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The Corroboree


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Posts posted by Darklight

  1. Dropped in and saw the start of your talk, but didn't stay cos the tent was chockers and we were getting rained on.


    Missed a chance to pour over your book with you, was wanting to show my colleague your work, we'll try again next year ( don't go into town much, us all out here )


    Geez you had a good crowd tho, were you happy with how things went?


    MG this year was interesting, there were a lot more people in the town hall for the speakers this year and an org I spoke to said many had actually been there all day and were taking notes


    I loved the increase in diversity of the stalls, the demographic seemed slightly older this year ( hard to say tho ) and Saturday seemed nice and chill. Crowds seemed down for COVID uncertainty and the rain.


    Cops, as always, heavy handed at the start on the RDTs ( someone said on FB that by Sun some lighten up a little and smile occasionally ) but there were plenty of deso drivers and commercial lifts into town for some pretty reasonable rates.


    Hope you had fun as well as a great talk



    • Like 3

  2. On 16/02/2021 at 7:48 AM, Buttsack said:

    Wanna crawl any further up his arse? lol


    It would be most incongruous if I *didn't* reply to your stupidity. I've known the bloke years longer than he's had the forums. You probably don't understand what it's like to have long term friendships; in that event try to extrapolate possible scenarios via google


    On 16/02/2021 at 7:48 AM, Buttsack said:

    "Torsten's forum"
    Wanna elaborate on that?


    He owns the domain. For most of the decades it's been around he's paid for and managed the site. Occasionally a few people kick in funds, some most generously


    Is this simple enough for you?



    On 16/02/2021 at 7:48 AM, Buttsack said:

    "start your own pages and forums"
    I don't think you understand how forums work, it's not the owner who makes them what they are, it's the users, and if the owner kicks out users willy nilly people do find other places or make them.


    Which explains the unmitigated proliferation and long term survival of the hundreds of other ethnobotanical forums online today /sarcasm





    • Haha 1

  3. 7 hours ago, trucha said:


    A preprint of the version that did go to press is available at https://cactusconservation.org/resources/cci-publications/



    Trucha, that index is a veritable smorgasbord of articles, not just the one you quoted above :worship:


    Last I saw you speak at EGA outdoors some years back you seemed to be having a fair bit of a hard time keeping your work going?


    Now I see the output from your group, with your name on so many peer reviewed publications *and* open source. Am utterly impressed by your resilience, attention to detail and dedication. Thank you so much.

    • Like 2

  4. And yet Buttsack, here you are, on Torsten's forum, spitting the dummy after having a dummy spit at him on the FB page he runs as well. And you're not banned here so you get to blather some more. He's been very patient with you IMO


    If your SAB related existence is as bad as you say, fuck off, start your own pages and forums. When you've done this for a couple of decades and dealt with all the issues you will possibly be taken more seriously

  5. Sounds good


    If you are starting from spores though, remember you can't add hydrogen peroxide to your media. Hydrogen peroxide kills spores


  6. 2 minutes ago, john said:

    Many thanks for the clear response. I just prepared some agar from a health food shop boiled with water - nothing else.

    I will try H2O2 as suggested in the mix next go. Mine have started to grow but are moving very slowly after 2 weeks. Maybe I 'm

    used to rapid growth in the tropics.


    K- so you have made what's called water agar


    It's not really nutritious at all and any growth you get could be resulting from residual nutrients in whatever it was you inoculated it with. That could be why growth is slow


    A general purpose agar is something like Malt Extract agar


    There are a million recipes for Malt Extract agar, often just varying the amounts. One that's worked for me for a variety of species is:


    20g/L light malt extract ( brewing supply shops sell it )

    100mg/L garden lime ( Bunnings )

    100mg/L potassium dihydrogen orthophosphate ( some people omit this )

    20g/L agar ( health food shops )


    A litre of agar is heaps! Like, about 40 standard petri dishes worth. So work out how much you actually need and change amounts accordingly


    If you want to add 3% hydrogen peroxide at 2-8ml/L, do that after you have cooked your agar and the container is at a temperature which can be held comfortably in your hand


    Malt Extract Agar is really nutritious. Lots of species like it- so do lots of contaminants.


