Darklight Posted May 1, 2011 Aseptic germination of Iboga is really difficult and relies on excellent sterile technique and catching the seed at the right stage of development. The latter is almost as difficult as the former. Given that the seed coat probably contains tannins and f*k knows what else, and in vivo relies on a few cycles of wet/dry prior to germination, is there some chemical process beyond simple water leaching that could be inferred and easily replicated? Can anyone estimate what might happen to compounds remaining after the initial leaching- during the dessication phase? Are there any simple universal truths for germination inhibitors? The addition of a water leaching phase for tannins in the seedcoat of fully developed seed in this protocol will of course add additional contamination risk to a complex protocol. Thus I'm hesitant to add too many more variables to an already difficult and time consuming experiment. All advice appreciated cheers DL Share this post Link to post Share on other sites
