Jump to content
The Corroboree

Auxin

Trusted Member
  • Content count

    4,087
  • Joined

  • Last visited

  • Days Won

    19

Everything posted by Auxin

  1. Auxin

    bacteria in mushrooms

    Dont know if its true or not, but it sounds like a good reason to pasteurize and dry your mushrooms. Even if it is scare tactics that kinda stuff CAN still happen. Ya only got one liver and a new one is VERY EXPENSIVE! And thats if you live long enough to make it to the top of the liver transplant list.
  2. Auxin

    Urine - recovery of metabolites

    "Isnt the active ingredient Muscimol whilst muscarine is the undesirable toxin"- reville Oops left that out, yes muscimol is the prime active ingredient and its an alkaloid, but not only is it not that stable to strong acids or bases but it is also quite polar thus making a/b extraction hard with the normal 'non-polar' solvents available to home experimenters (even ether wouldn't be that effective, especially with the tiny amount that would be in pee, even if you salted the pee most of the freebase alkaloid would probably stay in the aqueous layer), it is also highly toxic in pure form. Also while muscarine may be a undesirable toxin by itself, in combo with muscimol one mans toxin is another mans entheogen spice. Just like the peyote ritual where the 'toxins' cause you to through up, many find that to be an integral part of the experience. "Drinking Pee after tripping on Psilocybian mushrooms does work."- reville Interesting! I wouldn't have expected that. Any idea what percent goes through unaltered? Does your gnome get a full strength trip off pee from a full strength trip or is the potency notably diminished? Anyways, in regards to purifying a single compound out of pee, no mater what you were after (aside from urea) youd need many liters of pee and the process would no doubt involve multiple extractions, ion exchange resins, column chromotography, maby activated carbon, vaccum dessication/sublimation/distilation, forming chemical derivatives to aid purification, then regenerating the original product, heavy metals, etc, etc... Technically possible (has been done many times in professional labs mostly with the pee steroids) but HARD, costly, and time consuming.
  3. Auxin

    Urine - recovery of metabolites

    A. muscaria has Muscarine which is a quaternary amine, and it has the alpha amino acid (zwitterionic) Muscazone. Remember they were talkin 'bout feeding 'em to raindeer and milking the urine. I dont know how active muscazone is or if it would get through unaltered, but muscarine does so thats why people drink the pee of people trippin on A. muscaria. And your right, as soon as psilocybin (zwitterionic, non-CHCl3 sol.) hits the stomach its converted to psilocin (of course its probably a mute point because it would probably be metabolized at least to 4-hydroxyindole-3-acetic acid before bein peed out, and that aint a entheogen [but it could be an active auxin])
  4. Auxin

    H2O2 fungicide

    H2O2 is not just a fungicide, its a biocide! It kills all living tissue. With larger (or more well protected) seeds it is possible to use a breif treatment with 1% or less and not kill too many. Any H2O2 will kill cacti seedling roots. Real fungicide is best.
  5. Auxin

    Urine - recovery of metabolites

    Been watching this topic and my feelings can be summed up into two words: WHAT THE? -Recovery would be more costly than just buying more. -Why pass the A. muscaria through an animal, why not just use it, or extract it as is? -You would run into more toxic metabolites than desired product. -Some of the suggested materials contain quaternary amines as the only amine, or they are monobasic & monoacidic zwitterionic substances. Neither can be extracted by A/B methods or standard 'non-polar' solvents. -If your gonna play with pee, why not make pheromones out of it? The potential result is much more pleasant and you would have an interesting story to tell any resulting children ("How did I meet 'mommy'? I met her by smearing my body with chemically modified urine concentrates!")
  6. Auxin

    Mescaline + Syrian Rue

    I have heard conflicting reports concerning the combo, so proceed with caution. If one were to do such a study it would be important to use pure synthetic mescaline rather than the cacti-derived stuff. Most cacti-derived 'mescaline' actually has many other alkaloids mixed in which could alter the effect. Also important would be to use a greatly reduced dose at first just in case of extreme potentiation, or any toxic effects. Also remember that many commercial sources coat syrian rue seed with poison to 'deter' people from using their product as a drug (the bastards would rather send a person to the hospital than allow them to practice their religious or recreational rites.) And of course all the MAOI rules apply. No tryptamine containing foods, no alkaloid containing foods, etc... And of course the standard disclaimers: IM NOT A DOCTOR EXPERIMENT AT YOUR OUN RISK EXPERIMENT ONLY WHERE LEGAL ETC...
  7. Auxin

