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Everything posted by enohpi-zeekim
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Sky blue mushroom ( found @ kuriositas.com ). Botanical name- Entoloma hochstetteri
enohpi-zeekim posted a gallery image in Member's Gallery
From the album: My mycology.
I found this cool little shroom online today, a sky blue mushroom. Thought to be found only in NZ and parts of India. Pigment is Azulene blue, or , which is also found in other plants in nature such as seaweed or algae. I just thought it was a awesome mushroom and wondered if anyone had tried to grow one of these for a display type hobby humidity terrarium? I would be interested if anyone knows any interesting facts about it from their own experience. Is there anythungi like it in Oz?© Kuriositas.com
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Lophora williamsii, first of my australian collection.
enohpi-zeekim posted a gallery image in Member's Gallery
From the album: My lophora willi
Long awaited new addition to my garden collection. Thanks SAB. Going to be a few years before I have the collection i once had when I left NZ. Oh the memories and all the spiny repotting year after year My old cacti collection was ( 40 trichocereus pachanoi between pup size and five foot, 10 trichocereus scopulicola 2-4 foot +pups and a couple lophora williamsii and two grafted to pach's. and a couple agaves...and a garden gnome. I left my babies behind. Was my pride and joy. Well here's to many more in my new and soon to be improved australian version. One thing is Australia has the climate for an potentially amazing collection with all the species that I could never either get or never get to grow. Here's to you Aussie and all your amazing plants, and the people who are wiling to share and give advice. This is the little notch I was waiting for. It's all fun and games now, I hate feeling like I'm missing something in my garden life. -
No change but still green, more than I can say for the other sample!
enohpi-zeekim commented on enohpi-zeekim's gallery image in Member's Gallery
Was a shot in the dark really I was only going on what I thought to be right as far as what my idea of how it's done. Well since this, I decided to get a little more interested and actually research it lol. Still haven't found the absolute right way to do it because there is a broad range of variables with each different plant. Sort of goes without saying but as far as culturing went I assumed it would be fairly relative to plants. So I spotted a contaminant in the jar so out it went. The next time I'm actually going to ask an expert for advice. Unfortunately I think it's going to mean more money suck. Thanks for your interest though. -
No change but still green, more than I can say for the other sample!
enohpi-zeekim commented on enohpi-zeekim's gallery image in Member's Gallery
Just that when you get good at it I guess, you can turn a small part of a plant into many and is nice to just try what can be done. My hope was to just see if I could do it. Hope to one day be good enough at it to grow more hard to get plants that I can't get often. The main purpose is dependent on what you aim to achieve, some silvercuturalists use it to get many trees of the exact genetic quality. It's the same for collectors of exotic or rare plants. Breeding good genetics then cloning from cell culture. I don't know what the percentage of plant they use but I had thought in perfect conditions a seed could be divided many times to become a forest or a plantation. Mainly it's interesting and I like to learn. -
No change but still green, more than I can say for the other sample!
enohpi-zeekim posted a gallery image in Member's Gallery
From the album: Experimental culturing. Fail.
I didn't have high hopes for this working but so far still green. Better than what I can say for my control which just had the MEA dextrose, indole acetic/ naphthalene acetic and GA3 . No iodine and no seaweed nutrient. But neither were dipped in bleach for contaminants or treated with ethanol. So I think it's just a matter of time until they die. -
Experimental culturing. Fail.
Images added to a gallery album owned by enohpi-zeekim in Member's Gallery
Tissue culture sample from & incl. leaf, (Venus Fly Trap)!! First try @ this no previous research on plant tissue culture except from what I picked up from non specific tissue culture. Here goes! Well the last of the two experimental culturing fly traps contracted a contaminant. Due to my not sterilising of the sample, whoops. Also I have since read there are a whole other set of culturing mediums to be utilised. Time to ask a veteran for advice. Found a couple good reads one on the ethnobotanical page and sure to be more so back to the drawing board. Shame, I was looking forward to seeing what kind of growth would take place from a leaf. -
From the album: Experimental culturing. Fail.
