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Found 5 results

  1. I need to get some samples checked for polyploidy. Apparently the best way to do this is with a flow cytometer. I know nothing of these Anyone here ever used a flow cytometer, esp for ploidy checks? Hoping to find someone who can include my samples in a run or two. Can pool the putative polyploid samples if that makes it easier. Cash or trade- trade preferred
  2. Darklight

    Polyploidy/ mutation

    Was discussing polyploidy and mutation breeding with a colleague the other day and he came out with some experiential statements I've been unable to confirm. He's on leave now so I can't have a longer discussion til he returns. I thought I'd throw the following of his statements out there and see if anyone knows more 1. Polyploidy is cool, and pretty standard stuff in plants. However it's not an increase in secondary metabolites that is the biggest outcome for plants, polyploidy generally first confers an increased immunity to cold temps. Anyone know more? And can polyploidy change the actual phytochemical products of a species? 2. No point looking for gross floral morphology changes in a mutant population, flowers are the last things to mutate as the plant has a vested interest in passing on it's genetics using its current methods. So they protect the flowers, and work to make ornamental variatiations of floral morphology is much harder This one is relevant to me because I was hoping to find floral variations in a mutant population. Haven't seen them. If this line of work is pretty low yielding in terms of results I'll put it on the backburner
  3. Above is the result of the 2014 seedling graft.. .. it was looking like the ones that follow / three little green horns instead of two / .. I'm guessing it's an early sign of mutation .. look in your trays you might find these as well..
  4. Anyone have this one lying round? Specifically interested in Passifloraceae this week No, I won't be using the colchicine. Too toxic for safe use even here Plant Cell, Tissue and Organ Culture (PCTOC) December 2011, Volume 107, Issue 3, pp 451-459 Date: 01 Jul 2011 In vitro induction of autotetraploids from diploid yellow passion fruit mediated by colchicine and oryzalinM. M. Rêgo, E. R. Rêgo, C. H. Bruckner, F. L. Finger, W. C. Otoni
  5. so here it is. I don't know what it is but here it is! any ideas? little conical shoot growing directly from the meristem of one if my trichs...
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