    Be Pal, help me with an assignment

    Genetic engeneering of plants can be a good thing. Many asian babies would get sick and go blind because ordinary rice has a harmful protein in it. The genetic engeneers steped in and stoped the rice from producing that harmful protein, and they made the rice produce beta carotene. This 'golden rice' is now good for your vision! GM plants can also be made to produce valuable drugs, for this they currently normally use animals or E. coli (the good kind) instead, but plants are much easier- no sterile bacterial culture, no damn cows, just plant a feild and watch the medicine grow. Of course, like anything, it has its dangers. Genetic engeneering is like a scalpel, in the hands of a fool it can only do damage. But in the hands of a well trained person it can save lives, but of course now and then even the well trained person can slip and do more harm than good. Maby we have enough food now, but what about when we pass the population 10 billion mark? Its not to far off. Also what about space food? If (when) we build colonies in outer space we will need crops that produce a maximum of nutrition and oxygen with a minimum of space and water. As with any advanced technology there is a initial fumbeling around stage. Thats where we are now. We can not yet write new genes, we can only find them. As the technology gets more advanced we will be able to properly engeneer plants to do many important functions and save many lives, but the technology cannot get more advanced if it is abandoned just because of a few mistakes, or even worse- because of simple fear. The first people to research radioactive materials died from it and many wanted to abandon research right then, but now radiation is used routinely in the worlds hospitals to save lives (not to mention in dentists offices to save teeth, in airports to stop bombs, etc.)
  8. Auxin

    Increase in sensitivity to caffeine

    "as soon as you tell ppl you work in a scientific field the conversation falters and they run away. You get that?" OH Yeah, I get that static. Thats why I now talk to beakers, test tubes, plants, etc. more than I talk to people. But then you get emotionally attached to the beakers and its hell when they go away. I lost one of my favorite girls just a few days ago, a sweet old half liter graduated HEAVY walled pyrex beauty that graced me with her service for the last four years of her life. Dead at the tender age of eight to ten! She will be missed. At least my Vigreaux columns haven't left me yet, now That would hurt.
  9. Auxin

    Testing Seed Viability

    "And what do they adjust the pH of TTC with?" Glad you asked, the buffer adjusts the TTC to a final sol'n pH of about 4.9 Check this out: http://www.agro.agri.umn.edu/plant-tc/list...1/msg00048.html I presume that the buffer is to optimize the TTC test by making sure that the test reagent is not so acidic as to kill off more sensitive cells. Remember, this specific test was engeneered for microscopic (post cryo) cell culture testing, maby the buffer is not used in seed tests because seeds have so many cells that even if the TTC kills some cells in a viable seed, it wont kill 'em all, so you still get a positive result. Or maby the dudes were just aiming for the pH of a specific ringer sol'n or something and that info later got filtered out somehow. The plant-tc archive has a fair amount of info on TTC tests if you have the time to go through it.
  10. Auxin

    Increase in sensitivity to caffeine

    I dont really think your liver alarm is going off (be advised I'M NOT A DOCTOR). What I think is that your body was habituated to a certain level of caffeine and then you reduced the amount and after a little while your body adjusted to the change. Then when you had lots more than usual everything above your current habituation level (everything past the first few cups) caused the (over?)stimulating effect. On the subject of funky livers, have you got into milk thistle seed? Its got several odd-ball flavones in it that do wonders for the liver. I use it as a preventative measure because I'm a chemist and some of those common solvents and reagents cause damage to the liver (why do the GOOOOD smelling solvents have to be hepatotoxic? Now OSHA wont let me roll around naked in my chem lab smearing isooctane and naphthalene all over my body- damn that sounds good. Mmmmm Naphthalene Sweeeet Isooctane Now you see why I'm on a computer and not out on a date. Organic chemistry is a Very Rare fetish and it scares alot of people off.
  11. Auxin