    Not all species are happy with you adding hydrogen peroxide, but many will overcome their distaste for it and grow after a couple of weeks


    Good luck!

    • Like 2

  7. 13 hours ago, john said:

    I am starting out with shroom growing. I see a lot of recipes for
    agar. I have mycelium  growing in straight agar. Should I use a mix?

    When peroxide is used is that just for sterilizing or is it included in the agar mix. 

    sorry to be so ignorant.

    john S / Nimshie29



    Hi john


    Welcome to the wonderful, frustrating, fun world of growing mushrooms :)


    The agar question is a helluva lot to unpack. There are a bazillion recipes for 'agar' ( which is really only a generic word for whatever gelling agent is used to solidify the semisolid stuff in a petri dish, but the common use now includes whatever else is in the mix ). Confusing as fuck IMO


    Most people just use what's convenient for them that works, hence all the different agar types. Some types are specific to species, some are general, some used to deter competitors to get a cleaner culture etc


    Are you using only agar and water- your OP refers to straight agar- or is there other stuff in there, malt extract, dog food, vegemite, lime etc


    H2O2 ( hydrogen peroxide ) kills spore of bacteria and fungi without killing most mycelia- up to a point ( it can be overloaded, degrades if you keep the agar plates for too long, and shouldn't be added until your agar has cooled to the point where you can hold the container by hand )


    3% hydrogen peroxide is usually added 2-8ml/L when your agar has cooled and is ready for pouring. It can negate the requirement for pressure cooking your agar and is also handy if you don't have a sterile cabinet or still air box to work in. I've poured H2O2 agar in the open in my kitchen and had good success with it. I usually carry some plates of it with me if I'm taking samples from mushrooms I find on bushwalks


    You don't need H2O2 added to agar, but there are some situations which it really helps. Some species don't like it much and some take a while to establish on it


    Hope that clears things up. Good luck!


    • Like 1
    • Thanks 2

  8. On 03/11/2020 at 5:25 PM, prier said:

     My journey into plants basically started here so I figured this is a good place to announce I now run my own nursery.



    Hey Prier- that's awesome news, congratulations!


    Was really looking forward to seeing you at EGA this year, I've been coveting some of your lovely cacti offerings there for a long time and a catchup with you is always lovely


    Took me a minute to find your ebay store, but there you are:




    You'll rock it! Coupla decades experience in cactii will serve you well :worship:






  9. On 08/08/2020 at 8:46 AM, sagiXsagi said:

    my father has started to seem hesitant to go to swim, he seems to be having a harder time to navigate and move his limpbs in water... 


    Sorry to hear about your dad mate, but it's beautiful you think of him so highly


    My dad's had Parkinsons for a long while, as have a few others I know, I've been following the research and their progress for a while. Unfortunately none of the people I know with Parkinsons are interested in support outside their direct relationship with their GPs ( who mostly don't know much about Parkinsons )


    But swimming's one of the best things your dad can do, if he's having trouble can you get him to wear a flotation device? I know there can be a bit of pride about wearing one in a public place... but swimming's so good for stages of PD. In Australia I've seen water exercise used as a recognised form of therapy for PD and it did work for my dad as a part of slowing his Parkinsons progression





    I might seem like an idiot to be asking 


    No, you seem awesome and loving. I wondered about those compounds too back when Dad was diagnosed. I think you're right about potential deficits after use. Am not a physician and there are people here who are better qualified than I to explain the mechanisms


    There are some biological compounds I'd look at first before directly going for the pure stimulants


    Mucuna pruriens seed could be a good place to start if he's not already on meds ( buy it in, or only grow the hairless subtype )


    CBD is approved in Australia for treatment of some PD symptoms. Not sure about THC, but CBD is definitely on the list and I've heard ( and only heard- not seen yet ) good things of it for this. Not sure about legality/ accessibility where you live. Is most likely to be well tolerated alongside other medications


