sappyroot

That is incorrect. The reason for the bleach and rinsed with Hydrogen peroxide is the sterilise the seeds. They are then placed in-vitro which is usually a MS solution that needs to keep in aseptic conditions. Sadly bacteria thrives in the media used for Plant Tissue Culture. If you are interested in this area there are many books on Plant tissue culture. Most require little biology background. Very accessible to most. also, I have some number of published papers on PTC and Cactaceae. Dylan.

Hello all, I've recently taken to putting down a large volume of seeds. As it stands i've got over 50 containers with 100+ seeds per container germinating, with a variety of species of Cactaceae. I've also got over 150 containers with 6/8 cuttings per container of Hylocereus undatus, which I purchased from an aussie dragon fruit grower for use a rootstock. I had tried to purchase the following seeds but alas problems occurred. L. koehresii, L.jourdaniana, L.fricii, L.alberto-vojtechii, L.diffusa, L.Mischung, L.williamsii. I've been collecting succ/cacti for some years now and decided i would like to move my collection with the long term goal of developing and selling plants/seeds.I've only just started this transition. Can anyone here help with with the following seeds, in say batches of 100? Thanks Dylan

To be honest i'm not experienced enough with these seedlings to say. I put them down in a container culture about six months ago. All the others came up green. This one came up red and stayed that way. I think i'll graft it and see what happens.

Seeds I recently purchased and put down ( Last couple of days) each one x100 seeds. Ariocarpus #26-fissuratus /16/ rough tubercles, purple flowers #31.8-kotschoubeyanus v macdowellii /17/ pink fl, tiny star stem #36.9-retusus 'furfuraceus' small densely packed tubercles #24.95-agavoides /14/ deeply seeted stem, slender tubercles #40-trigonus /14/ long grey tubercles, yellow flower roseiflora 6.4-roseiflora /14/ short yellow spines, super pink fl Astrophytum #48-asterias 'Superkabuto', densely covered with white woo 62-myriostigma v nudum /15/ smooth pure green stem 70.64-X MYR-OR hybrid, myriostigma x ornatum 70.1-capricorne -myriostigma-ornatum-senile, all hybrid mix, weirdos #47-asterias /16/ small smooth green stem, shiny fl Aztekium 72.396-hintonii LH442 El Rio, NL, wooly areoles in youth Blossfeldia 74-liliputana stem with soft white tufts of wool 74.36-subterranea tuberous roots in rock cracks Cephalocereus 76.36-senilis /14/ tall stem covered with long white hairs Cleistocactus 82-strausii many white spines, long red flowers Copiapoa 85.89-tenuissima thick soft almost black stem, wooly crown Echinofossulocactus 298.49-dichroacanthus N83.022 Guanajuato/San Luis Potosi border 299.14-phyllacanthus 'grandicornus' SB437 /16/ Huizache, long sp Epithelantha 353-micromeris /17/ tight white spines, tiny button-stem Escobaria #397-sneedii SB173 /18/ Dona Ana Co, NM, mostly pink flowers Espostoa 4.77-lanata slender branching white wooly stem 416.608-nana short stem, super white wooly Frailea 434.11-asterioides Arapey, Salto, flat shiny reddish stem Leuchtenbergia, /3,6,8,13,16/ long tubercles, deep taproot 522-principis /17/ long thin papery spines, deep taproot Neoporteria 1033.26-napina ssp lembckei KK113 Maitencillo 500m, tiny spines Obregonia, /3,6,13,14/ flat stem from Mexico #1086.3-denegrii green incurved tubercles, white flowers Ortegocactus, /3,6,8,14/ 1123.294-macdougallii icy blue stem, rusts in age Strombocactus #1246.6-disciformis Xichu, Gto, 'pulcherrimus' dark red fls Turbinicarpus #1292-polaskii tiny stem with broad fat tubercles #1290.5-lophophoroides Buena Vista MESEMB Aloinopsis 1316.6-schooneesii DT4869 Springbokvlakte, pebbled leaflets 1311.5-luckhoffii e Kamas, rough concrete chunks 1313-malherbei /17/ fan-shaped warted leaves, orange fl Argyroderma, /C,15/ thick leaves, flowers when small 1320-congregatum HH5032 near Vredendal, elegant eggs 346.27-mix smooth rock-like plants, wonderful pastel flowers Bijlia 1352.1-cana CM32 Prince Albert, delightfully unequal Conophytum 1435.3-obcordellum 'declinatum' BM7928 Botterkloof, spidery fl 1473.4-spp olla podrida, full mix of species Lithops 1570.9-dorotheae F357 large open red-orange window 1596.9-hallii Klippunt, Upington, wonderful bluish reticulate form 1616.08-hookeri C110 deep dark red jagged lines 1774.3-mix stone plants, colorful faces, variable colors + patterns Seeds I just purchased and will be putting down in the next couple of days... LOPHOPHORA koehresii 10K, Encarnacion 100 Seeds LOPHOPHORA jourdaniana 10K 10 seeds LOPHOPHORA fricii 10K,El Amparo - v La Pa 10K 100 Seeds LOPHOPHORA alberto-vojtechii 10K,SLP 10 seeds LOPHOPHORA diffusa 10K 100 Seeds LOPHOPHORA Mischung 100 Seeds LOPHOPHORA williamsii 10K 100 seeds