    Testing Seed Viability

    YEAH, I do the 2,3,5-triphenyltetrazolium chloride (TTC) test on some of my seeds. Works great. In my experience you DO NOT want to slice up the embryo. If the seeds are small and white (like capsicum seed) you can use the whole seed, but if the seed coat is a dark color (like watermelon seed) you should cautiously remove the seed coat and do it that way (removing the coat before the test makes the test go faster. But scaring the coat before the test, and removing the coat after the test (after the seeds swell, don't use non-swollen seeds) is far more accurate because it minimizes pretest stress and false positives due to physical damage and bacteria. If you REALLY want to you can use just the embryo, but the embryo must not be exposed to air for more than a minute before placing in the TTC solution. I use 0.1 to 0.25% TTC for my simple seed tests. Some dudes use a 0.05 M phosphate buffer at pH 5.80 with upto 1% TTC for cell culture testing. And if your testing pollen viability under a microscope I have been told it is required to use a filter sterilized sucrose/TTC solution with some incubation to activate pollen. Also, some alternatives to TTC have been developed. One more common one is MTT [3-(4,5-dimethylthiazolyl-2-)-2,5-diphenyl tetrazolium bromide] This is often used for the above mentioned pollen test. I have never used MTT, so don't ask me how well it works. Any questions?
  12. Auxin

    PIHKAL and Phloridzin

    "I can only find refs to it being a tonic."- Torsten Phlorizin: "Formerly [used] as antimalarial"- Merck Index 8th ed. "Suggested as a test for kidney function and has also been used as an antiperiodic, but it is more dangerous than valuable"- The Dispensatory of the United States of America 1960 Edition
  13. Auxin

    Savaged Lophs!!!

    To get rid of the cats you could cover the area with chili powder (not to much at the very base of the cacti though, that could promote rotting.)
  14. Auxin

    Ashoka

    Ashoka, I tried to respond to your email concerning fatalii seed, but the mail was returned as undeliverable (probably just an AOL foulup, that happens sometimes) so I thought I would post my response here: I've only saved two dozen seeds so far (from the first harvest i.e. lower viability) because I have not yet got around to protecting them from crossbreeding with my other peppers, so those seeds may well be a cross between fatalii and chinchi uchu (or maybe caribbean red or something else but chinchi uchu is the heavy pollen producer). So if you like gambling, sure I'll be willing to trade. However, if you want a set of genetically pure seeds you can buy them here: http://www.reimerseeds.com/item.jhtml?UCID...619&PRID=346790 Oh... If you do start growing fatalii, heres some useful fatalii trivia. About 200 years ago some slaves were repatriated and before leaving the americas they stuffed their pockets with some hot pepper seeds. Some of the seeds miraculously made it through the long sea voyage and were planted in three or four african countries. The seeds that were planted in the area that is now the central african republic evolved into the modern fatalii. So now you know- something good actually did come out of slavery! [This message has been edited by Auxin (edited 28 May 2002).]
  15. Auxin

    nutmeg oil extraction

    If you've got lots of that mixed oil you could purify it by saponification. The goodies are in the non-saponifiable fraction. Butane extraction is very dangerous, and so is the product if your not careful. As a wise man once said: "It can easily kill you."
  16. Are there any Capsicum species native to Australia? If so, are there any good hot rare ones that you know of? If so, where would I find the seeds? I want to breed a hybrid that represents every continent on earth (except Antarctica of course), so far I have ones from Africa, Europe, and the Americas. Asia will be easy, but i've never heard of Australian ones. Any info will be helpful. Red ones are best (more vitamin A & C). Thanks Ok, I'm told I mis-spoke. There are no truly native species anywhere but in the americas, it is an introduced plant everywhere else. What I want is a purely australian variety of C. chinese (prefered), C. annuum, or C. baccatum. I am also told that my best bet would be New South Wales. [This message has been edited by Auxin (edited 20 May 2002).]
  17. Auxin

