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In Vitro Acacia acuminata


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#1 Monk

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Posted 09 March 2011 - 05:03 PM

Yes, I'm cross posting, but I just haven't posted here in a long while, miss you guys and thought you might like to see some work with one of your natives :)

48 hours after deposit onto agar media (!):

Attached File  12111 005.JPG   96.55KB   10 downloads

Some days later:

Attached File  13111 001.JPG   84.27KB   9 downloads Attached File  13111 004.JPG   82.77KB   25 downloads

10ish days after that:

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Around the same time as the above picture, various parts of a seeding were placed on callus induction agar....days later, it was clear that the hypocotyl was rapidly producing callus tissue. Pardon the lack of focus in the following pic. The camera was fuckin with me:

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That turned out to be the best portion of the seedling from which to produce callus and here we have it's progress one month later:

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That black dust is just rust from the old ass tyvek-mason jar lids I'm using, but everything is good and sterile :) Stay tuned for liquid Acacia culture ;)

Oh, and I've also put some of the other seedlings into shoot multiplication media and will post pics when they make more progress. Next up'll be phleb. I've a few seeds and some tricks up my sleeve with this plant TC work to hopefully achieve success.
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#2 Mycot

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Posted 09 March 2011 - 05:17 PM

Very very cool. :worship: I'll be keeping an eye on this thread.

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#3 GoOnThen

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Posted 09 March 2011 - 05:55 PM

Great stuff Monk :worship:

I have also been watching your other work with tissue cultures and have been enjoying the learning experience. I just have to do more reading to understand it more.
I have to find myself some good books probably tissue cultures for dummies would be a good start :)

Cheers
Got

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#4 Darklight

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Posted 10 March 2011 - 07:29 AM

Congratulations! Seriously beautiful :)
All the theories in the world won't help if you don't run an experiment. Just do it. And take good notes

#5 tripsis

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Posted 10 March 2011 - 08:30 PM

Great stuff Monk! :)
Since we depend on an abundance of functioning ecosystems to cleanse our water, enrich our soil and manufacture the very air we breathe, biodiversity is clearly not an inheritance to be discarded carelessly. Edward O. Wilson 1992

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#6 Eluna

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Posted 12 March 2011 - 01:51 AM

i likey! keep it up!! and keep us informed :)

#7 Darklight

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Posted 29 June 2011 - 02:48 PM

So... what happened- any progress? It was looking good back in March
All the theories in the world won't help if you don't run an experiment. Just do it. And take good notes

#8 mutant

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Posted 14 March 2012 - 11:53 AM

Awesome work
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#9 Darklight

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Posted 08 April 2013 - 12:45 PM

Any updates on this? Would be good to see someone working on it. Hope you've made progress :)


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#10 migraineur

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Posted 08 April 2013 - 05:47 PM

Great stuff Monk :worship:

I have also been watching your other work with tissue cultures and have been enjoying the learning experience. I just have to do more reading to understand it more.
I have to find myself some good books probably tissue cultures for dummies would be a good start smile.gif

Cheers
Got

 

I've got at least one good ebook I could hook you up with. I have a collection of gardening ebooks etc.