    Ibogaine root bark has been reported to reduce symptoms of PD too. There's a bunch of glowing reports on that if you google ( I do not trust glowing reports on Google much, but low dose ibo is awesome and I'd personally give it a go if I ever got a diagnosis) - but if you're recommending it to anyone else- particularly if they're already on other medication- I'd take real care to find a sympathetic primary health care professional and a good clean source of ibogaine root bark


    Was most interested to hear psilocybin/ mushrooms mentioned above, now I'll have to do some reading :)


    All the best for your and your family's journey.

    • Like 4

  10. 21 hours ago, lindsay said:

    recipe- 1 gram of chelated iron per 100 grams of dry grains.

    mixed into the cooked grain before sterilization.


    Whoa- lovely work, seriously impressive. Definitely warrants further investigation at other sites

  11. I've got an old Olympus ECEBi microscope and I love the damn thing. However it's a little dusty inside, and I'd like to do some spore identification with it, so I need crisp vision at high magnification


    A few mates have the same base model or a variant thereof. They were super common back in the day


    Yep, they're from the 70s or something, but they're well made. I love well made things :)


    Having trouble finding someone who will clean it- not just the outer, but the gubbins inside, which seems dusty


    Have spoken to a few professionals about it who have said it's a fair bet they're not worth looking at, and the average hourly rate ( including part thereof ) for a professional clean is about $200 an hour- that would also cover the evaluation, even if no work was done


    Bit lost with what to do about this. I'd also like professional advice on storing the damn thing so it doesn't suffer from fungus, which seems to  get into a lot of scopes in NNSW


    Anyone have actual experience with getting this done? Not just links- but actual experience?

  12. Yep, I wouldn't throw fungicide or glyphosate on it. Ick.


    Have a crack at throwing a fair whack of Aglime on it. Raising the soil pH might discourage that species and encourage a different one. Whether it's one you like or not is another matter


    If you have heaps of local used oyster mushroom straw  or other local spent mushroom spawn I'd throw that on top of the aglime.


    I haven't tried this, it's just something that's inline with basic mycological and biological processes. Inhibit the thing you don't want, encourage the thing you do


    And saguaro is right, there will be spores aplenty, and mushrooms will always come up when conditions are right for them and not their competitors


    Yellow stainers prolly occupy an important niche, don't wipe em out


    Let us know how you go



    • Like 2

  13. 17 hours ago, Tripyamine said:

    Hmmmmm I have a nice starter if anyone wants it le PM me 


    I really like bags to grow in for a small scale, it's very popular in the industry too. 

    If you get a bad infection/contamination you can burn your bags and reroll otherwise you can just keep reusing them.

    We use Polypropylene bag rolls and seal them ourselves to the size we need, and then sterilize with 70% ethanol and UV


    It's a good rule of thumb to always do a 1:5 inoculation with any algae (1:10 if you can't help it) 


    I'll grow up a 5mll culture from a stock or a plate, scale that up to 25ml, then to 100ml, then 500ml, then 2.5lt and then start a bag culture with 10L and gradually increase the volume to 20L.

    Then its too a big 100-200L bag like the one pictured and then to ponds/pools all the way up to 400kL



    arthrospira (spirulina) likes to grow under basic conditions up to like a pH of 10 so lots of media reflect this as its useful for keeping lots of the contam/bugs out.

    Some good info on making the standard media here but you might struggle with some of the chems, also good culture info too. 

    http://sagdb.uni-goettingen.de/culture_media/02 Spirulina Medium.pdf


    Another good media to use that you can purchase heaps of for cheap as is Cell-Hi JW (should be around $40 for a kg that will make close to 1000L), you will have to google around to find a supplier as we make our own and haven't had to buy for a while. 


    At the end of the day if you scale up slowly and use appropriate media it's pretty easy to do, Spira harvests very easy compared to some other alga, but chitosan always makes it better! 

