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The Corroboree

Darklight

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Everything posted by Darklight

  1. The eggs are really nice in a salad with avo, maccas, greens and a bit of chilli in the dressing Yeah was thinking about the bug eating aspect. If I ever get around to building another fort knox for some I'll be putting oyster mushroom buckets on bricks, hopefully the quail will eat any of the tiny flies that the mushroom buckets attract Are they financially efficient? Reckon you get your moneys worth out in eggs? And are you planning on raising more from the eggs?
  2. OMG. That's fantastic. Mine didn't even last three months and yours are still going I think I have quail envy :D
  3. Darklight

    MELB MUSHROOM DAY THIS WEEKEND !

    I'd go if I was in Melbs for sure :D Look, if you've ever been to a mushroom tek and feel like you haven't learned enough to be as productive as you like, go to another one! Mycology's quite the thing to wrap your head around- even with years of plant growing and plant biotech experience it took me a coupla years work to get the gist of the different schedulings and life cycles, spawn implications etc. Luckily the teks taught these days are way simpler than they were back in my day, and we know which strains are better suited to local conditions and substrates. But it never hurts to do a basics workshop again if you're struggling ( ID workshops I always always learn new things, I try not to miss those ever )
  4. Darklight

    Cultivation of glowing mushrooms (ABC news article)

    LC of Panellus doesn't glow IME, but I did only try it in one media. Other media could work
  5. Darklight

    Whats wrong with this Loph?

    This. Exactly. Do not throw more than one treatment on any sick plant at a time unless you have experience with that combination and that species. Give the treatment time to work ( or fail ) before throwing anything else at it You might lose your plant anyhow, but you're giving it a better chance in the meantime. And if it fails, you can tell people that specific tek didn't work- for your species and pathogen, in your area. Which will help others My suggestion of Trichoderma has only worked for me thus far, for pathogens in my area, on cactus I've previously had problems with. I've not yet enquired if the protocol has been tried on cactus in other regions by other people. I'd expect anyone throwing Trichoderma as a new treatment for a sick cactus in another bio-region would regard it as an experimental treatment. My extensive experience in people telling me what works for them has shown that about 80% of people throw a bunch of random stuff at their sick plants, in an entirely undocumented and cavalier way contrary to any instructions, then pick one thing from the bunch and blame it for the success or failure. It makes for unreliable data. Yup. Lophs here in NNSW can stand being pulled out of the ground over winter to dry out then repotting, but not sure if I'd recommend that til yours has at least started to heal- or even fully healed
  6. Darklight

    Whats wrong with this Loph?

    Yup, as a foliar spray It's a coarse sharp sand blend, I think it was commercial. Planted it in that pot about 15 years ago Yup. Mostly via what falls on the soil from the foliar spray. Ideally I apply it once every 1-2 weeks when I foliar spray everything here
  7. Darklight

    Whats wrong with this Loph?

    Dunno if it'll work in your climate but I throw a commercial Trichoderma solution over anything that looks wounded, mouldy or is prone to it. Early morning, so it has a chance to dry, absorb, multiply before the cool evening sets in. I've never been able to grow Lophs outside, nor Mammillaria until I started doing this about 18 months ago. They both always got orange rot 100%. Last growing season was my first ever successful button cactus year. Even late and cool in the season, when a bird pecked a massive hole in the big Loph it healed fully within 48 hrs, callusing started after 12hr after application. No further damage, no pathogens moved in IME Trich regular applications also stop larvae if you always respray within the breeding cycle But that's just me, here. Don't use it as a magic bullet for everything without testing on a few of your babies over a season or two. Was gunna make up a commercial mix and sell it, but my marketing skills suck. Get yours from a hydro shop. Have also posted on other threads here on Trichoderma
  8. Darklight

    Yukutlan (RFB)

    I really like that transparent grow box in the pics- did you make it or did you buy it?
  9. Darklight

    IMG_20181018_092359.jpg

    Oh wow, so beautiful :D
  10. Darklight

    Wanted: Compound microscope/ trade

    Niggles and I have been looking at microscopy, learning more about it. Um, there's a lot more to it than we thought... What's become obvious early on in the piece is that my cheapo $50 ebay scope isn't up to the job of looking at stomates and spores. It's not just the magnification, it's the crap optics. Need a compound scope with illumination, pref LED. Pref with coarse and fine focusing. Magnification to 1000x or more if there's an immersion lens involved ( never used one, no idea how that works ). I wanna see stomates, chromosomes, spores. Good optics are essential. We'll be attaching a camera to it too. Zeiss. Leica or Olympus lens or similar quality. If you have a good compound scope at home just sitting round and you'd love to see it get used regularly, gimme a hoi and we'll find a suitable trade for you.
  11. Darklight

