Darklight Posted May 24, 2012 My seed were kept in a plastic bag in the fridge since I received them- it's been a while They were sown aseptically and replaced in fridge in media, then put under lights a few months later Not sure how they'd take to having their coat removed and put into a non-sterile area- the seed coat interactions with embryos are complex To remove the seed coat, just hold them in forceps and take the large triangle end off the back of the seed ( there is only one end that shape ) about 1mm. The embryo squeezes out with just a little pressure from the forceps. Mind you this was done here on seedlings that had been in sterile semi-solid media ( agar ) In-vitro the embryos do not like being damaged, not a whit. I lost the best looking one- but there may still be another viable survivor Maybe try just removing the triangle end on a few seed ( not all of them ), and leave the embryo in the coat so it learns to protect itself while developing? Just a thought, best of luck with it! Share this post Link to post Share on other sites
planthelper Posted May 24, 2012 i got two options, do it sterile and with agar or, maybe, i experiment with mechanical and chemical means, to soften the seed coat, or to nick the seed. no rush here, hehehe, great info, cheers. Share this post Link to post Share on other sites
planthelper Posted May 29, 2012 a few years ago, i grew some lago, here is my old pic. http://www.shaman-australis.com/forum/index.php?app=galleryℑ=314 1 Share this post Link to post Share on other sites
pete34 Posted May 30, 2012 i think some species need to go through intestines to get decent viability maybe Lagochilus inebrians is one i am keen for some seeds if any become available let me know Share this post Link to post Share on other sites
Darklight Posted May 31, 2012 Damn. Lost the all seed coat removed embryos after the last subculture. When they're small- especially with the seed coat removed- they don't like any damage. Removing the embryo from the seed coat would cause them some trauma, and even the most gentlest of handling with thumb forceps at subculture would do the same If I try this again I'll add some ascorbic acid to the media to see if that helps Odd, when they're older they can recover from all kinds of browning- but when they're young any browning of tissue at all causes them to hurt Nice to know the embryos are still viable after all this time Still have the mature explants, they've been good for years now Share this post Link to post Share on other sites
planthelper Posted May 31, 2012 i am keen for some seeds if any become available let me know i grew my lago plants from seeds, which you will find mixed in, with the lago herb which get sold... it was common in european head shops at least a few years ago, probably still is. maybe your local head shops stocks it aswell, or you can try to oder from overseas. i think, this plant would suit victorian and tasmanian growers!! Share this post Link to post Share on other sites
toast Posted May 31, 2012 I have several dozen grams (I need to check exactly how much!) L. inebrians herbage available for trade. PM for details Share this post Link to post Share on other sites