    Sparge with lots of air and give em plenty of light (outdoors is better than LED but cheap eBay grow LEDs will work fine just gets expensive >100L)





    Wow. Just wow. Awesome, thank you so much! I knew nothing of these.


    What's the yield like for a domestic setup? Either per kg or how many single portion meals or meal additions you'd get per bag?


    This is now one of my one-day-I'll-get-to-it projects. Glad you got there first :D

  14. That. Was. Beautiful.


    Thanks to Ronny, Obtuse and Adele and the EGA crew for putting this together. Was very much worth the trip to Melbs.


    Panel was gorgeous, hell, the audience was gorgeous. A lovely mixed bunch of age and backgrounds, some were familiar with EGA's work, others knew more about the myco-side but quite a few knew of both.


    Audience had a pretty good grounding in all things mushroomy, which made for some excellent questions to the panel. The book table near the ticket point was often crowded with people who had at least one of the references if not more before they even got there. The venue was awesome


    The panel. I am such a myco-groupie. They all made themselves available for chats before the event and during intermission and a few stuck round afterwards and they were all fucken lovely people with a grace I can only envy. Question time was professional, comprehensive and respectful and thus also awesome


    If you get a chance, go to one of Dr Alison Pouliot's workshops or forays. She's got a really impressive schedule the next few months, quite a few covering specific species of interest ( how cool's that ) and apparently they book up full- fast. https://alisonpouliot.com/workshops-events/


    Sapphire and Fungi4Land: https://fungi4land.com/ about the most worthy book project of the decade. Love her work. I will buy all the t-shirts.


    Fungimap and iNaturalist. Best citizen science work on the entire planet ( I will find you that reference Cam, I promise ) Do they even need a link? Everyone knows em. Here, have the link anyhow https://www.inaturalist.org/projects/fungimap-australia


    Ah yeah, the film. Didn't get to see it. Sure it was fantastic tho, everyone seemed to like it :D


    Kudos to you all the lot of you. And thanks again


    Bonus points to me for being lucky enough to hang out with Obtuse heaps, and for working out how to use the bloody coffee machine thing at the flat

    • Like 2

  15. On 30/12/2019 at 4:14 PM, Calaverite said:

    Darklight how difficult is the tissue culture process?


    From seed? Easy. A great beginner's project, really worth a go.


    Sow a lot, some in individual containers, some with a few per container.


    Split the batch in half and put one of the halves in the fridge for 3-4 weeks then return it to your light rack.


    If you wanted to start from a live plant it could be more challenging. Start with seed in tissue culture and let us know how you go

  16. On 08/01/2020 at 9:21 PM, RonnySimulacrum said:


    The screening will be followed by a mycological panel featuring ecologist and environmental photographer Dr Alison Pouliot, mycologist Caine Barlow, ecologist Sapphire McMullan-Fisher and President of PRISM, Vice President of EGA, research fellow in Medicinal Chemistry at Monash University, and co-lead investigator of the upcoming Melbourne psilocybin trials at St Vincent's Hospital, Dr Martin Williams. The mycological panel will be facilitated by Cam Durnsford the coordinator of Fungimap.



    Ermahgord. I just saw this.


    I am very seriously thinking about heading down to Melbs for the night on the off chance I get to even be in the same room as the panel crew, all of whom are my idols

  17. 12 hours ago, Calaverite said:

    Based in Melbourne I am chasing some quality wasabi variety either division or seed.



    i sent some down to ReShroomed a few years ago ( mmm, maybe a decade now I think about it ). TC clones of seed grown. They grew down at Colac like corkers and the leaf size was 6x what I got in NNSW within weeks


    ReShroomed passed away a few years ago :( No chance of getting plants out of him now either...


    allrareherbs.com.au site says they're delivering Wasabia offsets in April


    But try the seed too. Even if you don't end up with a commercial-grade wasabi root, the leaves are great for winter salads and the stalks apparently are useful too


    Give it a go I reckon