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Found 2 results

  1. Am a huge fan of commercial Trichoderma spp blends as a liquid fertiliser addition to my home garden. The commercial products are usually sold as dry powders- spore mixes treated for storage. I have run right out of these and need some soon. I have Trichoderma spp cultures in my library, and I can easily do liquid cultures based on my standard fertiliser blend. Would the shock of throwing a growing but otherwise sterile culture, even at log phase, into the wild to compete with other organisms potentially any advantage? What sort of optical density of the LC Trichoderma would I need as a fertiliser base, to add as 10x, 100x or 1000x. Can I wing it or do I need to switch the spectrophotometer on? Anyone had any experience trying this?
  2. I'm isolating some local woodloving species onto agar and Trichoderma contam is a constant hassle with one of them. Trouble is the little buggers don't show up as discernable until they sporulate. And the mycelia I am working with is slow to start. What are your best options for working against Tricoderma spp on agar? I couldn't find anything specific on it. My best options so far are Serial dilution- a huge pain in the arse, because Trich is sporulating once it's visible, so the spores go into solution, and there are gazillions of them. Plus a good serial dilution run takes heeeeeeeaps of containers. Over a month. Minimum a hundred. Subculturing ahead- taking only the tiniest fragment of the leading edge, culturing it on, repeat Cardboard slopes haven't worked, as I'm uncertain of this species' ability to grow on cardboard. Still waiting at +10 days to see signs of mycelia. And I've seen Trich grow on cardboard heaps of times. The mycelia would have to grow much faster than the Trich to outrun it and I don't think that's going to happen Is there an agar type media selective against Trichoderma I could try?
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