which is very similar. Select one or more m/z peaks and the area under them will be proportional to their concentration. So for DMT and MeO-DMT, a standard of DMT may be used for both, if an appropriate peak is chosen.
If you need more information, call labs or universities and ask to speak to the mass spectrometrist. Im no expert and this is all I know.
[This message has been edited by Alchemist (edited 23 July 2002).]
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I do hope this has not been posted already, but a seach on the site came up negative.
Check out http://thomas.munro.com/
for some possibly interesting info and links
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Found some info on reflexa. First it seems that its toxic principle are simple water soluble nitrate salts which are only poisonous in massive quantities. An acetone or ethanol extract won't have these.
Also it seems to contain a lot more salviarin than S.splendens does, which has predominantly splendidin. Still at 1.16mg/g it is still quite low.
My guess is that Thelema will live to see another uneventful day, but a fair amount of 10x (using acetone) extract smoked is worth a try.
Have a read for yourselves.
The toxic properties of Salvia reflexa.
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Williams, Cecil H.; Hines, H. J. G Australian Vet. J. (1940),16 14-20.
Salvia reflexa (wild mint or mint weed), known in Queensland and N. S. Wales as a plant poisonous to stock, was studied to discover the toxic principle.
Tests of the dried plant for saponin and for cyanogenetic and other toxic glycosides were neg., but ppts. were obtained with all the usual alkaloidal reagents. The substance responsible for these ppts. is extractable by refluxing with acetone and was shown to be choline.
It is present, however, to only 0.05% and is probably not a factor in the poisoning. The dried plant material was free of nitrite
but contained 5% KNO3. Maceration of the plant material for 20 hrs. with water and a little CHCl3 reduced 40% of the nitrate to
nitrite. In a feeding test 800 g. of the material was macerated in 5 l. H2O for 40 hrs. and the liquid expressed. About 0.75 kg. of the marc was fed to a sheep without ill effects, but 3 l. of the expressed liquid was fatal to another sheep in about 6 hrs.
The O2-carrying capacity of the blood was reduced from 16 to 4.53 ml. O2 per 100 ml. of blood at death. This is interpreted
as indicating conversion of 72% of the hemoglobin to methemoglobin by the action of the nitrite. Conversion of nitrate to nitrite may occur through the agency of enzymes from the plant or the animal. Since about 10% of the nitrate fed to sheep is converted to nitrite in the animal
body (Seekles and Sjollema, C. A. 26, 3842) 30-60 g. of nitrate should
constitute a toxic dose for a 60-lb. sheep. Eighty g. of KNO3 killed a sheep in 48 hrs., 71.5% of the hemoglobin having been converted to methemoglobin. The plasma of the abnormal blood gave a strong test
for nitrite. However, the juice from 800 g. plant material, corresponding to only 25 g. KNO3, was fatal to a 45-lb. sheep in 21 hrs. Thus the nitrate in the plant acts similarly to nitrate by itself,
but the toxic dose can be much smaller. The accumulation of nitrates in plants, the toxic action of nitrates and nitrites
and nitrite poisoning under field conditions are reviewed.
Ordinarily herbage contg. large amts. of inorg. N is unpalatable to stock and the large dose necessary for toxic effects will not be ingested.
Chemical analysis
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Nieto, Matias; Gallardo V., OscarRossomando, Pedro C.; Tonn,Carlos E. 8-Hydroxysalviarin and 7,8-didehydrorhyacophiline, two new diterpenes from Salvia reflexa.
J. Nat. Prod. (1996), 59(9), 880-882.
Extraction and Isolation. The dried aerial parts (2.4 kg) of S. reflexa were extracted twice with Me2CO at room temperature for 10 days, and the resulting residue (130 g) was chromatographed over Si gel using a hexane-CHCl3 gradient. After several column chromatography separations, salviarin (1) (2.85 g), 6-hydroxysalviarin (2) (0.043 g),
8-hydroxysalviarin (3) (0.310 g), and 7,8-didehydrorhyacophiline (5)(0.135 g) were obtained.
The dried roots (75 g) of S. reflexa were triturated and extracted with boiling Me2CO. The residue (8 g) was purified over Si gel using a hexane-EtOAc gradient, and after several column chromatography separations, oleanolic acid (0.980 g) and -sitosterol (0.080 g) were obtained.
[This message has been edited by Alchemist (edited 03 May 2002).]
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[This message has been edited by Alchemist (edited 30 April 2002).]
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[This message has been edited by Alchemist (edited 30 April 2002).]
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Originally posted by blog:
NO2 is nitric oxide, you'd be coughing up blood if you did manage such an effort as breathing the concentrated gas. But ofcourse you meant N2O.
I think she meant CH3CH2CH2CH3 as well!
Double trouble!
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[This message has been edited by Alchemist (edited 05 March 2002).]
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[This message has been edited by Alchemist (edited 05 March 2002).]
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[This message has been edited by Alchemist (edited 05 March 2002).]
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[This message has been edited by Alchemist (edited 05 March 2002).]
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[This message has been edited by Alchemist (edited 05 March 2002).]
I can't edit profile
in Site & Forum Matters
Posted
I've been on here for 9 years and somehow my 16 previous posts are non existent. What happened to them Torsten?