    Cultivation of glowing mushrooms (ABC news article)

    I'm lichen that one more and more Same same. Though there might be one here trying to fruit on the agar plate ( could also be contam, discolouration from inoculation point tho and never seen this particular effect before ) Will post if it does fruit
  12. Darklight

    Microscopy and DNA workshops

    How did the microscopy workshop go? Wish I'd been there. Starting an informal microscopy study run today with another forum member to try and get some basic teks in hand to add to current research. Is way more daunting than I thought. Microscopy, like tissue culture and TLC, is an entire field of knowledge of it's own First lesson is cleaning our microscopes. It's mouldy in NNSW and even the best stored scopes cop it We're starting with the cheap-arsed compound scopes we've had for years ( USB camera, ebay ) to see how far we can push their utility before we look at the worth of saving for upgrades. Tho good optics and lens' are important- I once used the USB camera on a 1920s microscope with immaculate optics and the results were absolutely brilliant- way better than the cheap arsed ebay one I was comparing it to
  13. Darklight

    Flowhood from ebay review

    Yeah nah, I wouldn't. Says it's a vertical flow cabinet- so clean air is pushed from the top of the cabinet to the bottom Best flow cabinets for general contamination free cultures IMO are horizontal flow. Clean air is pushed from the back of the cabinet towards you, so if any contaminants are inadvertently transferred during work they will be blown away from the work surface towards the user Anyone here ever used a vertical flow cabinet for routine sterile culture transfer work? This link says it's possible, but I've not seen it in practice ever https://www.thermofisher.com/nl/en/home/references/gibco-cell-culture-basics/cell-culture-equipment/laminar-flow-hood.html
  14. And as long as there are no competitor organisms and the humidity and temps remain in the Goldilocks zone for that species etc etc Actually that's a pretty cool question Zool and I don't think the answer has ever been found for all species. Some form underground storage organs like sclerotia which are basically compressed hyphae and hang there til conditions are right to keep growing, some species are always present as mycelia when they grow in symbiosis with trees, and whatever it is with the pseudosclerotia that some fungus form I do not know at all.
  15. Brilliant response, thank you! I got caught up with soil chem possibles and didn't answer the question proper Great to hear from an expert
  16. Everything about that dream is hilarious, thanx for sharing Scary that you have Tony Abbott in your head, but lovely that you are feeling sorry for him despite the ick factor. Obvs you have an outstanding subconcscious :D
  17. Darklight

    Fungus killing worms in my garden

    It will. Is it still happening? Relax and let the fungus do it's work, burying them is overkill and just means you work so different fungus can get them, only you don't have to see it The fungus will take us all one day, as will the bacteria and all the other little critters. Is how it's sposed to be
  18. That's depressing, but an excellent point. I switched to individually wrapped dishwashing tabs in my lab dishwasher cos the unwrapped ones would physically degrade over time, but I'm also aware of any potential impact ( less likely on the actual glassware cos after washing it's rinsed twice before drying ) of both detergent and packaging. All a manufacturer has to do is show best practice for the recognised and available technologies under legislation. Whether the govt or industry should monitor all degradation products/impact is a non-issue, because they're simply not going to for every product til it crops up in the news. It's finally up to consumers to trust that standards are continuous, transparent and honest Look, seriously the only way to work this stuff out is to do it ourselves. And even then the best experiment design won't replicate the infinitely complex soil and other environmental co-factors. There's not only bacteria, and enzymes, but also microfauna, fungi, slime moulds and things we probably haven't discovered and can't consider as cofactors yet If you're passionate about this and related issues it'd be worthwhile to consider degradation products, and set up a working protocol to analyse and share results A basic ( ie facile, simple, thorough but somewhat incomplete ) experiment design would be trivial, funds for a thorough chemical/ microbial analysis of each sample could be more of an issue, but the biggest issue is the logging over time, the synchronisation of standards so that others could repeat the experiment, data integrity, centralisation and multiply redundant ( ie backed up safely ) storage and finally publicising results so they can be understood and critiqued by a wider audience It's also a possible backbone plan for a Roundup ( glyphosate ) experiment Citizen science. It's something everyone can do. And it means you really give a fuck, not just an FB like
  19. Darklight

