IceCube Posted April 19, 2015 just go dig up soil from around other wattles to innoculate. rhizobium is everywhere. and probably better to use from soil as there would be a better compliment of associated bacteria and fungi. Has there been success with this? As I am doing tissue culture experiments, I would like to obtain a pure culture (but then I should just buy it lol). One experiment I was going to try was making a homogenate of some acacia nodules and culturing them/innoculating directly. Share this post Link to post Share on other sites
obtuse Posted April 19, 2015 ah i see your point. yes i have had success with my method. but if you want a pure culture, the rhizobium within the nodule are maintained within a sterile environment, therefore would be easy enough to extract and then culture. go find some longifolia dig up some seedlings and you should have plenty of nodules to work with. my understanding is that rhizobia are only specific to genus not to species, but given how big the acacia genus is, findign a species closely related should mean that the rhizobium should be fine. 3 Share this post Link to post Share on other sites
theuserformallyknownasd00d Posted April 19, 2015 Flori's make a great hedge plant! These ones are 5mths in the round, went in as 3" single stem tubes 6 Share this post Link to post Share on other sites
Tripyamine Posted April 19, 2015 ah i see your point. yes i have had success with my method. but if you want a pure culture, the rhizobium within the nodule are maintained within a sterile environment, therefore would be easy enough to extract and then culture. go find some longifolia dig up some seedlings and you should have plenty of nodules to work with. my understanding is that rhizobia are only specific to genus not to species, but given how big the acacia genus is, findign a species closely related should mean that the rhizobium should be fine. Good thinking! escaped me about the sterility Share this post Link to post Share on other sites
incognito Posted April 20, 2015 (edited) Yea well upon thinking upon my hedge I'll probably that the 9 or so accuminatas out and replace with phleb plants if the phlebs keep steaming along as they are. Winter will be their big test. The fresh gorgeous red growth on phlebs will look amazing on a hedge. I can always remember my old rage teacher banging on about how acacias make awesome hedges. I'm basing this hedge entirely on his recommendation if it fails it fails of successful will be fricken awesome and a way to perhaps get these plants working in landscaping. Those floribundas would hedge well Edited April 20, 2015 by incognito 1 Share this post Link to post Share on other sites
mutant Posted April 21, 2015 epic thread should re-discover soon! Share this post Link to post Share on other sites
upside Posted May 1, 2015 Here's 5 of 7 that I have on the go. I got experimental with a couple, after they didn't germ so I removed the seed coat with my finger nail, it was quite easy after being damp for a few weeks. It just slipped off lol Put back in the tube they popped up not long after that. The others I left and came up. Pretty tuff lil dude so far. 7 Share this post Link to post Share on other sites
Grundle Sailor Posted May 7, 2015 Fuck yeah cunts, greatz 2 c sum shit hot work on these hard cuntz, mine is groin ok , fast but steady Share this post Link to post Share on other sites
theuserformallyknownasd00d Posted May 7, 2015 Bullit? Is that you buddy? ;) haha sorry real bullit and I feel obliged to leave on a note of fuck yeh cuntZ 2 Share this post Link to post Share on other sites
Amazonian Posted May 8, 2015 ^ lol, missed this post. Was already thinkng same thing. Lol. 1 Share this post Link to post Share on other sites
Grundle Sailor Posted May 9, 2015 Think I no one cunt here and he's a cunt but it ain't bullet or purple helmet so no, what's a fuck u on bout Share this post Link to post Share on other sites
Sallubrious Posted May 9, 2015 (edited) Edit Fair enough OB, I'll take it all back. Edited May 9, 2015 by Sally 2 Share this post Link to post Share on other sites
obtuse Posted May 9, 2015 (edited) why derail this awesome thread both of you ? Edited May 9, 2015 by obtuse 3 Share this post Link to post Share on other sites
Grundle Sailor Posted May 10, 2015 Fucking awesome thread thx cuntz. How r Yours going obtuse? Share this post Link to post Share on other sites
obtuse Posted May 10, 2015 Going well. getting cold and wet so they will be really happy. just like their natural habitat sans snow. 1 Share this post Link to post Share on other sites
shroomau5 Posted May 10, 2015 Do you have any phlebs Grundle sailor? Share this post Link to post Share on other sites
Grundle Sailor Posted May 11, 2015 Haha yeah mate for one thatz doing ok. Love em. Grown from seed to its no easy thing. You? 1 Share this post Link to post Share on other sites
shroomau5 Posted May 11, 2015 Sure I have one. I love them too. I didnt grow it from seed though.. It is definitely loving the cooler change in the air. Share this post Link to post Share on other sites
Grundle Sailor Posted May 12, 2015 Cuntz don like humidity here in bris, fuckjn comin back to live now! Share this post Link to post Share on other sites
obtuse Posted May 12, 2015 these are cold loving trees. you can tell they dont like too much summer heat. some of the ones i know in vic dont like the heat and it shows. 6 Share this post Link to post Share on other sites
☽Ţ ҉ĥϋηϠ₡яღ☯ॐ€ðяئॐ♡Pϟiℓℴϟℴ Posted May 13, 2015 what excellent news ! 2 Share this post Link to post Share on other sites
Grundle Sailor Posted May 13, 2015 Mate I'm fuckng tempted to get the ice. Locks out and pack the cuntz around the roots ova summa!?! Share this post Link to post Share on other sites
obtuse Posted May 13, 2015 just keep em cool. theyll be fine. Share this post Link to post Share on other sites
shroomau5 Posted May 13, 2015 (edited) We had a brutal summer here. We were still getting 33c in April. 30c today. I can't ever remember it being this hot consistantly this late in the year. My phleb still grew and threw out new growth during summer. The leaves would turn leathery looking as i had it in full sun most of the day but it showed no real signs of distress. I would say its highly adaptive. I will treat her kinder this summer with a nice cool spot in a green house Edited May 13, 2015 by shroomau5 1 Share this post Link to post Share on other sites
obtuse Posted May 14, 2015 good to hear. sounds like a plan Share this post Link to post Share on other sites