Hey Guys, I've been putting down a lot of seed lately. About six months ago i put down some Ariocarpus fissuratus to my surprise one of them is totally red. I've yet to attempt to graft it, but it seems to be growing fine, all new growth is red too. No signs of green. I do have some Pereskiopsis spathulata ready if need be, what do you guys think? graft of leave alone?

Hey all, I dunno if this link has been posted before but its definately worth a look. http://public.fotki.com/PanosS/

Hey All, I've just put down some varieties of ariocarpus and lophs seeds. I'm looking for some Pereskiopsis cuttings to graft onto. Anyone got any to spare? *Edit* oh yeah forgot to mention I'm willing to pay. Thanks Dylan

NP's I was trying to source somewhere in AU to get MS and growth hormones but I can't find anywhere that will sell to individuals. There was one place however that does international orders http://www.hometissueculture.org/catalogHTCGsupplies.htm If anyone knows of somewhere locally please let me know. EDIT* just found these youtube videos for anyone wanting to get started http://www.youtube.com/watch?v=Oh8804Kfdps...feature=related I'm planning to do some in vitro cultivation of lophs, aricarps, and some grafts onto peresks too compare the growth rate, pups development. I'll keep you guys informed if interested. P.S to the member here who said he would send me some peresks cuttings, I'm still keenly waiting

Hello all, After doing some googling on "areole grafting" and I came across this post on the nook. "Inducing pupping, with different chemicals" Seemed interesting. after doing a little more googling it seems a lot of these chems are used in tissue culture. So i thought I would post these links here if anyone was interested in giving it ago. http://www.gwf.org.bd/IJSCP/Fast%20Track%2...%202007/1-8.pdf http://www.springerlink.com/content/00x103...u8/fulltext.pdf and a homebrew tissue culture setup. http://www.biotechnologyonline.gov.au/pdf/...re_in_class.pdf

I'm after some Pereskiopsis for my first attempts at grafting. You wouldn't happen to still have any spare?

Ok, thanks for the replies ,everyone!.

Here is a picture of it side by side with one of my other C.peruvianus Monstrose.. The new one has around 7-12 spines per section. The spines are orange color and about half a centimeter longer then the bigger one which has black spines of 3 per section

Hello all, I picked this fella up earlier today, If the pictures arn't good enough I can take some better ones. Thankyou.

Well I can't give you the seeds, Sadly the plant isn't mine. But I can get in contact with the people who run the show and ask if they could give me some form of contact for the person who owns that Loph. Sapp.

I couldn't tell you, I didn't talk to the owner of that beauty.