Tripyamine

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About Tripyamine

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  1. oh lordy, moving around fastq files is a right pain thank the lord for nectar and aarnet filestore Beijing genomics institute just/is opening up in aus so sequencing might get a little cheaper here. At the moment its costing me about 3k to do the sequencing on say 12 different "things". I do transcriptomics so for me its 6 different treatments of a yeast and a replicate. Im a bit lucky and we have the illumina, iontorrent and pacbio rigs in the lab upstairs so its just the painstaking mRNA extraction and then library prep but, at that point it gets given to a tech and the magic happens. My research group (if its important) doesn't have to pay for sequencing, however, we have to pay for the kits and the flow cell for the machine so thats what the 3k is. Im lucky and can handle most of the bioinformatics myself so no need to pay for any of that which can get very exxy and ive seen it too many times in the past that they just flat out balls up the analysis and you get bad results, that should be fine but nobody ever double checks because they are 20gb and take a day to crunch at the least. It should be interesting to see how pacbio and nanopore drop the price, I cant wait to run something on a pacbio2 long reads is where its at for large plant genomes so much repeat that make it cooked to assemble.
  2. Yerh I've isolated my own but I'm really looking for an old culture that's been going for a long time I know a few people have em in aus
  3. Hi peeps Wanting to try brew some slick drinks up and am looking for a starter culture of ginger beer plant! I found cultures alive but no stock :/ Send me a PM maby I have something cool you may want! Willing to cover postage of course!
  4. shotgun assembly is shit...... (ie putting a genome together usually gigabases together from 150base reads) As darklight said it costs a lot to build a decent scaffold and get the contigs (long linear DNA) to a small number (closer to the number of chromosomes the better.) Usually you start with a transcriptome (mRNA) to get a feel for expression and whats going on Sometimes mitochondrial or chloroplast genome sequencing is a better idea. You don't really need to have the whole genome, the paper mu! posted is not whole genome sequencing it is plastid or plastome sequencing which is just little dsDNA circles that provied sub functions in the cell like cpDNA (chloroplast) (which is a type of plastid) This avoids the need do assembly as the short reads can be stitched together from previous assemblies (scaffolding) and its not that huge so you can run a few plastomes in parallel (how the paper got so many done) on the one illumina chip which is what the "cost" of sequencing is, other then the couple of 12hr+ days you need to do for library prep if you had a few plants you wanted done (10+) and someone who was good at not fucking pipetting up or contaminating PCR reactions then you could do all of the plastomes for the cost of one "sequencing event" or you could make a snpchip that did it for specific lineages (cactus might be possible), which is based off nucleotides in the dna that change from plant to plant but doesn't really mean much in terms of function
  5. Has anyone ever looked into Light emitting plasma? SOOOO SPENNO BUT
  6. And gas discharge has better peaks! Absolutely nothing wrong with Luxeon T mate (Or the arrays M and K) And seriously check out Bridgelux Vero COBs you will cream your pants, Drive them with LDD-1000's and you there
  7. The big thing with leds is their efficiency. for the most part a T5 fluro has about the same efficiency as chinese leds (epistar and the like) some are upwards of 100lu/watt - where epistar is around 85lu/watt (most t5's are 85lu/watt) If you want LEDs I think you are a fool not to get Phillips Luxeon M or L or K I really Like the K emitters some of these are 140lu/watt Im guessing most of you will use white from 3500k to 6500k for the most part COB's seem to be where it is at now and These little fuckers are the absolute ducks nuts http://www.bridgelux.com/products/vero-series 130lu/watt typical flux and they are in the best mounting package around! Kinda hard to get them in australia You might think I sound over the top here but really spending a little bit more at the start on good LED's will not only last years longer but will cost almost half to operate and put out the same amount of light, or really you should just buy a cheap dual T5 fixture on gumtree, I see 120cm fixtures all the time with ballasts for $5 and they always have hundreds from a job site or some shit Don't buy chinese LEDS they are shit and fluro tubes are better essential
  8. He's still waiting on the seed, be patient it's coming and worth it
  9. Ahhh kinda unrelated but good story none the less I was traveling in the philippines last year with my girlfriend and we were scuba diving around this little rock island a few km out from the mainland, we came back to the island for some lunch and to relax and we were eating some BBQ'd pork the guide had cooked for us while we were diving. Anyway my missus saw the guide feeding bits of pork to some of the little local puppys on the island so she immediately decided she was going to feed the dogs to... she held a bone up and one of the little puppys latched onto it and then jumped over a bit and bit her on the hand just the slightest bit but still drew a little blood. So at this point everyone on the island started freaking out about the possibility of one of the puppies carrying rabies, so one of the old pinoys minced up a few garlic cloves between his fingers and raked it into the bite then rubbed it off and rubbed more fresh garlic on, this went on for 20min and she was not happy about the stinging, he then taped a piece of garlic to her finger and sent us on the boat back to the mainland. In the proceeding 2 months she had about 15 needles just to make sure But when I looked on good old wiki, apparently it is the first port of call for a rabid bite
  10. I use a $20usd (shipped) blue plastic probe from Aliexpress, this is wired up to a simple pH circuit and fed into an ADC and to an Arduino monitored via the serial out This sits in a saltwater frag tank all day and the data gets logged to a xively feed; when the lights come on there is a nice shift from the photosynthesis kicking in, and it's all exactly the same as the colorimetric 7 to 8.9 test kit that I have which is highly regarded (sailfert) As for probe calibration, Im sure you're aware that 99% of calib is done by cleaning the probe/keeping it clean I use a 12 bit ADC and this generates a value between 0-4096 which is directly proportional to pH The main calibration factor used is drift, which is the measure of how different the probe's reading is to a "new" probe if that makes sense
  11. Probably because of the commercial value of some of the other products derived from reticuline/thebaine Just remember that the hoo-har is that there are essentially 3 magic yeast's -1 that can make (S)-reticuline from glucose -1 that can convert S to ®-reticuline -1 that finishes up the job and goes to thebaine or further So now it's just a matter of time for someone to combine all three.... But it's not that easy, there are complex regulation difficulties that would need to be overcome
  12. Fun stuff http://www.theguardian.com/science/blog/2011/jun/21/scientists-make-lsd-from-microbes But if you get the biochem from the yeast -> opium paper (which it only kinda is - more about a cheap pathway to a general precursor) the scientists managed to beat the biosynth that poppies do in terms of steps/complexity
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