    Australian Capsicum

    I posted this elsewhere but some people just dont go into bushfoods I guess. Im looking for AUSTRALIAN VARIETIES of Capsicum sp. Do you know of any? If so, where would I find the seeds? I want to breed a hybrid that represents every continent on earth (except Antarctica of course), so far I have ones from Africa, Europe, and the Americas. Asia will be easy, but i've never heard of Australian ones. Y'all grow 36,000 tons of commercial peppers, so surely a new 'Australian' variety has popped up by now. Any info will be helpful. Red ones are best (more vitamin A & C). I want to rejoin all races of hot peppers, a truly globalized pepper for a globalized world. Peppers of the world unite and revel in a glorious future of mouth burning, brain melting pepperdom, free yourself from the confines of racial pepper preferance and move into a new millenium of pepper equality and cooperation. If your master is forcing you to breed with your own kind against your will, jump in the mail and come to the USA- Land Of The Free, And Home Of Pepper Equality. Once peppers unite the world may follow, long live the pepper revolution! Thanks
  18. Auxin

    Australian Capsicum

    "I believe habanero come in 'bio-weapons grade'"- Adrian I dont know about biologial weapons, but it makes for one hell of a chemical weapon. One time I made chili sauce that was from 20 to 40 times the strength of Law Enforcenent grade pepperspray, Mmmmmmm. For some reason no one else would eat it though (more for me ) Normal Habs range from 80,000 to 200,000 SHU. Red Savina is reported to get around 570,000 SHU. My hottest hot sauce gets to around 2,000,000+ when I prepare it in my chem lab. "Does anyone have any new information about chilies and endorphin release?"- Adrian Sounds like you want the Fatalii pepper from the Central African Republic. I grow that beast, its reported to be 100,000-300,000 SHU but it doesn't work quite like the normal ones. When I eat it I dont feel the hot at first, in the first second or two my skin flushes, my scalp starts sweating, and theres this warm fuzzy euphoric melting sensation in my brain that takes 15-20 minutes to fade away. THEN, in second 2 or 3, THE HEAT HITS. Its like being plunged into those burning lakes of sulfur the christians obsess over. I love it, if thats your hell I should become a sinner. Muggers can have my money, but they have to fight for my Fatalii. [This message has been edited by Auxin (edited 25 May 2002).]
  19. Auxin

    Amyl Nitrate

    "purchasing *any* chemical from a chemical supplier without some sort of acceptable usage story is risky."- Somnif If they ask what you intend to do with it, you are already in trouble! I dunno about Australia, but in the US chemical companies make weekly calls to the DEA, ATF, etc. My last order was for 2-naphthoxyacetic acid and benzylaminopurine, totally innocent stuff- but they still treated me like a freakin criminal (and that was BEFORE the september 11 BS.)
  20. Auxin

    nitrogen fixing bacteria

    When rhizobium bacteria form a node they are changed to rhizobia bacterioids, and can not be changed back to rhizobium. That means blenderizing nodes will not make a good innoculant because it takes an active rhizobium bacterium to make a new node (also it would promote rotting). Rhizobium are supposed to be ubiquitous in soil, so I would try making an inoculant by mixing numerous soil samples with water, decanting, and use that to water the seedlings. Thats my theory anyway, I have never tested it- but maby now I should.
  21. Auxin