I didn't have high hopes for this working but so far still green. Better than what I can say for my control which just had the MEA dextrose, indole acetic/ naphthalene acetic and GA3 . No iodine and no seaweed nutrient. But neither were dipped in bleach for contaminants or treated with ethanol. So I think it's just a matter of time until they die. -
From the album: Experimental culturing. Fail.
My control experimental plant culture has contamination. And on the other side of this picture there is Black Death starting at the base. But the one I experimented with iodine as an antibacterial is fine. I also read a bit up on it and apparently I should have dipped the plant leaf in dilute bleach then purified water. Then, oh,I can't remember off hand but I think it was a 70% ethanol dip, may have been just on the cut end for the alcohol and using African violet as the nutrient. I was tired when I read it so don't use this as an instruction by any means its just a pointer from what I remember. The other one appears healthy though. -
From the album: Experimental culturing. Fail.
#2. Culture Mixture Mixture 2. Consistent of MEA (malt extract agar) plus small portion of Dextrose (glucose). With main active ingredient indole acetic- naphtalacetic acid + GA3 (Gibberellic acid) although I don't know if it will work as there is no other nutrient other than the Malt extract and the Dextrose!! For essential growth it's necessary for NPK & trace elements but seeing I'm only looking for a significant amount of growth that it can be transferred into a pot. So fingers crossed, I think it's got a good chance at working, or not! One or the other are definite outcomes. ;) -
CheapÅ Glove Box for plant tissue culture.
enohpi-zeekim posted a gallery image in Member's Gallery
From the album: Experimental culturing. Fail.
One tub, 1 tube silicon, 1 pair elbow length chem gloves, 1 knife ( to go on the gas stove & heat up & melt the pre marked holes It's a Budget Box, don't knock it, "it's cheep"! -
From the album: Experimental culturing. Fail.
Venus Fly Trap leaf and 2mm wedge of plant tissue on MEA, dextrose, GA3 & indole acetic naphthal-acetic acid and seaweed liquid nutrient for plants...... Diluted with 1/2 ml 80% ethanol to dissolve the hormones into deionized water. Boiled and sterilised in glass jar with micropore tape over hole for filtered air exchange. One fifth of a mm grain of iodine crystal added to ethanol. Not enough to register at .00gram, so a pinhead size, added for extra sterility and to help dissolve the partially insoluble growth hormone. Then added to the water at 100ppm, mixed with the agar nutrient mix and boiled After pouring the agar nutrient growth mix the lid was lightly screwed on and jar placed into an oven bag. Placed in oven for 17 mins until starting to simmer then let to cool before any caramelisation of the sugars took place. Next day the oven bag with jar inside was placed in the glove box treated with bleach - 1part 4 - parts water! Let to sit & sterilise box. Plant cutting was taken with disposable scalpel and placed in steril beaker. Area sprayed down with alcohol around lid of Glove box, in surrounding air then inside the box. Finally the cutting (Venus F Trap) was placed inside and once closed and alcohol evaporated, the cutting was cut again fresh in the sterile conditions, finally wedged into the nutrient culture! The small wedge from the extra cut was left to make its own way in the medium,(in other words it was left to float around wherever it decides to settle, hopefully it takes root as its the smaller wedge that will be the interesting result. It may just die because it was exposed to air outside when cut)! Does anyone think this will work as a way to culture plant tissue? It's just an experimental mixture so if it works awesome, if not I'll have to read up on "correct" plant tissue culture, instead of using instinct (or my educated guess work). It's only cloning using much smaller samples in my opinion, I started with larger parts to begin with but will go smaller if I have any luck. Comments welcomed on this technique. Well been thinking on my method, think I should've tried hydrogen peroxide as a anti bacterial agent! Too bad I had none. But perhaps none would've been better than the iodine, I think it might have been the wrong thing to use for stopping competing bacteria or moulds. Another mistake may have been to try keeping it under a 30W light at night, at least at the stage it's at it's probably shocked and burned so I'm still waiting to see if any signs of death occur. Has anyone here got any plant tissue culture experience? I'm real interested if I'm far off, better google it! -
