Serpent Posted September 13, 2009 (edited) I thought I would post this as I had some success with my method so I thought I would share. After loosing all my spore prints moving, I was a bit bummed as they are kind of hard to come buy these days plus I really liked my variety But I though why not try to get some spores from some old mushrooms I had lying around. Out of a total of 8 250ml rice/verm jars, 3 survived with healthy growth which was what I was expecting. I had 3 mushrooms to play around with, the mushroom were quite mature and the caps had a load of dumped spores on them, i used two approaches to get the spores. #1 Using an exatco knife I scraped the top layer of the mushroom cap into a small tray, were I mixed it with water and drew up into a syringe ready for inoculation. #2 Using water already in a syringe with a needle (drawing up needles make this much easier), I injected the water into and between the muchrooms gills slightly agitating as I injected, make sure you catch the water ha Method two not surprising gave a better chance of survival, one of the jars using this method developed some black mold but was overtaken by the mycelium, but is still under close watch :Phehe I will assume you know to sterilize everything that comes close to the mushrooms! J Edited September 13, 2009 by Serpent Share this post Link to post Share on other sites
cosmofraek Posted September 13, 2009 foaf wishes swim had dried mushroom caps lying around to try Share this post Link to post Share on other sites
Auxin Posted September 14, 2009 (edited) Quite surprising it worked without having to isolate on agar, thats some good luck. Does Oz have a lottery? Over on the shroomery RogerRabbit claims success in cloning from dried fruit body tissue even saying dried tissue can be sanitized with 1/10th strength bleach. I have a jar of dried shiitake slices I may try it with if I get bored, tho I'll go straight onto agar without attempting jars until I isolate uncontaminated mycelium lol Only live mushrooms sold here are portabellos Edited September 14, 2009 by Auxin Share this post Link to post Share on other sites
Mycot Posted September 14, 2009 Another technique that works extremely well is to use gill fragments. Better results than using spores a lot of the time. Agar work is usually the safer way to go regardless of starting material, though I have heard of some surprising results going straight to grain with popcorn probably being more contamn resistant. Share this post Link to post Share on other sites
Serpent Posted September 14, 2009 Ha im not pro enough to have agar but I will look into it this time round! Share this post Link to post Share on other sites