    Fungus killing worms in my garden

    Nice question! Could it be that the cow poo came from cows that had recently been drenched and it mightn't be the fungus directly killing the worms but the cow poo instead? Personally I wouldn't worry about a garden fungus, the weather changes to favour whatever appropriate species is around and I find that single species generally don't predominate for long before they're replaced When I find garden mycoflora that appears to be damaging my plants long term I hit the area with a soil probiotic spray and do the plants as a foliar spray as well. Repeat apply for a few weeks to see if there's results. They're organic inspired, most contain a blend of beneficial bacterial and fungal starter colonies so there's always something in there to reclaim the ground, roots and plants from pathogens
  20. Darklight

    Microscopy and DNA workshops

    I so wish I could go to both these workshops, they're both critical skills I'm missing and a colleague was telling me how great Bioquisitive is when I was at Garden States last weekend Alas, I live nowhere near Melbs
  21. Darklight

    Vale ReshroomEd

    Last year, around the time of EGA in Melbs, one of our long term and seriously valuable members died. If you have an Eileen clone, or have drunk of the Eileen clone, you've been a recipient of Ed's work. And probably his generousity. If you ever did an Otways camping trip with him you'll know why his death is such a great loss. I only found out today. We'd go months without speaking or writing then binge on it with hours of gorgeous, insightful, funny, irreverent conversation. We'd have phone drinks and pass out talking about amazing shit. He'd pick everything apart, then I would, and we'd put it all back together and it all made sense. He'd been sick for years- really gut wrenchingly sick, and he hated speaking to anyone when he crook with a pathological loathing I can only be impressed by. So we gave each other space. Only this time I gave him too much. Fuckit. My head is full of Ed stories. There are no favourites, they're all gold. But the type of gold which fades in text. You had to be there. He named the Eileen clone after his mum, and sent that cactus clone round the world. Often twice as much as anyone had paid for, and often for free. When I asked him how his mum felt about the transubstantiation, being symbolically consumed by complete strangers at 75, he shrugged and said "Grouse. Ask her " Nobody grew wasabi like Ed. Reading his old emails is like hearing him speak all over again. All the words got used. The big ones, the small ones, the swear ones, archaic words, technical ones, words used in contexts I'd not heard of and words I had to look up, side by side in every sentence Vale fearless bloke, irreverent gentleman-scholar, hunter, mate, plant fiend. You vainglorious bastard, I loved you like you were torn from my own rib. You brilliant, stubborn, sophisticated, gentle, resourceful, skilled, compassionate, staunch, generous, insane prick. May the next place you're in find you free from all pain
  22. Darklight

    Vale ReshroomEd

    He so would. It'd be logged by some old unit he got off ebay and hacked into workable with fencing wire. Was gunna say 'baling twine' instead, but Ed would never hack anything with flimsy, and he'd organise proper documentation :D It was his birthday the other day, I didn't miss it but was too gutted to post Fuck he woulda loved the last EGA, then we woulda taken the troopy somewhere steep and stupidly unsafe so he could show me a plant and then we'd drink whiskey or whisky So many lovely new people, so many lovely old people, and still there is an Ed-shaped hole in the universe May we all be his greatest experiment ever <3
  23. Am always fulla *something* lol But at EGA I am always full of joy because EGA, you lot create the best vibes
  24. Darklight

    Plant Swap Melbourne EGA

    EGA Plant Swap was lovely chaos in the best kind of way! So many people, everyone was respectful of the social bond around the sharing, people without things to swap were respectful and many of these people walked away laden with plants and with all parties happy. A few very generous people ( I have no idea who, so shout out if it was you ) turned up with HEAPS of plants to share, which got shared, and I hope they found some of the species which needed them too People milled, plants vanished, moar plants appeared, it was a whirl Did not get enough time to be properly helpful or even see who was around and what was moving, I got to talk to a few lovely individuals about mycocultures I bought with me and got sidetracked, sorry Amz and team Many thanks to @Amazonian for co-ordinating this and also for keeping the vibe cool and stopping entropy in the face of utter chaos Many thanks to lovely EGA crew for allowing the space to happen
  25. Darklight

    regrowth study in 2019

    Sorrow at the destruction of plants, population, association and habitat Thanks for your hard work, and for those who help with it Joy that you are continuing, that somebody cares and does the work so well Wishing you more power for your species and for your eagle eye data
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