P. subaeruginosa print giveaway........ Microscopy use only.
enohpi-zeekim replied to spooge's topic in Mycology
Thanks I received mine already. Even got here before the strain I paid for. Good fella. -
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Yes I found that out now. Stupid law. A bit off research topic but still same fungi, I actually heard back in NZ that if people who wanted to eat them,( it was illegal of coarse because it meant picking and holding which would be harvesting and possession). But I heard,( & of coarse it's hard to know for sure), but, if you were to get down and pick them with your mouth it wasn't illegal if you ate them because its called grazing! Lol! Yeah human grazing rights. So if you have the right to graze on grass and herbs and just happened to eat psylicibe mushrooms it was legal. Anyone know if that's true? Yeah this cool freaky hippy dude from west coast of NZ told me when I was doing a South Island tour in a Winnebago.
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Help Identify this plant!
Images added to a gallery album owned by enohpi-zeekim in Member's Gallery
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From the album: Help Identify this plant!
I thought I had this plants identity sorted but I seem to have misidentified it. Anyone have a fix on what it is? It has a small yellow flower about 2-3mm size, a lot like a fern in the way it branches from the soil with a brown fibrous root and small nodes forming just beneath the ground before sending up another branch. I had thought it was a ephedra but it doesn't have the same jointed stems as E.sinica perhaps it's another variety? Yellowish to dark green colour, branchy twig like structure. Would love to find out if I am actually right to it being e.sinica but a a seedling next to it has definite differences, the way it doesn't have a full jointed structure. -
P. subaeruginosa print giveaway........ Microscopy use only.
enohpi-zeekim replied to spooge's topic in Mycology
Very generous of you. I won't say no, in fact I'll say thank you very much sir/madam. ( [email protected] ) -
Tek: Soma_seeker's home-made laminar flow hood for $180 (or less...)
enohpi-zeekim replied to soma_seeker's topic in Mycology
About the recent problems with bags, have you been using the same stock of bags? Maybe the bags are contaminated from storage? Just the first thing that came to mind when I read it. Perhaps I don't understand the use of bags fully yet. Good work on the flow hood, nice inspiration . -
Tek: Soma_seeker's home-made laminar flow hood for $180 (or less...)
enohpi-zeekim replied to soma_seeker's topic in Mycology
It's cool, I thought flow hood had a hood type thing like you'd use in a chemistry reaction to avoid gas build up. Nice to learn that these can be referred to as a laminar flow hood also. I've seen one in action but didn't realise it was just one of these. Good work. -
Unreal, I didn't know that they could fruit only on PDA. Well I would have to imagine that answers my Q. If the culture is able to fruit and the mycelium bruises blue then it must be active with psilicybin well before they fruit. Cheers for that.
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Does anyone know, is it illegal to grow mycelium alone? I just have been acquiring spore prints to look at the spore differences and I wanted to culture mycelium on Petri dishes to see how the different mycelia goes through growth stages. Is it illegal to even grow a psilocybin species to this stage. Obviously they would never be able to reach a fruiting stage on a culture dish and so there would never be a mushroom, I am not familiar with Australian law regarding this matter yet. Anyone know where I can find the answer in legal form, not just by, say so. I know it is illegal to grow mycelia with the intent if growing mushrooms but for there to be intent there would need to be more than Petri dishes with a growth pattern in? I would have believed it would need a humidity dome or a substrate with potential to grow fruiting bodies on? As agar lacks potential to grow fruiting bodies from my knowledge, I had thought it possible to grow the mycelium for research without licience. Please can anyone clarify this and provide a link?