    Amyl Nitrate

    Torsten: 1) The decomp of nitrous acid is slower at lower temps (because it is more soluable, when you produce more than can be dissolved then the excess goes away as nitrogen oxides), but the main factors are the speed of mixing acid to metal nitrite, the thouroughness of mixing while doing it, and the quantity (not to mention identity) of solvent used. Remember, nitrous acid ONLY exists in solution. 2) The source of the stink you mentioned could be nitrogen oxides, or a decomp product caused by mishandeling the sulfuric acid, or the always frightening OTHER. 3) I don't always know how non-chemists do things (I was born a chemist, seriously. I made a homemade azo dye from my stomach medicine and phenol before my middle school even got around to talking about pH! Thats how I learned everything in #1) but their probable mistakes would involve using plain water in all the washings, waiting too long, leaving the mix in the light in a warm room, and other assorted mistakes. 4) I wasn't talking about residual water in the sense of droplets floating around, but instead I meant water disolved in the alkyl nitrite (MANY organics do that, although being 'non-polar' (a deceptive term) it is not uncommon for organics to have the capacity of solvating anywhere from 0.1 to 5% water. Even benzene can have a moderate amount of water disolved in it. Alkyl nitrites react with water forming nitrogen oxides and the parent alcohol (just remember that they are esters of nitrous acid and an alcohol) 5) HCl would have different problems depending on the procedure. It would definatly decrease yield (Its a wet acid rather than the common drying acid- 98% H2SO4 (for esterification you want drying) and it is a weaker acid catalyst) If he really messed up some HCl could end up in the product (I have burned my lungs with HCL gas several times- In five minutes you sound like you had just smoked 30+ years worth of cigars! Scares the hell out of my boss, parents, and myself.) 6) Your right, alkyl nitrates make a nice big bang. Glyceryl trinitrate is the most commonly known one, however most people wrongly call it 'nitroglycerin'. OK I think that covers everything.
  22. Auxin

    Amyl Nitrate

    Torsten, you have never made azo dyes have you? That nitrous acid breaks down to water and nitrogen oxides, If your careful the occurance of that is hardly worth mentioning, but if you are off by a little bit in any direction it starts to stink bad. Also true is that the product can be cleaned up by washing it if you do it like a chemist would, otherwise you either destroy all your product or only remove some of the contaminants. Plus the product would contain residual water, so you would have to dry it with Na2SO4 or CaSO4 (in the absence of a fume hood the experimenter would then overdose). And the pure pharmaceudical stuff has been distiled (Overdose+BOOOOOOMMMMMMM=dead) Besides I have never seen amyl alcohol or isobutanol sold in stores, so the home experimenter would probably end up trying to make Isopropyl nitrite (WAY too explosive and volatile to use as a drug).
  23. Auxin

    Amyl Nitrate

    Yes, amyl nitrite is an alkyl nitrite. And no, you shouldn't even think about making it. You would probably overdose off the alkyl nitrite fumes, have your throat and lungs irritated (or mildly burned) from the fumes of various nitrogen oxides, and the alkyl nitrite vapors can EXPLODE if it finds a spark. Plus the product may be contaminated by toxic and corrosive nitrogen oxides and would have a very short shelf life due to contaminants including water, air, etc. In my humble opinion alkyl nitrites should only be made by organic chemists, and then only for non-drug or prescription uses (great for making azo dye intermediates, heavy metal indicators, pharmaceudicals, and many more.) My 'ANTI-DRUG' is organic chemistry, I couldn't bare to waste a good alkyl nitrite on getting high, not when pretty azo dyes and experimental plant hormones could be made!
  24. Auxin

    Amyl Nitrate

    Amyl Nitrite info at RxList Side effects/interactions info: http://www.rxlist.com/cgi/generic3/amylnitrite_ad.htm Overdose info: http://www.rxlist.com/cgi/generic3/amylnitrite_od.htm
  25. Auxin

    Native Capsicum?

    "I was just going to say, capsicums are really only from the Americas."- Ed Dunkel Correct thats why I corrected myself and said an Australian variety. When Capsicums are introduced to a new reigon they mutate/evolve into new varieties fast. Capsicums now grow on every continent except Antarctica. I guess I put this in the wrong forum. Also correct is that the green ones are just immature, however the mature ones can be red, orange, purple, yellow, white, etc. OH, just in case Torsten reads this I have found the answer to why americans call capsicum fruits peppers: Christopher Columbus. All his lies are perpetuated every generation- When he came over here he was looking for a source for black pepper, the closest he found was some red capsicums so the fool named it 'black pepper' and took it back to the queen. Well being red they dropped the black and just called 'em peppers. If that sounds too far out to be the truth remember this is the guy who was looking for India when he found the americas, it was clear that this wasn't India, but he called it India and to this day the Native Americans are refered to as Indians (When you catch an American doin' this, ask 'em what they call people from India, I have never got a good answer.)
×