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The Corroboree

mimzy

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Everything posted by mimzy

  1. mimzy

    Is This Asbestos?

    It's impossible to tell from macroscopic photos if a material contains asbestos or not. Wear a mask, take a small sample and send it into an accredited testing facility.
  2. mimzy

    Piriformaspora indica

    You'll also need an DOA import permit, so add another $300 on to your costs.
  3. You shouldn't find Trich sp. inside stipe or cap tissue, but you will regularly find pseudomonas which can be easily cleaned up with amp. I also wipe down my specimens with h2o2 and tear them open rather than cutting. This is important because often contaminants can be introduced into the the tissue by the knife as it passes through the 'dirty' surface layer. Also, where is the contamination popping up? If at the inoculation site then this would be consistent with specimen contam, but if elsewhere then maybe your plate wasn't sealed? With love, Mimz
  4. Yes they are no longer selling it, but bunnings still has stock they refuse to take a loss on. Perhaps you could forward to bunnings?
  5. mimzy

    Mushroom ID please

    Great photo of the gills by the way.
  6. (1) For the love of all things good in this world, do not store more grain than you have to; or at least store it properly. For most of my commercial cultures I use organic wheat grain. Some time ago I bought 30kg sack, thinking I would cut down on costs by buying in bulk. The first sign of trouble was a few pantry moths flying around the lab. No problems I thought, I'll leave the sack out in the sun for the day to kill off the weevils and store it in another room. Well, it turns out the sack was infested, and with the humid warm conditions of late summer, I soon had 100's of moths in all my rooms, in all my medias, in all my grains, in all my supplements, in all my everything! (2) Put your agar plates in the fridge as soon as they have set. Leaving agar plates out on the bench for extended periods of time gives mites (YES MITES) a chance to crawl in, contaminate your plates with asper. sp and/ or lay eggs that will ruin your culture later. Don't try to save your plates; freeze them to kill the mites and throw away. Again, this time of year is particularly bad with high humidity. (3) If you can avoid it, don't have any timber in your clean space- given enough time and moisture you can have a mold city growing under benches, which you wont know about, absolutely loading the air with spores. Keep it stainless or plastic. Learn from my mistakes fellow mycologists. With love, Mimz
  7. mimzy

    Mimzy has learned the hard way.

    The only way I know of recovering a strain from mites is to slow freeze your culture in 15% glycerol /water. Freezing will kill the mites but your cultures should be ok. I was lucky most of my plates were sealed with parafin tape and avoided disaster. Any new clones from fruit bodies collected el-natural go straight in isolated eskie until I can confirm they are ok. It leaves in awe of how finely balanced our ecosystems are; in the lab its always one victor over another... whether thats mold, mites or moths.
  8. mimzy

    Sublilacina porn

    When it rains, it pours hey. Fantastic work Zen! Can't wait to get this one into culture.
  9. A field guide to Australian fungi - my first and favourite guide book. Brings back great memories of tramping around the forests, learning every fungi in sight.
  10. I had a dactylium in mine- proved it with aseptic agar culture. When I emailed them, they already knew about the problem but hadn't recalled it because they are too cheap. Apparently they are no longer producing the grain, or importing it...
  11. mimzy

    Lepista sublilacina purple mycelium

    Awesome work Zen. Did you have any luck fruiting your indoor peat-cased cultures?
  12. mimzy

    Laminar Flow Hood on G-tree

    Check the age and condition of the filter if you can. They are extremely expensive to change.
  13. mimzy

    Edilbe mushroom cultures

    Hi folk, Due to a disaster in my laboratory (my fridge froze over) I'm currently looking for following strains; Pholiota nameko Pleurotus citrinopileatus (Golden oyster) Pleurotus pulmonarius (Blue oyster) Pleurotus djamor (Pink oyster) If anyone can help me out with some agar cultures or prints, I will be eternally grateful. I still have most of my strains to trade of course. Cheers, Mimz
  14. mimzy

    Edilbe mushroom cultures

    Will send nudes for cultures.
  15. mimzy

    Edilbe mushroom cultures

    Erg common, I know someone here has these. Your reward will be 2x pine seedling; 1. Inoculated with L. deliciosus (Saffron milk cap) 2. Inoculated with S. luteus. (Slippery Jack)
  16. mimzy

    Beating a cultivation charge?

    This thread is fucked on so many levels.
  17. mimzy

    Edilbe mushroom cultures

    Bump. Still looking for these.
  18. mimzy

    Edilbe mushroom cultures

    Thanks!
  19. mimzy

    Edilbe mushroom cultures

    Hi Worowa. Could give you whatever you like of the following; shiitake, king oyster, white oyster, enoki, lion's mane.
  20. mimzy

    Pasteurization of bulk substrate using Sous Vide

    1.5-2hrs is a normal length of time for pasteurisation of straw.
  21. mimzy

    wtb edible mushroom prints

    I don't have prints but I do have cultures. PM me if your interested.
  22. Hypothetically speaking , If I wanted to start a mushroom business, and I obtained my cultures from commercially available fruiting bodies, could I be exposing myself to legal repercussions later down the track? Are commercial strains normally patented in Australia? Thanks! Mimz
  23. Hi folk, I need a large chromatography column for an experiment I'm trying to finish before Christmas. I'm guessing a few of the lab rats here probably have a spare one. Must be at least 3x30cm, with tap. Cash money. Pm me if you can help me out. Cheers, Mimz
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