printing store bought oysters?

[gnome82]

Hiya all,

So I have tried this several times now no luck,

According to Stamets pleurotus can produce half thier dry weight in spore!

Have attempted to print both coles and woolworths varieties with no luck,

This doesn't seem to be problem for agaricus

Was hoping someone could shed light on why they will not produce spore?

Id imagine this is commercially desirable

I do have two theories, 1- a non spore producing strain possibly gmo?

this does not make sense though as it is the purpose of mushroom fruitbody to produce spore

2-low temp storage ceases spore production?

Interestingly they do seem to revert vigorously back to growth mode mycelliating the paper they are supposed to be printing on! This has led to many experiments, but the rapid growth seems to stall after about a week

[Zen Peddler]

Clone them mate.

[obtuse]

I agree, clone them.

when you get to fruiting, then print while their young.

shop ones may have finished by the time you get them.

[ace1928]

I've cloned a blue oyster from the shops that was actually a sporeless variety. A lot of important commercial strains ARE sporeless to help avoid health issues with workers.

[gnome82]

Am trying to clone aswell, not sure how well it's going...

Think this strain might be more impatient than I am!

[ace1928]

Try wrapping some plastic around the dish to limit oxygen. Might stall the pins and stop it from trying to make more. Worth a shot at least

[Berengar]

Premature pinning can also be a sign of too nutritious agar mixture.

[gnome82]

Yes agreed need to try something,

the premature pinning must be using energy better put towards mycelial growth at this stage

will try couple layers of clingwrap hopefully enough

have read 10 micrometer garbage bags are permeable enough to allow gas exchange

Am using h2o2 in agar, will this be a source of excess oxygen?

the mycelium must need some o2 though

The agar mix was 12gm malt and 5gm sawdust 1gm yeast and powered milk per Litre

now I could be wrong but I thought this was on the lighter side of recommended nutrient,

just trying to eliminate possible causes

[worowa]

Yes agreed need to try something,

the premature pinning must be using energy better put towards mycelial growth at this stage

will try couple layers of clingwrap hopefully enough

have read 10 micrometer garbage bags are permeable enough to allow gas exchange

Am using h2o2 in agar, will this be a source of excess oxygen?

the mycelium must need some o2 though

The agar mix was 12gm malt and 5gm sawdust 1gm yeast and powered milk per Litre

now I could be wrong but I thought this was on the lighter side of recommended nutrient,

just trying to eliminate possible causes

Spores don't like h2o2...

[ace1928]

Spores don't like h2o2...

That is putting it nicely

[fungi1963]

H₂O₂ is only for clean up work or running mycelium of already germinated spores. You use it to prevent spores from germinating, hence good for high contam or low-sterile environments.

Good for wild cloning too where you can dip the harvested fragment of mycelium into H₂O₂ to clean up the cut surfaces prior to applying to agar plates.

[gnome82]

well there was just no stopping these pins

2 layers of clingwrap wasnt enough to slow growth, not havin a go ace, just reporting progress

fortunatley there was also mycelial gowth aswell as pinning, which on first transfer appears to be continuing to grow without pinning

now I could be wrong but I thought this was on the lighter side of recommended nutrient,
just trying to eliminate possible causes

appologies if this came across as pretentious, my comunication skills are poor at the best of times

there probably should have been a question mark in there or something as i was trying to check wether the agar mix was too nutritious or not

anywayz, am loving H202 agar, no more f*#kn glooveboxes!

for the first time i do not resent the awquardness of agar work,

but obviously this will make starting from spore somewhat challenging